Molecular characterization and genotypic antimicrobial resistance analysis of<i>Campylobacter jejuni</i>and<i>Campylobacter coli</i>isolated from broiler flocks in northern Italy

Giacomelli, M.; Andrighetto, Christian; Rossi, Franca; Lombardi, Angiolella; Rizzotti, Lucia; Martini, Marco; Piccirillo, Alessandra

doi:10.1080/03079457.2012.734915

Cited by 22 publications

(22 citation statements)

References 38 publications

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“…The existence of high genotypic similarity amongst some strains isolated in Brazil is in contrast with previous studies conducted in several countries that showed high genomic diversity amongst C. coli strains (Harrington et al, 1997;Magnú sson et al, 2011;Giacomelli et al, 2012;Zhang et al, 2014). …”

Section: Discussioncontrasting

confidence: 52%

Genotyping of Campylobacter coli strains isolated in Brazil suggests possible contamination amongst environmental, human, animal and food sources

Gomes

Souza

Passaglia

et al. 2016

Journal of Medical Microbiology

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Campylobacter coli and Campylobacter jejuni are two of the most common causative agents of food-borne gastroenteritis in numerous countries worldwide. In Brazil, campylobacteriosis is underdiagnosed and under-reported, and few studies have molecularly characterized Campylobacter spp. in this country. The current study genotyped 63 C. coli strains isolated from humans (n512), animals (n521), food (n510) and the environment (n520) between 1995 and 2011 in Brazil. The strains were genotyped using pulsed-field gel electrophoresis (PFGE), sequencing the short variable region (SVR) of the flaA gene ( flaA-SVR) and high-resolution melting analysis (HRMA) of the clustered regularly interspaced short palindromic repeat (CRISPR) locus to better understand C. coli genotypic diversity and compare the suitability of these three methods for genotyping this species. Additionally, the discrimination index (DI) of each of these methods was assessed. Some C. coli strains isolated from clinical and non-clinical origins presented $80 % genotypic similarity by PFGE and flaA-SVR sequencing. HRMA of the CRISPR locus revealed only four different melting profiles. In total, 22 different flaA-SVR alleles were detected. Of these, seven alleles, comprising gt1647-gt1653, were classified as novel. The most frequent genotypes were gt30 and gt1647.This distribution reveals the diversity of selected Brazilian isolates in comparison with the alleles described in the PubMLST database. The DIs for PFGE, flaA-SVR sequencing and CRISPR-HRMA were 0.986, 0.916 and 0.550, respectively. PFGE and flaA-SVR sequencing were suitable for subtyping C. coli strains, in contrast to CRISPR-HRMA. The high genomic similarity amongst some C. coli strains confirms the hypothesis that environmental and food sources potentially lead to human and animal contamination in Brazil.

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Section: Discussioncontrasting

confidence: 52%

Genotyping of Campylobacter coli strains isolated in Brazil suggests possible contamination amongst environmental, human, animal and food sources

Gomes

Souza

Passaglia

et al. 2016

Journal of Medical Microbiology

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“…Each strain was isolated from different birds commercially reared in four broiler and three turkey farms throughout Northern Italy between 2009 and 2010. 20,21 Antimicrobial susceptibility determination Susceptibility testing was carried out by the disk diffusion method using Mueller-Hinton agar (OXOID, Basingstoke, United Kingdom) supplemented with 5% defibrinated sheep blood (OXOID). Isolates were tested for their susceptibility to 20 antimicrobial drugs belonging to nine classes: aminoglycosides ( Inocula were prepared from overnight growth on tryptic soy agar (OXOID) supplemented with 5% defibrinated sheep blood (OXOID) by suspension in sterile saline (0.9% w/v of NaCl), to obtain a turbidity equivalent to that of a 0.5 McFarland standard.…”

Section: Bacterial Strainsmentioning

confidence: 99%

Antimicrobial Resistance ofCampylobacter jejuniandCampylobacter colifrom Poultry in Italy

Giacomelli

Salata

Martini

et al. 2014

Microbial Drug Resistance

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This study was aimed at assessing the antimicrobial resistance (AMR) of Campylobacter isolates from broilers and turkeys reared in industrial farms in Northern Italy, given the public health concern represented by resistant campylobacters in food-producing animals and the paucity of data about this topic in our country. Thirty-six Campylobacter jejuni and 24 Campylobacter coli isolated from broilers and 68 C. jejuni and 32 C. coli from turkeys were tested by disk diffusion for their susceptibility to apramycin, gentamicin, streptomycin, cephalothin, cefotaxime, ceftiofur, cefuroxime, ampicillin, amoxicillin+clavulanic acid, nalidixic acid, flumequine, enrofloxacin, ciprofloxacin, erythromycin, tilmicosin, tylosin, tiamulin, clindamycin, tetracycline, sulfamethoxazole+trimethoprim, chloramphenicol. Depending on the drug, breakpoints provided by Comité de l'antibiogramme de la Société Française de Microbiologie, Clinical and Laboratory Standards Institute, and the manufacturer were followed. All broiler strains and 92% turkey strains were multidrug resistant. Very high resistance rates were detected for quinolones, tetracycline, and sulfamethoxazole+trimethoprim, ranging from 65% to 100% in broilers and from 74% to 96% in turkeys. Prevalence of resistance was observed also against ampicillin (97% in broilers, 88% in turkeys) and at least three cephalosporins (93-100% in broilers, 100% in turkeys). Conversely, no isolates showed resistance to chloramphenicol and tiamulin. Susceptibility prevailed for amoxicillin+clavulanic acid and aminoglycosides in both poultry species, and for macrolides and clindamycin among turkey strains and among C. jejuni from broilers, whereas most C. coli strains from broilers (87.5%) were resistant. Other differences between C. jejuni and C. coli were observed markedly in broiler isolates, with the overall predominance of resistance in C. coli compared to C. jejuni. This study provides updates and novel data on the AMR of broiler and turkey campylobacters in Italy, revealing the occurrence of high resistance to several antimicrobials, especially key drugs for the treatment of human campylobacteriosis, representing a potential risk for public health.

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“…One of these methods, flaA gene based PCR-RFLP is one of the widely used method for discrimination of C. jejuni and C. coli isolates [16] . Sequence analysis of short variable regions (SVRs) of flaA and flaB genes is another widely used method for genotyping of Campylobacter isolates, particularly in short-term and localized epidemiological studies [17][18][19] .…”

Section: Introductionmentioning

confidence: 99%

Broilerlerden İzole Edilen Termofilik Campylabacter’lerin Genotipik, Antimikrobiyal Direnç ve Virulens Profilleri

Aslantaş¹

2017

Kafkas Univ Vet Fak Derg

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The objective of this study was to investigate the prevalence, antimicrobial resistance and genetic determinants of resistance of Campylobacter jejuni and Campylobacter coli isolated from commercial broiler farms in Adana and Hatay provinces, Turkey. The assessment of the genetic diversity among the isolates was determined by flaA based RFLP-Polymerase Chain Reaction (PCR) with restriction enzyme DdeI and sequence analysis of short variable regions (SVRs) of flaA and flaB-SVR genes. Antimicrobial susceptibility of the isolates was performed by disk diffusion method and tetracycline (tetO), ampicillin (blaOXA-61), aminoglycoside (aph-3-1) and multidrug efflux pump (cmeB) resistance genes were investigated by PCR as well. The genes conferring resistance to ciprofloxacin was screened by PCR and following DNA sequencing. The presence of ten virulence (flaA, virB11, racR, cadF, ciaB, dnaJ and pldA) and toxin genes (cdtA, dtB, cdtC) among the isolates was also investigated using PCR. Out of 220 cloacal swabs, 218 (99.1%) Campylobacter spp. including C. jejuni (n=194; 89%) and C. coli (n=24; 11%) were isolated. While all the isolates were susceptible to chloramphenicol, gentamicin and erythromycin, resistance rates for C. jejuni and C. coli isolates to nalidixic acid, ciprofloxacin, ampicillin, tetracycline and amoxicillin-clavulanic acid were determined as 86.6-100%, 86.6-100%, 45.9-45.8%, 43.3-50% and 2.6-0.0%, respectively. The tetO, blaOXA-61, cmeB and aph-3-1 genes detected in C. jejuni and C. coli isolates were 45.3-54.2%, 36.1-75%, 1.5-83.3% and 0.5-0%, respectively.

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Molecular characterization and genotypic antimicrobial resistance analysis ofCampylobacter jejuniandCampylobacter coliisolated from broiler flocks in northern Italy

Cited by 22 publications

References 38 publications

Genotyping of Campylobacter coli strains isolated in Brazil suggests possible contamination amongst environmental, human, animal and food sources

Genotyping of Campylobacter coli strains isolated in Brazil suggests possible contamination amongst environmental, human, animal and food sources

Antimicrobial Resistance ofCampylobacter jejuniandCampylobacter colifrom Poultry in Italy

Broilerlerden İzole Edilen Termofilik Campylabacter’lerin Genotipik, Antimikrobiyal Direnç ve Virulens Profilleri

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