Molecular biodiversity of Red Sea demosponges

Erpenbeck, Dirk; Voigt, Oliver; Al-Aidaroos, Ali M.; Berumen, Michael L.; Büttner, Gabriele; Catania, Daniela; Guirguis, Adel Naguib; Paulay, Gustav; Schätzle, Simone; Wörheide, Gert

doi:10.1016/j.marpolbul.2015.12.004

Cited by 47 publications

(37 citation statements)

References 64 publications

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“…Examination of the type specimen revealed clavulate acanthostyles characteristic for the genus Ectyoplasia . The morphological identification was later confirmed with molecular techniques by Erpenbeck et al () as OTU030. This species has been observed from the Red Sea, Mauritius, and western Thailand by the first author.…”

Section: Methodsmentioning

confidence: 94%

Assessing the bacterial communities of sponges inhabiting the remote western Indian Ocean island of Mayotte

et al. 2018

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Marine sponges are known to host diverse and abundant communities of microbial symbionts. It has been generally assumed that the bacterial communities of low microbial abundance (LMA) sponges are less diverse than those of high microbial abundance (HMA) sponges. In this study, we used next‐generation sequencing technology to explore the bacterial communities of several biotopes including sponges, seawater, and sediment from the remote Western Indian Ocean island of Mayotte. The species investigated were the known LMA sponges: Jaspis splendens, Stylissa carteri, and Stylissa massa, and the known HMA sponges: Hyrtios erectus and Xestospongia testudinaria. In addition to this, we also investigated the following sponge species: Ectyoplasia coccinea, Paratetilla bacca, Liosina paradoxa, and Petrosia aff. spheroida of which the exact HMA/LMA status is unknown although we preliminarily classified them as HMA or LMA based on the status of closely related species. Certain HMA sponges shared similar bacterial communities dominated by Actinobacteria and Chloroflexi, whereas another species (E. coccinea) had a bacterial community closer to that of LMA sponges. Most LMA sponges housed a bacteriome dominated by Proteobacteria and Cyanobacteria, but the bacteriome of P. bacca also included dominant Chloroflexi and actinobacterial OTUs. Together with S. carteri, this sponge housed a more diverse bacterial community at the phylum, class, and order levels than HMA sponges. Although certain LMA sponges housed a bacterial community similar to the surrounding environment (seawater), they also included highly abundant, possibly species or genus specific, OTUs. Based on this study and small set of sponges studied, we conclude that a clear dichotomy between HMA and LMA sponges does not appear to exist.

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Section: Methodsmentioning

confidence: 94%

Assessing the bacterial communities of sponges inhabiting the remote western Indian Ocean island of Mayotte

et al. 2018

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“…A significant body of research has been undertaken for reef fishes and other common sessile organisms like corals or sponges in the Red Sea (e.g., Berumen et al, 2013;Loya et al, 2014;Erpenbeck et al, 2016;Roberts et al, 2016), identifying it as a region of high biodiversity and endemism. However, zooplankton biodiversity remains largely uncharacterized.…”

Section: Characterization Of Zooplankton Biodiversity Using Pyrosequementioning

confidence: 99%

Metabarcoding Reveals Seasonal and Temperature-Dependent Succession of Zooplankton Communities in the Red Sea

2017

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Very little is known about the composition and the annual cycle of zooplankton assemblages in the Red Sea, a confined water body characterized by a high biodiversity and endemism but at the same time one of the most understudied areas in the world in terms of marine biodiversity. This high diversity together with the lack of references for several of the groups poses a problem in obtaining basic information on zooplankton seasonal patterns. In the present work, we used high throughput sequencing to examine the temporal and spatial distribution of the zooplankton communities inhabiting the epipelagic zone in the central Red Sea. The analysis of zooplankton assemblages collected at two sites-coastal and offshore-twice a month at several depth strata by using MANTA, Bongo and WP2 nets provides baseline information of the seasonal patterns of the zooplankton community over 1 year. We show that the seasonal fluctuation of zooplankton communities living in the upper 100 m of the water column is driven mainly by the annual changes in seawater temperature. The 18S rRNA gene was used for metabarcoding of zooplankton assemblages revealing 630 metazoan OTUs (97% similarity) in five phyla, highlighting the richness of the Red Sea community. During colder months, communities were characterized by lower richness and higher biomass than communities found during the hot season. Throughout the year the zooplankton communities were dominated by the class Maxillopoda, mainly represented by copepods and class Hydrozoa. The rise in the water temperature favors the appearance of classes Malacostraca, Cephalopoda, Gastropoda, and Saggitoidea. The present study provides essential baseline information for future monitoring and improves our knowledge of the marine ecosystem in the Red Sea while reporting the main environmental variable structuring zooplankton assemblages in this region.

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“…Despite highlighting the polyphyletic nature of Haplosclerida, applying a multi-locus based approach using ribosomal and mitochondrial markers continues to prove as a useful tool in resolving the taxonomy between congeneric species. Recently this approach has been used across a wide taxonomic range of sponges (Erpenbeck et al 2016;Yang et al 2017). These methods are useful as a first pass assessment of classification for a wide range diversity of sponges, to be subsequently integrated with morphological systematics.…”

Section: Discussionmentioning

confidence: 99%

Peer Review #1 of "Molecular and morphological congruence of three new cryptic Neopetrosia spp. in the Caribbean (v0.1)"

2019

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Neopetrosia proxima (Porifera: Demospongiae: Haplosclerida) is described as a morphologically variable sponge common on shallow reefs of the Caribbean. However, the range of morphological and reproductive variation within putative N. proxima led us to hypothesize that such variability may be indicative of cryptic species rather than plasticity. Using DNA sequences and morphological characters we confirmed the presence of three previously undescribed species of Neopetrosia. Morphological differences of each new congener were best resolved by partial gene sequences of the mitochondrial cytochrome oxidase subunit 1 (COI) over nuclear ones (18S rRNA and 28S rRNA). Several new characters for Neopetrosia were revealed by each new species. For example, Neopetrosia dendrocrevacea sp. nov. and Neopetrosia cristata sp. nov. showed the presence of grooves on the surface of the sponge body that converge at the oscula, and a more disorganized skeleton than previously defined for the genus. Neopetrosia sigmafera sp. nov. adds the 1) presence of sigma microscleres, 2) significantly wider/longer oxeas (>200 µm), and 3) the presence of parenchymella larvae. Sampling of conspecifics throughout several locations in the Caribbean revealed larger spicules in habitats closer to the continental shelf than those in remote island locations. Our study highlights the importance of integrating molecular and morphological systematics for the discrimination of new Neopetrosia spp. despite belonging to one of several polyphyletic groups (families, genera) within the current definition of the order Haplosclerida.

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Molecular biodiversity of Red Sea demosponges

Cited by 47 publications

References 64 publications

Assessing the bacterial communities of sponges inhabiting the remote western Indian Ocean island of Mayotte

Assessing the bacterial communities of sponges inhabiting the remote western Indian Ocean island of Mayotte

Metabarcoding Reveals Seasonal and Temperature-Dependent Succession of Zooplankton Communities in the Red Sea

Peer Review #1 of "Molecular and morphological congruence of three new cryptic Neopetrosia spp. in the Caribbean (v0.1)"

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