Molecular Basis of Stationary Phase Survival and Applications

Jaishankar, Jananee; Srivastava, Preeti

doi:10.3389/fmicb.2017.02000

Cited by 202 publications

(187 citation statements)

References 89 publications

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“…The productions of proteins at stationary phase may be linked with the need of the bacterium to combat complex environments present in the stationary phase including altered pH and osmolality. It has been reported that the production of proteins at stationary phase increased by 121% as compared with exponential phase (Ou et al, 2004;Jaishankar and Srivastava, 2017). The release of amino acids in this study may relate to peptidoglycan synthesis and quorum-sensing system (De Kievit et al, 2001;Aliashkevich et al, 2018).…”

Section: Discussionmentioning

confidence: 52%

“…This clearly indicated that regardless of alterations in environmental factors applied in this study, the different phases of growth inferred the most influence on uptake characteristics. Stationary phase is considered as a complex environment, and physiologically is comparable to biofilm (Jaishankar and Srivastava, 2017). In stationary phase, extraordinary adjustments take place including morphological, physiological changes and DNA/protein ratio was found to be higher (Nystrom, 2004;Jaishankar and Srivastava, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the use of NaCl in processed and canned foods may increase biofilm production of S. aureus (Lee et al, 2014). Further, biofilm formation can occur in response to other non-ideal growth conditions such as exposure to alkaline environments (Jones et al, 2015;Jaishankar and Srivastava, 2017). Both SCV phenotypes and biofilm greatly contribute to the profound resistance and stable adhesion, which eventually assist the bacteria to survive under changed environmental conditions (Yao et al, 2005;Li et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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The Uptake and Release of Amino Acids by Staphylococcus aureus at Mid-Exponential and Stationary Phases and Their Corresponding Responses to Changes in Temperature, pH and Osmolality

et al. 2020

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Staphylococcus aureus is an important pathogen that is associated with nosocomial infections, as well as food poisoning. This bacterium is resistant to antimicrobial agents and can survive in a wide range of environmental conditions. The aim of this study was to measure the uptake and release of amino acids by S. aureus at midexponential and stationary phases of growth following exposure to a combination of conditions including variations in temperature, pH and NaCl. Bacterial cells were grown up to mid-exponential and stationary phases in tryptic soy broth (TSB), where the supernatants were collected for analyses of amino acids to determine the uptake and release characteristics. The uptake/release of amino acids was estimated by subtracting the initial levels of the free amino acids in the media from those measured at mid-exponential and stationary phases of growth. When cells were grown at ideal conditions, the analyses revealed that significant uptake of amino acids had occurred by stationary phase compared with the mid-exponential phase. A substantial release of valine and tyrosine into the external media was observed by cells at stationary phase. At both phases, the uptake and release patterns were significantly different between cells grown under ideal control conditions, when compared with those grown under various combinations of sub-optimal environmental conditions. The analyses of the supernatants harvested from controls and treatment groups at exponential phase indicated that the total uptake of amino acids was reduced approximately five times by cells grown with addition of 2.5% NaCl or with pH6 at 35 • C, and 2-fold by cells grown at pH8 at 35 • C. However, the final quantities of amino acids taken up by cells grown to stationary phase did not significantly alter between control and treated samples. Valine was found to be the most abundant amino acid that was significantly released into the media at stationary phase by both control and treated samples. It was evident that diverse environmental conditions resulted in differential patterns of amino acid uptake and release during adaptation to designated conditions.

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Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Uptake and Release of Amino Acids by Staphylococcus aureus at Mid-Exponential and Stationary Phases and Their Corresponding Responses to Changes in Temperature, pH and Osmolality

et al. 2020

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“…During the stationary phase, prokaryotes undergo several morphological and physiological changes that increase their stress resistance and allow them to survive under severe nutrient restrictions. These include the development of smaller and spherical cells, a rigid cell envelope, nucleoid condensation, a reduction in protein synthesis, and an increase in peptidases/proteases . Very few studies have explored the transition to stationary phase at a global scale in Archaea and to the best of our knowledge, only two have done it at the proteome level: in the thermophile Thermococcus kodakarensis and H. marismortui …”

mentioning

confidence: 99%

Proteomic Study of the Exponential–Stationary Growth Phase Transition in the Haloarchaea Natrialba magadii and Haloferax volcanii

et al. 2018

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The dynamic changes that take place along the phases of microbial growth (lag, exponential, stationary, and death) have been widely studied in bacteria at the molecular and cellular levels, but little is known for archaea. In this study, a high-throughput approach was used to analyze and compare the proteomes of two haloarchaea during exponential and stationary growth: the neutrophilic Haloferax volcanii and the alkaliphilic Natrialba magadii. Almost 2000 proteins were identified in each species (≈50% of the predicted proteome). Among them, 532 and 432 were found to be differential between growth phases in H. volcanii and N. magadii, respectively. Changes upon entrance into stationary phase included an overall increase in proteins involved in the transport of small molecules and ions, stress response, and fatty acid catabolism. Proteins related to genetic processes and cell division showed a notorious decrease in amount. The data reported in this study not only contributes to our understanding of the exponential-stationary growth phase transition in extremophilic archaea but also provides the first comprehensive analysis of the proteome composition of N. magadii. The MS proteomics data have been deposited in the ProteomeXchange Consortium with the dataset identifier JPST000395.

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“…Additionally, induction usually takes place in the exponential growth phase of the bacterial culture. In this phase, overexpression of proteins increases the metabolic burden of each cell and proteins which are detrimental for bacterial growth cannot be produced in some cases (Jaishankar and Srivastava, ). These disadvantages could be circumvented by using auto‐inducing expression strategies for recombinant protein production.…”

Section: Introductionmentioning

confidence: 99%

Cell density‐dependent auto‐inducible promoters for expression of recombinant proteins in Pseudomonas putida

Meyers

Furtmann

Gesing

et al. 2019

Microbial Biotechnology

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Summary Inducible promoters such as Plac are of limited usability for industrial protein production with Pseudomonas putida. We therefore utilized cell density‐dependent auto‐inducible promoters for recombinant gene expression in P. putida KT2440 based on the RoxS/RoxR Quorum Sensing (QS) system of the bacterium. To this end, genetic regions upstream of the RoxS/RoxR‐regulated genes ddcA (PRox132) and PP_3332 (PRox306) were inserted into plasmids that mediated the expression of superfolder green fluorescent protein (sfGFP) and surface displayed mCherry, confirming their promoter functionalities. Mutation of the Pribnow box of PRox306 to the σ70 consensus sequence (PRox3061) resulted in a more than threefold increase of sfGFP production. All three promoters caused cell density‐dependent expression, starting transcription at optical densities (OD578) of approximately 1.0 (PRox132, PRox306) or 0.7 (PRox3061) as determined by RT‐qPCR. The QS dependency of PRox306 was further shown by cultivating P. putida in media that had already been used for cultivation and thus contained bacterial signal molecules. The longer P. putida had grown in these media before, the earlier protein expression in freshly inoculated P. putida appeared with PRox306. This confirmed previous findings that a bacterial compound accumulates within the culture and induces protein expression.

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Molecular Basis of Stationary Phase Survival and Applications

Cited by 202 publications

References 89 publications

The Uptake and Release of Amino Acids by Staphylococcus aureus at Mid-Exponential and Stationary Phases and Their Corresponding Responses to Changes in Temperature, pH and Osmolality

The Uptake and Release of Amino Acids by Staphylococcus aureus at Mid-Exponential and Stationary Phases and Their Corresponding Responses to Changes in Temperature, pH and Osmolality

Proteomic Study of the Exponential–Stationary Growth Phase Transition in the Haloarchaea Natrialba magadii and Haloferax volcanii

Cell density‐dependent auto‐inducible promoters for expression of recombinant proteins in Pseudomonas putida

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