Molecular and serological detection of Theileria equi and Babesia caballi in donkeys (Equus asinus) in Brazil

Machado, Rosangela Zacarias; Toledo, Carmen Zilda Pereira de; Teixeira, Márcia Cristina Alves; André, Marcos Rogério; Freschi, Carla Roberta; Sampaio, Paulo Henrique

doi:10.1016/j.vetpar.2011.11.069

Cited by 42 publications

(35 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The amplifications conditions were the same previously described, except the primers and annealing temperature (Table 1). T. equi and B. caballi DNA samples obtained from naturally infected donkeys (MACHADO et al, 2012) and ultra-pure sterile water were used as positive and negative controls, respectively, in all PCR assays described above. In order to avoid the PCR contamination, DNA extraction, reaction setup, PCR amplification and electrophoresis were performed in separated rooms.…”

Section: Molecular Characterization Of Equid Piroplasmidsmentioning

confidence: 99%

“…In this country, the prevalence among horses ranges from 22 to 100% for T. equi (TENTER & FRIEDHOFF, 1986;PFEIFER BARBOSA et al, 1995;RIBEIRO et al, 1999;HEUCHERT et al, 1999;HEIM et al, 2007;GOLYNSKI et al, 2008;KERBER et al, 2009;BALDANI et al, 2010;SALVAGNI et al, 2010;MACHADO et al, 2012), and from 70 to 90% for B. caballi (PFEIFER BARBOSA et al, 1995;HEIM et al, 2007;KERBER et al, 2009;MACHADO et al, 2012), indicating that both parasites are endemic among horses.…”

Section: Introductionmentioning

confidence: 97%

“…On the other hand, donkeys usually show an asymptomatic form of piroplasmosis, showing a lower parasitaemia when compared to horses (KUMAR et al, 2009;MACHADO et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil

Braga

Costa

Gomes

et al. 2017

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

Equine piroplasmosisis, a tick-borne disease caused by the intra-erythrocytic protozoans Babesia caballi and Theileria equi, has economic importance due to the international trade and the increased movement of horses all over the world. The goal of this study was to evaluate the occurrence of phylogenetic diversity of T. equi and B. caballi genotypes among infected equids from São Luís Island, state of Maranhão, northeastern Brazil. Between December of 2011 and June of 2012, EDTA-blood and serum samples were collected from 139 equids (90 donkeys, 39 horses and 10 mules). From 139 serum samples submitted to ELISA assay, IgG antibodies to T. equi and B. caballi were detected in 19.4% (27/139) and 25.2% (35/139), respectively. Among sampled animals, 21.6% (30/139) and 55.4% (77/139) were positive for cPCR assays for T. equi and B. caballi, based on ema-1 and rap-1 genes, respectively. Overall, the T. equi sequences (n=7) submitted to Maximum Likelihood analysis (based on a 18S rRNA fragment of 1700 bp after alignment) grouped into three main groups, which were subdivided in eight clusters. The present work showed that different genotypes of T. equi and B. caballi circulate among equids in Brazil.Keywords: Babesia caballi, Theileria equi, phylogeny, 18S rRNA. ResumoA piroplasmose equina, uma doença transmitida por carrapatos e causada pelos protozoários intra-eritrocíticos Babesia caballi e Theileria equi, tem importância econômica devido ao comércio internacional e ao aumento do movimento de cavalos em todo o mundo. O objetivo do presente estudo foi mostrar a diversidade filogenética de T. equi e B. caballi infectando cavalos, burros e jumentos na Ilha de São Luís, Estado do Maranhão, Nordeste do Brasil. Entre dezembro de 2011 e junho de 2012, amostras de sangue com EDTA e soro de foram coletadas de 139 equídeos (90 jumentos, 39 cavalos e 10 burros). Dentre as 139 amostras de soro submetidas ao ensaio de ELISA, foram detectados anticorpos IgG contra T. equi e B. caballi em 19,4% (27/139) e 25,2% (35/139), respectivamente. Entre os animais amostrados, 21,6% (30/139) e 55,4% (77/139) foram positivos por meio dos ensaios de cPCR para T. equi e B. caballi, com base nos genes ema-1 e rap-1, respectivamente. No geral, as sequências T. equi (n = 7) submetidas à análise de Máxima Verossimilhança (baseada em um fragmento do 18S rRNA de 1700 pb, após o alinhamento) foram agrupadas em três grupos principais, os quais foram subdivididos em oito grupos. O presente trabalho mostrou que diferentes genótipos de T. equi e B. caballi circulam entre equídeos no Brasil.Palavras-chave: Babesia caballi, Theileria equi, filogenia, 18S rRNA.

show abstract

Section: Molecular Characterization Of Equid Piroplasmidsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil

Braga

Costa

Gomes

et al. 2017

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

show abstract

“…However, no serologic test has been proved to differentiate infections caused by N. caninum and N. hughesi (GONDIM et al, 2009). Donkeys (Equus asinus) are closely related to horses and are affected by several microorganisms that are commonly found in equines, including the protozoa Theileria equi and Babesia caballi (MACHADO et al, 2012). In two publications, donkeys from Italy and Iran were reported to be exposed to Nespora spp.…”

Section: Introductionmentioning

confidence: 99%

Brazilian donkeys (Equus asinus) have a low exposure to Neospora spp.

Galvão

Rezende-Gondim

Chaves

et al. 2015

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

Donkeys (Equus asinus) are closely related to horses and are known to be infected by several equine pathogens. Neospora caninum and Neospora hughesi are protozoan parasites that infect horses, but they were not confirmed in donkeys up to this date. The aim of this study was to evaluate the exposure of donkeys (Equus asinus) to Neospora spp. using tachyzoites of N. caninum as antigen and employing two common serologic methods, IFAT and immunoblot. Sera from 500 donkeys were obtained from 30 municipalities in Bahia state and tested by IFAT. Two of 500 sera were positive for Neospora spp. by IFAT with antibody titers of 100, and recognized a 37kDa antigen in immunoblot. Approximately 22% of the samples showed strong apical reactions and/or incomplete fluorescence, what may cause confusion in the interpretation of IFAT. We concluded that Neospora spp. are possibly of minor importance for Brazilian donkeys. Future studies are necessary to prove that Neospora spp. can naturally infect donkeys.Keywords: Neospora, Equus asinus, donkeys, antibodies. ResumoAsininos (Equus asinus) são próximos filogeneticamente a equinos e podem ser infectados por vários patógenos de cavalos. Neospora caninum e Neospora hughesi são parasitos protozoários que infectam equinos, porém não foram confirmados em asininos até o momento. O objetivo deste estudo foi avaliar a exposição de asininos (Equus asinus) a Neospora spp., usando-se taquizoítos de N. caninum como antígeno e empregando-se duas técnicas sorológicas comuns para esta finalidade, reação de imunofluorescência indireta (RIFI) e immunoblot. Soros de 500 asininos, obtidos em 30 municípios no Estado da Bahia, foram testados por meio da RIFI. Dois dos 500 soros foram positivos para Neospora spp. pela RIFI com títulos de anticorpos de 100, e reconheceram um antígeno de 37kDa no immunoblot. Aproximadamente, 22% das amostras apresentaram fortes reações apicais e/ou fluorescência incompleta, o que pode causar confusão na interpretação da RIFI. Conclui-se que Neospora spp. são, possivelmente, de pouca importância para asininos brasileiros. Estudos posteriores são necessários para provar que Neospora spp. podem causar infecção natural em asininos.

show abstract

“…Clinical symptoms in animals infected with this disease include fever, anemia, dyspnea, jaundice, splenomegaly and hepatomegaly, edema, intravascular hemolysis, hemoglobinuria, mucosa hemorrhage, and finally, death. Piroplasma affect animal health causing loss of appetite, and reducing their work capabilities (10). After suffering an acute infection, animals then enter into a chronic stage with no clinical signs, becoming reservoirs for the microorganisms (11).…”

Section: Introductionmentioning

confidence: 99%

Identificación molecular de hemoparásitos transmitidos por garrapatas en equinos del Noroeste de Colombia

et al. 2017

View full text Add to dashboard Cite

Objetive. To detect and identify Anaplasmataceae agents and piroplasms in equines from the slaughterhouse "La Rinconada" at Rionegro municipality in Antioquia. Materials and Methods. A descriptive cross-sectional study was carried out on equines selected by convenience during a period of 2015. Information about species, sex, age and origin of the animals. Whole blood was collected for DNA extraction procedure, and a PCR targeting a 360bp of Anaplasmataceae 16S ribosomal gene and 450bp of 18S ribosomal gene of Piroplasm were used for detection. PCR amplicons selected were submitted to direct sequencing for identification of hemoparasites through genetic analysis. Results. 135 equine samples from Antioquia, Cordoba y Sucre were analyzed. 78% were horses, 16% were donkeys and 6% were mules. Anaplasmataceae agents were not detected in any sample, meanwhile 13% were positive to piroplasm PCR. Sequence analysis reveals the circulation of Theileria equi (T. equi) in northwestern Colombia. Conclusion. This work presents the first molecular evidence of at least three genotypes of T. equi in equines of northwestern Colombia.Keywords: Anaplasma, Ehrlichia, Theileria, Babesia, PCR, Phylogenetic (Mesh). RESUMENObjetivo. Detectar e identificar agentes de la familia Anaplasmataceae y piroplasmas en equinos colombianos que llegan a la planta de beneficio La Rinconada ubicada en el municipio de Rionegro, Antioquia. Materiales y métodos. Se realizó un estudio descriptivo de corte transversal durante parte del 2015 a equinos seleccionados por conveniencia. Se recopiló información tal como especie, sexo, edad y localidad de procedencia. Se obtuvieron muestras de sangre para extracción de ADN, y se amplificó un fragmento de 360pb del gen 16S ribosomal de Anaplasmataceae y un fragmento de 450 pb del gen 18S ribosomal de piroplasmas para detección de hemoparásitos. Amplicones de PCR fueron sometidos a secuenciación para identificación de los hemoparásitos a través de análisis genético. Resultados. Se analizaron 135 equinos provenientes de los departamentos de Antioquia, Córdoba y Sucre. Un 78% eran caballos, un 16% eran asnos y un 6% eran mulas. El 100% de los animales fueron negativos para agentes de la familia Anaplasmataceae y un 13% fueron positivos para piroplasmas. Se identificó por secuenciación la circulación de Theileria equi (T. equi) en la zona norte de Colombia. Conclusión. Se presenta la primera evidencia molecular de al menos tres genotipos de T. equi infectando equinos del norte del país.

show abstract

Molecular and serological detection of Theileria equi and Babesia caballi in donkeys (Equus asinus) in Brazil

Cited by 42 publications

References 27 publications

Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil

Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil

Brazilian donkeys (Equus asinus) have a low exposure to Neospora spp.

Identificación molecular de hemoparásitos transmitidos por garrapatas en equinos del Noroeste de Colombia

Contact Info

Product

Resources

About