Molecular and phenotypic analysis of Moraxella spp. associated with infectious bovine keratoconjunctivitis in Uruguay

Sosa, Vanessa; Zunino, Pablo

doi:10.1016/j.tvjl.2012.01.011

Cited by 9 publications

(12 citation statements)

References 5 publications

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“…Moreover, these results are in accordance with our previous phenotypic analyses which reported that all these strains were β-hemolytic [7]. According to these results and to previous reports, Moraxella spp.…”

Section: Discussionsupporting

confidence: 93%

“…bovis has been considered the main aetiological agent of IBK for decades [24]. Recently an increasing number of studies have demonstrated the presence of M. bovoculi associated to IBK cases [6][7][8]. It has been established that this is the Moraxella spp.…”

Section: Discussionmentioning

confidence: 99%

“…All isolates had been previously characterized and extensively identified in our laboratory. All of them exhibited β-hemolytic activity when grown on blood agar plates [7,19].…”

Section: Bacterial Strains Media and Growth Conditionsmentioning

confidence: 99%

“…However, more recently, other species belonging to the genus like Moraxella bovoculi have also been associated to IBK cases in USA, Brazil and Uruguay so far [1,[6][7][8].…”

Section: Introductionmentioning

confidence: 99%

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Virulence genes in Moraxella spp. isolates from infectious bovine keratoconjunctivitis cases

Sosa

Umpiérrez

Acquistapace

et al. 2015

J Infect Dev Ctries

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Introduction: Infectious bovine keratoconjunctivitis (IBK) is an important ocular disease which affects cattle worldwide. To advance towards IBK effective prevention and treatment strategies, it is important to define the distribution and genetic diversity of potential virulence factors present in M. bovis and M. bovoculi. The objective of this work was to identify and to analyze Moraxella spp. potential virulence factor genes in a collection of clinical isolates. Methodology: The presence and diversity of virulence factors in a collection of Moraxella spp. strains isolated since 1983 to 2009 in Uruguay was analyzed by PCR using primers for partial amplification of tolC, omp79, plb, fur and mbxA. The selection criterion of these genes was based on the fact that they encode virulence factors which could be present and conserved within strains, an important issue for the development of vaccines. Results: Differences in PCR amplification were observed within tolC (84%), omp79 (80%), plb (76%) and fur (44%) in M. bovis strains, whereas mbxA was amplified in all M. bovis and M. bovoculi strains. Regarding genetic diversity, the tolC nucleotide sequences were the less diverse within all M. bovis and mbxA were the less diverse within all M. bovis and M. bovoculi strains. Conclusions: PCR amplifications suggest the occurrence of differences between both Moraxella species, related to evaluated genes within Moraxella spp. strains and suggests that both species may have different pathogenic attributes. MbxA and the outer membrane protein TolC might be considered for future studies to develop new vaccines against IBK.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

“…All isolates had been previously characterized and extensively identified in our laboratory. All of them exhibited β-hemolytic activity when grown on blood agar plates [7,19].…”

Section: Bacterial Strains Media and Growth Conditionsmentioning

confidence: 99%

“…However, more recently, other species belonging to the genus like Moraxella bovoculi have also been associated to IBK cases in USA, Brazil and Uruguay so far [1,[6][7][8].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Virulence genes in Moraxella spp. isolates from infectious bovine keratoconjunctivitis cases

Sosa

Umpiérrez

Acquistapace

et al. 2015

J Infect Dev Ctries

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“…However, the recently characterized species Moraxella bovoculi, has been recovered from corneal ulcers of beef and dairy calves in USA [2] and in Uruguay [3].…”

Section: Introductionmentioning

confidence: 99%

Diversity of Moraxella spp. strains recovered from infectious bovine keratoconjunctivitis cases in Uruguay

Sosa

Zunino

2013

J Infect Dev Ctries

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Background: Infectious bovine keratoconjunctivitis (IBK) is the most common ocular disease that affects cattle throughout the world and it has a very significant economic impact. IBK is caused by members of the genus Moraxella and therapeutic and preventive measures have shown limited success. Vaccines, most of them chemically inactivated bacterins, generally induce a limited protection. Methodology: In this study, the genetic diversity of Uruguayan clinical Moraxella bovis and Moraxella bovoculi isolates was assessed by RAPD-PCR, ERIC-PCR and BOX-PCR fingerprinting. Also, antibiotic resistance of the Moraxella spp. isolates was assessed utilizing the disk diffusion method. Results: When interspecific molecular diversity was assessed, different bands patterns were observed even within a single outbreak of IBK, showing the coexistence of different genotypes of Moraxella spp. The high genetic diversity within M. bovis and M. bovoculi isolates did not permit to correlate isolates DNA fingerprints with geographical origins, dates or even with both different Moraxella species. Antibiotics resistance patterns showed significant differences between M. bovis and M. bovoculi. Conclusions: This is the first study of diversity that includes M. bovis and M. bovoculi associated to IBK cases. Genetic diversity did not allow to correlate DNA fingerprints of the isolates with geographical origins, isolation dates or even both different Moraxella species. Antibiotics resistance patterns showed differences between M. bovis and M. bovoculi. This remarkable variation within isolates could explain the partial protection induced by commercial vaccines. All these findings could be important for the design of prevention or treatment strategies against IBK.

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Genomic characterization of Moraxella bovis and Moraxella bovoculi Uruguayan strains isolated from calves with infectious bovine keratoconjunctivitis

Bilbao,

Acquistapace,

Umpiérrez

et al. 2024

Revista Argentina de Microbiología

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Molecular and phenotypic analysis of Moraxella spp. associated with infectious bovine keratoconjunctivitis in Uruguay

Cited by 9 publications

References 5 publications

Virulence genes in Moraxella spp. isolates from infectious bovine keratoconjunctivitis cases

Virulence genes in Moraxella spp. isolates from infectious bovine keratoconjunctivitis cases

Diversity of Moraxella spp. strains recovered from infectious bovine keratoconjunctivitis cases in Uruguay

Genomic characterization of Moraxella bovis and Moraxella bovoculi Uruguayan strains isolated from calves with infectious bovine keratoconjunctivitis

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