Molecular-based environmental risk assessment of three varieties of genetically engineered cows

Xu, Jianzhong; Zhao, Jie; Wang, Jianwu; Zhao, Yaofeng; Zhang, Lei; Chu, Mingxing; Li, Ning

doi:10.1007/s11248-010-9477-3

Cited by 12 publications

(13 citation statements)

References 52 publications

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“…Extracted DNA was quantified by a Tecan infinite 200 microplate reader (Tecan, Switzerland) at 260 and 280 nm (Xu et al 2011). The variable V3 region of bacterial 16S rDNA was amplified with PCR using the primer pair 338F 5′-ACTCCTACGGGAGGCAGCAG-3′ and 518R 5′-ATTACCGCGGCTGCTGG-3′.…”

Section: Pcr-dgge Analysismentioning

confidence: 99%

“…The PCR products were loaded onto 8% (w/v) polyacrylamide gels in 1× Tris-EDTA buffer. The gels were run at 150 V for 420 min and then silver stained (Xu et al 2011). …”

Section: Pcr-dgge Analysismentioning

confidence: 99%

“…Ten clones from each band were randomly selected, and plasmids were isolated with the E.Z.N.A.™ DNA Isolation System (Omega Bio-Tek). An ABI 3730XL DNA sequencer (Applied Biosystems) was used for sequencing using the universal primer pair for the PMD18-T vector, M13F (-40) 5′-GTTTTCCCAGTCACGAC-3′ and M13R (-26) 5′-CAGGAAACAGCTATGAC-3′ (Xu et al 2011).…”

Section: Cloning and Sequencingmentioning

confidence: 99%

“…The method we used is derived from Dice's algorithm and uses the unweighted pair group method with arithmetic averages to construct a similarity matrix (Yang et al 2001;Fromin et al 2002;Boon et al 2002;Smalla et al 2007;Kim et al 2008;Xu et al 2011). The software programs BioEdit 7.0, Phylip 4.0, and MEGA 4.0 were used for cluster analysis.…”

Section: Cluster Analysismentioning

confidence: 99%

“…16S rDNA gene sequences were submitted to the National Center for Biotechnology Information (NCBI) to obtain closely related sequences by BLAST, and all sequences with >97% similarity were chosen for construction of phylogenetic trees (Xing et al 2010;Xu et al 2011). Neighborjoining phylogenetic trees were constructed using MEGA 4.0 (Tamura et al 2007;Xu et al 2011) and assessed using a bootstrap analysis with 1000 replications (Xu et al 2011).…”

Section: Phylogenetic Analysis and Phyla Distributionmentioning

confidence: 99%

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Transgenic milk containing recombinant human lactoferrin modulates the intestinal flora in piglets¹This article is part of a Special Issue entitled Lactoferrin and has undergone the Journal’s usual peer review process.

Zhao

Wang

et al. 2012

Biochem. Cell Biol.

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Lactoferrin (LF) is a beneficial multifunctional protein in milk. The objective of this study was to determine whether bovine transgenic milk containing recombinant human lactoferrin (rhLF) can modulate intestinal flora in the neonatal pig as an animal model for the human infant. We fed 7-day-old piglets (i) ordinary whole milk (OM), (ii) a 1:1 mixture of OM and rhLF milk (MM), or (iii) rhLF milk (LFM). LFM provided better average daily mass gain than OM (P = 0.007). PCR-denaturing gradient gel electrophoresis and 16S rDNA sequencing analysis revealed that the LFM piglets exhibited more diversity of the intestinal flora than the OM group. Except for the colon in the LFM group, an increasing trend in microbial diversity occurred from the duodenum to the colon. Fecal flora was not different across different ages or different treatment groups, but a cluster analysis showed that the fecal flora of OM- and MM-fed piglets had a higher degree of similarity than that of LFM-fed piglets. Based on culture-based bacterial counts of intestinal content samples, concentrations of Salmonella spp. in the colon and of Escherichia coli throughout the intestine were reduced with LFM (P < 0.01). Concentrations of Bifidobacterium spp. in the ileum and of Lactobacillus spp. throughout the intestine were also increased with LFM (P ≤ 0.01). We suggest that rhLF can modulate the intestinal flora in piglets.

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Section: Pcr-dgge Analysismentioning

confidence: 99%

“…The PCR products were loaded onto 8% (w/v) polyacrylamide gels in 1× Tris-EDTA buffer. The gels were run at 150 V for 420 min and then silver stained (Xu et al 2011). …”

Section: Pcr-dgge Analysismentioning

confidence: 99%

Section: Cloning and Sequencingmentioning

confidence: 99%

Section: Cluster Analysismentioning

confidence: 99%

Section: Phylogenetic Analysis and Phyla Distributionmentioning

confidence: 99%

See 3 more Smart Citations

Transgenic milk containing recombinant human lactoferrin modulates the intestinal flora in piglets¹This article is part of a Special Issue entitled Lactoferrin and has undergone the Journal’s usual peer review process.

Zhao

Wang

et al. 2012

Biochem. Cell Biol.

Self Cite

View full text Add to dashboard Cite

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Comparative muscle proteomics/phosphoproteomics analysis provides new insight for the biosafety evaluation of fat-1 transgenic cattle

Xin

Liu

et al. 2017

Transgenic Res

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The biosafety of fat-1 transgenic cattle has been a focus of our studies since the first fat-1 transgenic cow was born. In this study, we used tandem mass tag labeling, TiO enrichment, and nanoscale liquid chromatography coupled with tandem mass spectrometry (nanol LC-MS/MS) to compare proteomic and phosphoproteomic profiling analyses of muscle between fat-1 transgenic cows and wild-type cows. A total of 1555 proteins and 900 phosphorylation sites in 159 phosphoproteins were identified in the profiling assessments, but only four differentially expressed proteins and nine differentially expressed phosphopeptides were detected in fat-1 transgenic cows relative to wild-type cows. Bioinformatics analyses showed that all of the identified proteins and phosphoproteins were mainly related to the metabolic processes of three major nutrients: carbohydrates, lipids, and proteins. All of these differentially expressed proteins might take part in DNA recombination, repair, and regulation of the immune system. In conclusion, most of the identified proteins and phosphoproteins exhibited few changes. Our results provide new insights into the biosafety of fat-1 transgenic cattle.

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Application state of genome-editing tools in cattle

Li,

Wei,

et al. 2024

Livestock Genome Editing Tools

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Molecular-based environmental risk assessment of three varieties of genetically engineered cows

Abstract: The development of animal biotechnology has led to an increase in attention to biosafety issues.

Cited by 12 publications

References 52 publications

Transgenic milk containing recombinant human lactoferrin modulates the intestinal flora in piglets¹This article is part of a Special Issue entitled Lactoferrin and has undergone the Journal’s usual peer review process.

Transgenic milk containing recombinant human lactoferrin modulates the intestinal flora in piglets¹This article is part of a Special Issue entitled Lactoferrin and has undergone the Journal’s usual peer review process.

Comparative muscle proteomics/phosphoproteomics analysis provides new insight for the biosafety evaluation of fat-1 transgenic cattle

Application state of genome-editing tools in cattle

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Molecular-based environmental risk assessment of three varieties of genetically engineered cows

Abstract: The development of animal biotechnology has led to an increase in attention to biosafety issues.

Cited by 12 publications

References 52 publications

Transgenic milk containing recombinant human lactoferrin modulates the intestinal flora in piglets1This article is part of a Special Issue entitled Lactoferrin and has undergone the Journal’s usual peer review process.

Transgenic milk containing recombinant human lactoferrin modulates the intestinal flora in piglets1This article is part of a Special Issue entitled Lactoferrin and has undergone the Journal’s usual peer review process.

Comparative muscle proteomics/phosphoproteomics analysis provides new insight for the biosafety evaluation of fat-1 transgenic cattle

Application state of genome-editing tools in cattle

Contact Info

Product

Resources

About

Transgenic milk containing recombinant human lactoferrin modulates the intestinal flora in piglets¹This article is part of a Special Issue entitled Lactoferrin and has undergone the Journal’s usual peer review process.

Transgenic milk containing recombinant human lactoferrin modulates the intestinal flora in piglets¹This article is part of a Special Issue entitled Lactoferrin and has undergone the Journal’s usual peer review process.