Molecular characterization and gene expression analysis of insulin-like growth factors I and II in the redbanded seabream, Pagrus auriga: transcriptional regulation by growth hormone

Ponce, Marian; Infante, Carlos; Funes, Victoria; Manchado, Manuel

doi:10.1016/j.cbpb.2008.04.013

Cited by 34 publications

(18 citation statements)

References 73 publications

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“…For IGF-I this is consistent with previous reports in fish including our own transgenic tilapia (Eppler et al 2007a) that GH treatment stimulates liver IGF-I gene expression. For IGF-II expression, no GH effect was found in gilthead seabream (Duguay et al 1996) but, in accordance with our results, GH increased IGF-II mRNA levels in rainbow trout, common carp and Japanese eel (Shamblott et al 1995;Vong et al 2003;Moriyama et al 2008), and in rainbow trout and redbanded seabream, similar to our findings in tilapia, more pronouncedly than IGF-I mRNA (Gahr et al 2008;Ponce et al 2008). IGF-II mRNA in muscle was similarly increased in the GH-overexpressing fish as found previously for IGF-I mRNA (Eppler et al 2007a) which suggests that expression of both IGFs in muscle is under control by GH.…”

Section: Discussionsupporting

confidence: 94%

Distinct organ-specific up- and down-regulation of IGF-I and IGF-II mRNA in various organs of a GH-overexpressing transgenic Nile tilapia

et al. 2009

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Several lines of GH-overexpressing fish have been produced and characterized concerning organ integrity, growth, fertility and health but few and contradictory data are available on IGF-I that mediates most effects of GH. Furthermore, nothing is known on IGF-II. Thus, the expression of both IGFs in liver and various extrahepatic sites of adult transgenic (GH-overexpressing) tilapia and age-matched wild-type fish was determined by real-time PCR. Both IGF-I and IGF-II mRNA were found in all organs investigated and were increased in gills, kidney, intestine, heart, testes, skeletal muscle and brain of the transgenics (IGF-I: 1.4-4-fold; IGF-II: 1.7-4.2-fold). Except for liver, brain and testis the increase in IGF-I mRNA was higher than that in IGF-II mRNA. In pituitary, no significant change in IGF-I or IGF-II mRNA was detected. In spleen, however, IGF-I and IGF-II mRNA were both decreased in the transgenics, IGF-I mRNA even by the 19-fold. In agreement, in situ hybridisation revealed a largely reduced number of IGF-I mRNA-containing leukocytes and macrophages when compared to wild-type. These observations may contribute to better understanding the reported impaired health of GH-transgenic fish. Growth enhancement of the transgenics may be due to the increased expression of both IGF-I and IGF-II in extrahepatic sites. It is also reasonable that the markedly enhanced expression of liver IGF-II mRNA that may mimick an early developmental stage is a further reason for increased growth.

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Section: Discussionsupporting

confidence: 94%

Distinct organ-specific up- and down-regulation of IGF-I and IGF-II mRNA in various organs of a GH-overexpressing transgenic Nile tilapia

et al. 2009

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“…However, in many of the studies aimed at assessing the effects of GH, hepatic igf1 mRNA measurements were made within hours of GH administration. A recent study by Moriyama et al (2006) on effects of recombinant GH in glass eel of Anguilla japonica and by Ponce et al (2008) on redbanded seabream, Pagrus auriga, has revealed that increased hepatic mRNA levels of igf1 were maintained for only up to 1 day post GH treatment. A similar trend was obtained in perch, Perca fluviatilis, in which GH failed to elicit any changes at all with regard to hepatic igf1 mRNA (Jentoft et al 2004); when measurements were made at later time points, GH effects were typically not significant, e.g.…”

Section: Discussionmentioning

confidence: 99%

Effects of reproductive stage, GH, and 11-ketotestosterone on expression of growth differentiation factor-9 in the ovary of the eel, Anguilla australis

Lokman¹,

Kazeto²,

Ozaki³

et al. 2010

Reproduction

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In order to study the regulation of the growth differentiation factor-9 (gdf9) gene in a primitive teleost with semelparous life history, we cloned a cDNA encoding shortfinned eel Gdf9, expressed a partial peptide in Escherichia coli, and raised an antiserum to evaluate changes in Gdf9 expression during its pituitary homogenate-induced reproductive cycle. The effects of in vivo and in vitro exposure to the androgen 11-ketotestosterone (11-KT), known to affect previtellogenic (PV) oocyte growth, were also determined. Furthermore, we investigated whether Gdf9 expression was metabolically gated by treating PV fish with recombinant GH in vivo. Immunoreactive proteins of ca. 52 and 55 kDa were identified by western blot analysis. Gdf9 message and protein were most abundant in PV oocytes, and peaked slightly earlier for mRNA than for protein. Captivity resulted in reduced gdf9 mRNA levels, which were restored following pituitary homogenate treatment. As oocytes progressed through induced oogenesis, Gdf9 expression decreased. Neither 11-KT nor GH treatment affected gdf9 mRNA levels in PV fish, although GH could partially restore handling-or captivity-induced decreases in gdf9 mRNA levels. Semelparous eels thus show an expression pattern of Gdf9 during oogenesis that is similar to that seen in other vertebrates, that appears responsive to handling or captivity stress, and whose control remains to be elucidated.

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“…Recent studies show that teleost fishes including salmonids, sea bream, carp, catfish, eels, rabbitfish, sea bass, and hybrid striped bass, as well as the dogfish, an elasmobranch, differ from mammals in that liver igf2 gene expression and plasma Igf2 levels are stimulated by GH and respond to metabolic status (Shamblott et al 1995, Tse et al 2002, Vong et al 2003a, Peterson et al 2004, Carnevali et al 2005, Gabillard et al 2006, Wilkinson et al 2006, Ayson et al 2007, Terova et al 2007, Gahr et al 2008, Moriyama et al 2008a, b, Picha et al 2008b, Ponce et al 2008, Devlin et al 2009, Peterson & Waldbieser 2009, Eppler et al 2010, Pierce et al 2010. In primary cultured coho salmon hepatocytes, insulin directly increased igf2 mRNA, and strongly increased the response of igf2 mRNA to GH, but suppressed the response of igf1 mRNA to GH (Pierce et al 2005(Pierce et al , 2010.…”

Section: Introductionmentioning

confidence: 99%

Differential regulation of Igf1 and Igf2 mRNA levels in tilapia hepatocytes: effects of insulin and cortisol on GH sensitivity

Pierce

Breves²,

Moriyama³

et al. 2011

Journal of Endocrinology

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Igf1 and Igf2 stimulate growth and development of vertebrates. In mammals, liver-derived endocrine Igf1 mediates the growth promoting effects of GH during postnatal life, whereas Igf2 stimulates placental and fetal growth and is not regulated by GH. Insulin enhances Igf1 production by the mammalian liver directly, and by increasing hepatocyte sensitivity to GH. We examined the regulation of igf1 and igf2 mRNA levels by GH, insulin, and cortisol, and the effects of insulin and cortisol on GH sensitivity in primary cultured hepatocytes of tilapia, a cichlid teleost. GH increased mRNA levels of both igf1 and igf2 in a concentration-related and biphasic manner over the physiological range, with a greater effect on igf2 mRNA level. Insulin increased basal igf2 mRNA level, and strongly increased GH-stimulated igf2 mRNA level, but slightly reduced basal igf1 mRNA level and did not affect GH-stimulated igf1 mRNA level. Cortisol inhibited GH stimulation of igf1, but increased GH stimulation of igf2 mRNA level. The synergistic effect of insulin and GH on igf2 mRNA level was confirmed in vivo. These results indicate that insulin and cortisol differentially modulate the response of igf1 and igf2 mRNA to GH in tilapia hepatocytes, and suggest that the regulation of liver Igf2 production differs between fish and mammals. Regulation of liver Igf2 production in fish appears to be similar to regulation of liver Igf1 production in mammals.

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Molecular characterization and gene expression analysis of insulin-like growth factors I and II in the redbanded seabream, Pagrus auriga: transcriptional regulation by growth hormone

Cited by 34 publications

References 73 publications

Distinct organ-specific up- and down-regulation of IGF-I and IGF-II mRNA in various organs of a GH-overexpressing transgenic Nile tilapia

Distinct organ-specific up- and down-regulation of IGF-I and IGF-II mRNA in various organs of a GH-overexpressing transgenic Nile tilapia

Effects of reproductive stage, GH, and 11-ketotestosterone on expression of growth differentiation factor-9 in the ovary of the eel, Anguilla australis

Differential regulation of Igf1 and Igf2 mRNA levels in tilapia hepatocytes: effects of insulin and cortisol on GH sensitivity

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