Molecular characterisation of cytochrome P450 enzymes in waterflea (Daphnia pulex) and their expression regulation by polystyrene nanoplastics

Wu, Donglei; Liu, Zhiquan; Cai, Mingqi; Jiao, Yang; Li, Yiming; Chen, Qiang; Zhao, Yunlong

doi:10.1016/j.aquatox.2019.105350

Cited by 45 publications

(18 citation statements)

References 56 publications

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“…Nine studies used two different techniques each, and three studies used more than two techniques each to characterize the size of the studied polystyrene nanoparticles before adding them into the corresponding test medium. In the test media, the characterization of the polystyrene nanoparticle sizes was done with two or more techniques in four studies; these studies used DLS and transmission electron microscopy (TEM) 59,60 or DLS, TEM and scanning electron microscope (SEM) and DLS and electrophoretic light scattering (ELS).…”

Section: Critical Reviewmentioning

confidence: 99%

“…In fact, this was performed in several of the reviewed studies (10 out of 25) before the polystyrene nanoparticles were added to the test medium. 24,40,59,60,[72][73][74][75] Other techniques, such as NTA or differential centrifugal sedimentation (DCS), can be used to characterize the size of larger polystyrene nanoparticles. While DLS measures a bulk of particles with a strong bias to the largest particles present in the sample, NTA is based on the tracking of single particles.…”

Section: Critical Reviewmentioning

confidence: 99%

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Review of ecotoxicological studies of widely used polystyrene nanoparticles

Kelpsiene

Ekvall

Lundqvist

et al. 2022

Environ. Sci.: Processes Impacts

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Section: Critical Reviewmentioning

confidence: 99%

Section: Critical Reviewmentioning

confidence: 99%

Review of ecotoxicological studies of widely used polystyrene nanoparticles

Kelpsiene

Ekvall

Lundqvist

et al. 2022

Environ. Sci.: Processes Impacts

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“…In daphnids, upregulation of certain cytochrome P450 genes were reported upon chronic exposure to acetaminophen (Kim et al, 2018) or to polystyrene nanoplastics (Wu et al, 2019). In addition, net inhibition toward cytochrome P450 activity (ECOD) by azole fungicides was demonstrated by Dalhoff et al, (2016).…”

Section: Discussionmentioning

confidence: 99%

Physiological and metabolic alterations induced by commercial neonicotinoid formulations in Daphnia magna

et al. 2022

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Neonicotinoid insecticides are widely used agents in agriculture to control a broad range of insect pests. Although use of neonicotinoid pesticides has resulted in the widespread contamination of surface waters, sublethal toxicity data of these products in relation to nontarget aquatic biota are still poor. Therefore, the objective of this study was to assess the effects of two neonicotinoid pesticides with widespread use on the basic physiological functions: the thoracic limb activity and heart rate of Daphnia magna, and to screen for their potential to affect the cytochrome P450 monooxygenase system (ECOD activity) of daphnids. The considered pesticides were the acetamiprid-and thiacloprid based products Mospilan 20 SG and Calypso 480 SC, respectively. The dose-dependent variation in the three biological endpoints considered were assessed following 24h exposures. The two neonicotinoid formulations elicited significant depression on the thoracic limb activity and heart rate of daphnids at doses close to the immobility thresholds of formulations (48h-EC50: 2 Mospilan 20 SG = 190 mg L -1 ; Calypso 480 SC = 120 mg L -1 ), an effect mainly attributable to the overall drop in the general health status of the organisms. The alterations in the physiological traits were significant at exposures to 190 mg L -1 for Mospilan 20 SG and 48 mg L -1 for Calypso 480 SC. The dose related variation in the ECOD activity of daphnids exposed to the selected neonicotinoid formulations followed a biphasic pattern, with starting effective doses for Mospilan 20 SG of 6.3 mg L -1 (= 1/20 of 48h-EC50 for Daphnia neonates), and for Calypso 480 SC of 0.034 mg L -1 (= 1/4000 of 48h-EC50). Maximal ECOD activity (2.2 fold increase vs. controls) was induced by Mospilan 20 SG in daphnids exposed to 114 mg L -1 product (= 48h-EC20), and by Calypso 480 SC (1.8 fold increase) at 5.2 mg L -1 dose (= 1/20 of 48h-EC50). Our results outlined significant alterations in the physiological traits and ECOD activity in exposed daphnids at concentrations below the immobility thresholds (48h-EC50) of the products used as benchmarks to rate their toxicity risks to aquatic biota. Therefore, we think our findings might deserve consideration in the environmental risk evaluation of these products.

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“…The D. magna detoxification mechanism is an important area for research, both for detecting targeted xenobiotics and for understanding its defense mechanisms to xenobiotics in a natural environment. Though several studies have revealed the up‐regulation of many CYP genes in response to xenobiotics in Daphnia (Le et al 2010; Wang et al 2016; Schmidt et al 2017; Wu et al 2019), there has been no information on the temporal assessment of the basal expression level of detoxification‐related genes. No CYP mutant has been established for understanding the molecular mechanism of xenobiotic detoxification in Daphnia .…”

Section: Discussionmentioning

confidence: 99%

Mutation of the Cytochrome P450 CYP360A8 Gene Increases Sensitivity to Paraquat in Daphnia magna

Religia

Nguyen

Nong

et al. 2021

Enviro Toxic and Chemistry

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The freshwater crustacean Daphnia magna has traditionally been a model for ecotoxicological studies owing to its sensitivity to many xenobiotics. Because it is used in many toxicity assessments, its detoxification mechanism for xenobiotics is important and requires further study. However, studies related to detoxification genes are limited to transcriptomic profiling, and there are no D. magna mutants for use in the understanding of xenobiotic metabolism in vivo. We report the generation of a D. magna CYP360A8 mutant—the gene is a cytochrome P450 (CYP) clan 3 gene. Based on RNA sequencing of adult D. magna, we found that CYP360A8 has the highest expression level among all CYP genes. At ovarian maturation, its expression level is up‐regulated 6‐fold compared to the juvenile stages and is maintained thereafter. Using the CRISPR/CRISPR‐associated 9 (Cas9) system, we disrupted CYP360A8 by coinjecting CYP360A8‐targeting guide RNA and Cas9 proteins into D. magna eggs and established one monoallelic CYP360A8 mutant line. This CYP360A8 mutant had a higher sensitivity to the herbicide paraquat compared to the wild type. We confirmed the up‐regulation of CYP360A8 by paraquat. The results demonstrate the role of CYP360A8 in paraquat detoxification. The present study establishes a CYP mutant of D. magna, and this strategy can be a basic platform to document a range of CYP gene–xenobiotic relationships in this species. Environ Toxicol Chem 2021;40:1279–1288. © 2020 SETAC

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Molecular characterisation of cytochrome P450 enzymes in waterflea (Daphnia pulex) and their expression regulation by polystyrene nanoplastics

Cited by 45 publications

References 56 publications

Review of ecotoxicological studies of widely used polystyrene nanoparticles

Review of ecotoxicological studies of widely used polystyrene nanoparticles

Physiological and metabolic alterations induced by commercial neonicotinoid formulations in Daphnia magna

Mutation of the Cytochrome P450 CYP360A8 Gene Increases Sensitivity to Paraquat in Daphnia magna

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