Molecular characterization and pathogenicity of very virulent infectious bursal disease virus isolated from naturally infected turkey poults in Egypt

Mosad, Samah M.; Eladl, Abdelfattah H.; El‐Tholoth, Mohamed; Ali, Hatem S.; Hamed, Mohamed F.

doi:10.1007/s11250-020-02420-5

Cited by 14 publications

(10 citation statements)

References 38 publications

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“…The indirect ELISA test result showed that all chicks revealed a small amount of the maternally-derived antibody that is within the negative range of the S/P ratio. Seven days post-inoculation of the antigen, the mean S/P ratio for Group 1 inoculated from passage 5 was 0.046 18). At day 21 post infections the S/P ratio or antibody titer seems equal with that of day 14 post-inoculation (Figure 4).…”

Section: Immunogenicity Of the Developed Vaccinementioning

confidence: 88%

“…The identity of the IBD virus vaccine strain adapted in Vero cell was checked by amplification of the viral hypervariable core protein (VP2) gene of the IBD virus using gene-specific primers by reverse transcription polymerase chain reaction (RT-PCR). 17 , 18 Sequencing of the amplified VP2 gene was conducted on vaccine working seed and from odd number passages 1, 3, 5, 7, 9 and 10 to see if there is any nucleotide variation. The Wizard ® SV Gel and PCR Clean-Up System (Promega, Germany) was used to purify the positive PCR products of the amplified VP2 gene.…”

Section: Methodsmentioning

confidence: 99%

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Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain

Kebede

Bitew²,

Bari

et al. 2021

VMRR

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Introduction Infectious bursal disease virus (IBDV) is an avian viral pathogen that causes infectious bursal disease (IBD) of chickens. The disease has been endemic in Ethiopia since 2002, and vaccination has been practiced as the major means of disease prevention and control. An IBD vaccine is produced in Ethiopia using primary chicken embryo fibroblast (CEF) cell, which is time-consuming, laborious, and uneconomical. The present study was carried out to develop cell-based IBDV LC-75 vaccine using Vero cells and to evaluate the safety, immunogenicity and protection level. Methods Identity of the vaccine seed was confirmed with gene-specific primers using reverse transcription polymerase chain reaction (RT-PCR). Confluent monolayer of Vero cells was infected with vaccine virus and serial passage continued till passage 10. A characteristic virus-induced cytopathic effect (CPE) was observed starting from passage 2 on the third day post-infection. The infectious titer of adapted virus showed a linear increment along the passage level. The virus-induced specific antibody was determined using indirect ELISA after vaccination of chicks through ocular route. Results The antibody titer measured from Vero cells vaccinated chicks revealed similar level with the currently available CEF cell-based vaccine, hence no significant difference. Chicks vaccinated with Vero cell adapted virus showed complete protection against very virulent IBDV, while unvaccinated group had 60% morbidity and 25% mortality. Conclusion It is concluded that the IBDV vaccine strain well adapted on Vero cells and found to be immunogenic induces antibody development and successfully protects chicks against challenge with the circulating field IBDV isolate. Hence, it is recommended to produce IBD vaccine using Vero cell culture at the industrial scale to conquer the limitations caused by using CEF cells and thus to vaccinate chicks population to protect against the circulating IBDV infection.

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Section: Immunogenicity Of the Developed Vaccinementioning

confidence: 88%

Section: Methodsmentioning

confidence: 99%

Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain

Kebede

Bitew²,

Bari

et al. 2021

VMRR

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“…3,6 This disease is caused by the IBD virus (IBDV) that belongs to the genus Avibirnavirus of the family Birnaviridae with a bi-segmented double-standard (ds) RNA genome. [7][8][9][10] IBDV is highly stable and resistant to many physical and chemical agents and prone to extreme antigenic variability with increased virulence. 11 The clinical signs of infected chickens relate to the age of the chickens and the virulence and the strain of the virus.…”

Section: Introductionmentioning

confidence: 99%

“…12 The disease can also infect other poults including turkeys. 9 Chickens are more susceptible at 3 to 6 weeks of age and get infected via the oro-faecal route in a direct mode of transmission since the virus is shed in high amounts in faeces from 48 hours up to 2 weeks postinfection. 13 There are two distinct serotypes of IBDV.…”

Section: Introductionmentioning

confidence: 99%

Comparative Immunogenicity Evaluation of Two Infectious Bursal Disease Vaccines Commonly Used in Broiler Chickens in Ethiopia

Legese

Wakjira

Teshome

et al. 2022

VMRR

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Purpose Infectious bursal disease (IBD) is one of the most endemic diseases of commercial poultry in Ethiopia. Vaccination is used as the major means of IBD prevention and control. A study was conducted to compare the immunogenicity of two commercially available IBD vaccines in broiler chicken with maternally derived antibody (MDA). Methods A total of 270 day-one-old chicks were randomly assigned to three groups, group 1 vaccinated with product A vaccine at the age of 7 and 19 days and group 2 with product B vaccine on day 15 and 22 while group 3 were kept as control. Six chickens were also randomly selected and bled on day 1 for differential leukocyte count (DLC) and determination of MDA. Representative chickens from each group were bled at 24th and 42nd days of age for antibody titration using the indirect ELISA test. DLC scores were determined in the 1st and 24th days. Results The result revealed highly significant differences ( P = 0.001) between group 1 and group 2 in DLC at 24th days of age. Antibody titers against IBD were differed significantly ( P = 0.02) at 24th and 42nd days of age in broilers vaccinated with product A and product B vaccines. Conclusion Both vaccines have induced an adequate immunological response at the end of the experiment; however, product A has shown significantly higher antibody titers against the IBDV and DLC than product B.

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“…Therefore, immunosuppression and weak immunity make chickens prone to various diseases and vaccination failure [ 5 ]. Immunomodulation is a major concern in poultry farms, and innovative products at reasonable rates that can help improve or avoid such diseases are critical for the poultry industry as well as humans [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of secondary metabolites of herbal plant extracts as an antiviral effect on infectious bursal disease virus isolates in embryonated chicken eggs

2021

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Background and Aim: Infectious bursal disease attacks the poultry industry, mainly young chickens, causing immunosuppression, and death with high economic losses. This study aimed to evaluate the effects of the monoextract, diextracts, and triextracts of Quercus infectoria (QI), Citrus aurantifolia (CiA), and Coffea arabica (CoA) on infectious bursal disease virus (IBDV) in embryonated chicken eggs (ECEs). Materials and Methods: The experimental design consisted of three sets of ECEs at 11 days of age, and each set included seven groups (G1-G7). The extracts of QI, CiA, and CoA were inoculated to ECEs by the chorioallantoic membrane method before, in concomitant (mixed) with, and after IBDV infection to the first, second, and third sets, respectively. The monoextract, diextracts, and triextracts of QI, CiA, and CoA were given at 1%, 2%, 5%, and 10% concentrations to G1-G3, G4-G6, and G7, respectively. Real-time polymerase chain reaction identified and confirmed the virus in accordance with the pathological changes. Results: The monoextract (5-10% concentrations) inhibited IBDV and had no effect on viral infection preinoculation, whereas the monoextract (10% concentration) inhibited IBDV during mixed inoculation and post-inoculation. Diextracts (2-10% concentrations) inhibited IBDV and had no effect on viral infection preinoculation, whereas diextracts (5-10% concentrations) inhibited IBDV during mixed inoculation and post-inoculation. Triextracts (1%, 2%, 5%, and 10% concentrations) inhibited IBDV by ameliorating the pathological changes of the virus and preventing the death of ECEs. Conclusion: The inoculation of herbal extracts, particularly triextracts, alleviates the pathological changes in ECEs infected with IBDV. This study recommends the oral route in evaluating plant extracts against IBDV in poultry.

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Molecular characterization and pathogenicity of very virulent infectious bursal disease virus isolated from naturally infected turkey poults in Egypt

Cited by 14 publications

References 38 publications

Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain

Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain

Comparative Immunogenicity Evaluation of Two Infectious Bursal Disease Vaccines Commonly Used in Broiler Chickens in Ethiopia

Evaluation of secondary metabolites of herbal plant extracts as an antiviral effect on infectious bursal disease virus isolates in embryonated chicken eggs

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