Molecular and phylogenetic characterisation of Cryptosporidium from birds

Morgan, U.M.; Monis, Paul; Xiao, Lihua; Limor, Josef; Sulaiman, Irshad M.; Raidal, Shane R.; O’Donoghue, P. J.; Gasser, Robin B.; Murray, Alan V.; Fayer, Ronald; Blagburn, Byron L.; Lal, Altaf A.; Thompson, R.C.A.

doi:10.1016/s0020-7519(00)00164-8

Cited by 178 publications

(116 citation statements)

References 26 publications

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“…The eluted DNA was dissolved in 15 l ultra-pure water. Previously described nested PCR protocols for the characterisation of the HSP-70 gene (Morgan et al, 2001) and for the 18S rDNA gene (Ryan et al, 2003) were used in this study. Amplification products were subsequently visualized on 1.5% agarose gels with ethidium bromide.…”

Section: Methodsmentioning

confidence: 99%

The prevalence and molecular characterisation of Cryptosporidium spp. in small ruminants in Zambia

Goma

Geurden

Siwila

et al. 2007

Small Ruminant Research

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Although Cryptosporidium spp. have been associated with diarrhea and mortality in sheep and goats, this is the first study to estimate the prevalence of Cryptosporidium spp. in small ruminants in Southern Africa. Between August 2003 and February 2004, a cross-sectional study with single random sampling was carried out in three Zambian provinces: Central, Southern and Lusaka province. Faecal samples from lambs and goat kids less than 3 months of age were collected and examined using the TechLab Cryptosporidium ELISA. Faecal samples of 257 animals were collected: 152 lambs on 18 farms and 105 goat kids on 13 farms. The prevalence of Cryptosporidium spp. in lambs and goat kids was 12.5% and 4.8%, respectively and 30% of the sheep and goat farms had at least one positive animal at the time of the visit. A subset of the positive samples was withheld for molecular characterisation. Based on sequences obtained after amplification of the 70 kDa heat shock protein and the Cryptosporidium 18SrRNA gene, C. parvum was identified in all but one of the animals. In this lamb C. suis was identified. The results of the present study indicate that the animal prevalence in lambs and goats kids is rather low. However, the identification of C. parvum illustrates that small ruminants might be potential reservoir for human infection in Zambia.

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Section: Methodsmentioning

confidence: 99%

The prevalence and molecular characterisation of Cryptosporidium spp. in small ruminants in Zambia

Goma

Geurden

Siwila

et al. 2007

Small Ruminant Research

View full text Add to dashboard Cite

show abstract

“…It has previously been suggested that C. meleagridis may be C. parvum (Champliaud, et al, 1998, Sreter and, however molecular analysis of US, Australian and European isolates at the small subunit ribosomal RNA (SSU rRNA), heat shock protein 70 (HSP70), Cryptosporidium oocyst wall protein (COWP), actin and glycoprotein 60 (GP60) loci as well as the Cryptosporidium double stranded RNA virus have demonstrated the genetic and biological uniqueness of C. meleagridis , Morgan, et al, 2001.…”

Section: Cryptosporidium Meleagridis Slavin 1955mentioning

confidence: 99%

“…Cryptosporidium baileyi has been analysed at a number of loci including the SSU rRNA, HSP70, COWP and actin loci and has been shown to be genetically distinct , Morgan, et al, 2001, Egyed, et al, 2002. Cryptosporidium baileyi is most closely related to avian genotype I and II (see 1.5.1).…”

Section: Cryptosporidium Baileyi Current Upton and Haynes 1986mentioning

confidence: 99%

“…Cryptosporidium galli appears to be associated with clinical disease and high mortality , Pavlásek, 1999, Morgan, et al, 2001, Pavlásek, 2001. Histopathology of infected finches demonstrated necrosis and hyperplasia of proventricular glandular epithelial cells associated with large numbers of Cryptosporidium oocysts attached to the surface of glandular epithelial cells (Morgan, et al, 2001).…”

Section: Cryptosporidium Galli Pavlasek 1999mentioning

confidence: 99%

“…Histopathology of infected finches demonstrated necrosis and hyperplasia of proventricular glandular epithelial cells associated with large numbers of Cryptosporidium oocysts attached to the surface of glandular epithelial cells (Morgan, et al, 2001). …”

Section: Cryptosporidium Galli Pavlasek 1999mentioning

confidence: 99%

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