Molecular characterization and functional analysis of the ultraspiracle (USP) in the oriental fruit moth Grapholita molesta (Lepidoptera: Olethreutidae)

Cao, Jinjun; Liu, Yichen; Yang, Yuhui; Zhang, Huaijiang; Li, Zhen; Yang, Qingliang; Zhang, Songdou; Zhang, Qingwen; Liu, Xiaoxia

doi:10.1016/j.cbpb.2015.08.008

Cited by 23 publications

(24 citation statements)

References 51 publications

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“…In this paper, we found that the expression levels of LdEcRA , LdEcRB , LdHR3 , LdHR4 , LdE74 and LdE75 were significantly decreased in LdUSP RNAi larvae in L. decemlineata . Consistent with our results, E74 , E75 , HR3 , HR4 , βFTZ‐F1 , EcR and BrC are significantly downregulated after GmUSP silencing in G. molesta (Cao et al , ). When the function of USP is inhibited in D. melanogaster , the expression levels of EcRB1 , E75B and HR3 are severely reduced (Schubiger and Truman, ).…”

Section: Discussionsupporting

confidence: 92%

“…These results clearly confirm a crucial role of EcR/USP heterodimer in successful completion of the moulting process in L. decemlineata, similar to other insect species (Oro et al, 1992;Martin et al, 2006;Tan and Palli, 2008;Cao et al, 2015;Lenaerts et al, 2016).…”

Section: Knockdown Of Ldusp Impairs Pupal Moultingsupporting

confidence: 85%

“…In agreement with our results, loss of maternal or zygotic USP functions in D. melanogaster lead to embryonic lethality (Henrich et al , ; Ghbeish et al , ), or death at the first larval moult (Oro et al , ). Moreover, knockdown of USP causes larval lethality during the quiescent stage in T. castaneum (Tan and Palli, ), impairs pupation and adult emergence in G. molesta (Cao et al , ) and results in moulting failure in B. germanica (Martin et al , ) and S. gregaria (Lenaerts et al , ).…”

Section: Discussionmentioning

confidence: 99%

“…In the presence of 20E, USP heterodimerizes EcR to activate a subset of primary 20E response genes (E74, E75, HR3, HR4 and FTZ-F1, among others) that encode transcription factors to control signal transduction (Buszczak and Segraves, 2000). Mutations in or RNA interference (RNAi) of USP in the common fruit fly Drosophila melanogaster (Oro et al, 1992), the red flour beetle Tribolium castaneum (Tan and Palli, 2008), the oriental fruit moth Grapholita molesta (Cao et al, 2015), the brown planthopper Nilaparvata lugens (Xu et al, 2017), the desert locust Schistocerca gregaria (Lenaerts et al, 2016) and the German cockroach Blattella germanica (Martin et al, 2006) reveal that USP is necessary for the mediation of 20E signalling to initiate juvenile development in both holometabolans and hemimetabolans.…”

Section: Introductionmentioning

confidence: 99%

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Functional characterization of ultraspiracle in Leptinotarsa decemlineata using RNA interference assay

Deng

et al. 2019

Insect Molecular Biology

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A heterodimer of ultraspiracle (USP) and ecdysone receptor (EcR) mediates 20‐hydroxyecdysone (20E) signalling cascade to regulate insect moulting and metamorphosis. However, at least two questions remain to be addressed in terms of the molecular importance of USP in insect species. First, is USP involved in both regulation of ecdysteroidogenesis and mediation of 20E signalling in non‐drosophilid insects, as in Drosophila melanogaster? Second, does USP play any role in larval metamorphosis except as the partner of heterodimeric receptor to activate the downstream 20E signalling genes? In this paper, we found that RNA interference (RNAi) of LdUSP in the final (fourth) instar larvae reduced the messenger RNA levels of four ecdysteroidogenesis genes (Ldspo, Ldphm, Lddib and Ldsad) and 20E titre, and repressed the expression of five 20E signal genes (EcRA, HR3, HR4, E74 and E75) in Leptinotarsa decemlineata. The LdUSP RNAi larvae remained as prepupae, with developing antennae, legs and discs of forewings and hindwings. Dietary supplement with 20E restored the expression of the five 20E signal genes, but only partially alleviated the decreased pupation rate in LdUSP RNAi beetles. Knockdown of LdUSP at the penultimate (third) instar larvae did not affect third–fourth instar moulting. However, silencing LdUSP caused similar but less severe impairments on pupation. Accordingly, we propose that USP is undoubtedly necessary for ecdysteroidogenesis, for mediation of 20E signalling and for initiation of metamorphosis in L. decemlineata.

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Section: Discussionsupporting

confidence: 92%

Section: Knockdown Of Ldusp Impairs Pupal Moultingsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Functional characterization of ultraspiracle in Leptinotarsa decemlineata using RNA interference assay

Deng

et al. 2019

Insect Molecular Biology

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“…In this context, the development of new methods for insect control is of key importance and there has been intense interest in the utility of gene silencing methods induced by RNA interference (RNAi). RNAi can induce mortality (Yang & Han, ; Cao et al., ; Abd El Halim et al., ; Christiaens et al., ; Hu et al., ; Malik et al., ), create beneficial phenotypes for insect control (Salvemini et al., ; Shukla & Palli, ; Peng et al., ; Yu et al., ), and prevent pesticide resistance in insect pests (Figueira‐Mansur et al., ; Guo et al., ; Wei et al., ; Bona et al., ; Sandoval‐Mojica & Scharf, ). Therefore, the potential for RNAi as a basis for future pest management strategies holds great promise (Huvenne & Smagghe, ; Gu & Knipple, ; Scott et al., ; Baum & Roberts, ; Kim et al., ).…”

Section: Rnai and The Sterile Insect Technique (Sit)mentioning

confidence: 99%

Implementing the sterile insect technique with RNA interference – a review

Darrington

Dalmay

Morrison

et al. 2017

Entomologia Exp Applicata

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We review RNA interference (RNAi) of insect pests and its potential for implementing sterile insect technique (SIT)‐related control. The molecular mechanisms that support RNAi in pest species are reviewed in detail, drawing on literature from a range of species including Drosophila melanogaster Meigen and Homo sapiens L. The underlying genes that enable RNAi are generally conserved across taxa, although variance exists in both their form and function. RNAi represents a plausible, non‐GM system for targeting populations of insects for control purposes, if RNAi effector molecules can be delivered environmentally (eRNAi). We consider studies of eRNAi from across several insect orders and review to what extent taxonomy, genetics, and differing methods of double‐stranded (ds) RNA synthesis and delivery can influence the efficiency of gene knockdown. Several factors, including the secondary structure of the target mRNA and the specific nucleotide sequence of dsRNA effector molecules, can affect the potency of eRNAi. However, taxonomic relationships between insects cannot be used to reliably forecast the efficiency of an eRNAi response. The mechanisms by which insects acquire dsRNA from their environment require further research, but the evidence to date suggests that endocytosis and transport channels both play key roles. Delivery of RNA molecules packaged in intermediary carriers such as bacteria or nanoparticles may facilitate their entry into and through the gut, and enable the evasion of host defence systems, such as toxic pH, that would otherwise attenuate the potential for RNAi.

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Molecular and functional characterization of three odorant binding proteins from the oriental fruit moth Grapholita molesta (Busck) (Lepidoptera: Tortricide)

Xiu-lin

Су

et al. 2018

Arch Insect Biochem Physiol

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Odorant binding proteins (OBPs) act in recognizing odor molecules and their most well-studied functions are transporting odors across the sensillum lymph to olfactory receptor neurons within the insect antennal sensillum. The adults of Grapholita molesta highly depend on olfactory cues in locating host plants and selecting oviposition sites, in which OBPs play an important role in perceiving and recognizing host plant volatiles. Exploring the physiological function of OBPs could facilitate our understanding of their importance in insects' chemical communication. In this study, three OBP genes were cloned and named GmolOBP4, GmolOBP5, and GmolOBP10. Quantitative real-time PCR results indicated that GmolOBP4 and GmolOBP10 were predominantly expressed in adult antennae and GmolOBP5 was expressed in multiple tissues, including head, legs, and wings in addition to antennae. The binding affinities of the three recombinant GmolOBPs (rGmolOBPs) with four sex pheromone components and twenty-nine host plant volatiles were measured using 1-N-Phenyl-naphthylamine as a fluorescence probe. The three rGmolOBPs exhibited specific binding properties to potential ligands, GmolOBP4 and GmolOBP10 bound to minor sex pheromone components, such as (Z)-8-dodecenyl alcohol and dodecanol, respectively. rGmolOBP4 showed intermediate binding ability with hexanal, benzyl alcohol, and pear ester, rGmolOBP5 had a weak affinity for benzaldehyde, pear ester and, methyl jasmonate, and rGmolOBP10 showed strong binding capacity toward hexanol, decanol, and α-ocimene. We speculate that the GmolOBP4 and GmolOBP10 have dual functions in perception and recognition of host plant volatiles and sex pheromone components, while GmolOBP5 may serve other function(s).

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Molecular characterization and functional analysis of the ultraspiracle (USP) in the oriental fruit moth Grapholita molesta (Lepidoptera: Olethreutidae)

Cited by 23 publications

References 51 publications

Functional characterization of ultraspiracle in Leptinotarsa decemlineata using RNA interference assay

Functional characterization of ultraspiracle in Leptinotarsa decemlineata using RNA interference assay

Implementing the sterile insect technique with RNA interference – a review

Molecular and functional characterization of three odorant binding proteins from the oriental fruit moth Grapholita molesta (Busck) (Lepidoptera: Tortricide)

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