Molecular and cellular analysis of rP1.B in the rat hypothalamus: In situ hybridization and immunohistochemistry of a new P-domain neuropeptide

Probst, Joseph Christopher; Skutella, Thomas; Müller-Schmid, Angelika; Jirikowski, Gustav F.; Hoffmann, Werner

doi:10.1016/0169-328x(95)00137-h

Cited by 40 publications

(36 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cerebellar expression pattern is perfectly in line with the previous RT-PCR analysis from murine brain regions [11]. Synthesis of TFF3 in neurons is also in agreement with its synthesis in magnocellular neurons of the human, rat and porcine hypothalamus and release by the neural lobe of the pituitary [12,14,15]. …”

Section: Discussionsupporting

confidence: 73%

“…Weak Tff3 expression has also been reported to occur in the rat amygdala [12] and injections of synthetic Tff3 into this brain region showed fear modulating effects [13]. Furthermore, TFF3 is also synthesized in the rat and human hypothalamus [12,14] where it localizes to oxytocin-producing neurons in the paraventricular and supraoptic nuclei [14]. Immunoelectron microscopy revealed that TFF3 and oxytocin are co-localizing within the same secretory vesicles in the neural (posterior) lobe of the porcine pituitary [15].…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, Tff3 expression in the murine cerebellum is developmentally regulated with a maximum between P15 and P20 [11]. Weak Tff3 expression has also been reported to occur in the rat amygdala [12] and injections of synthetic Tff3 into this brain region showed fear modulating effects [13]. Furthermore, TFF3 is also synthesized in the rat and human hypothalamus [12,14] where it localizes to oxytocin-producing neurons in the paraventricular and supraoptic nuclei [14].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Tff3 is Expressed in Neurons and Microglial Cells

Stellmacher

Znalesniak

et al. 2014

Cell Physiol Biochem

Self Cite

View full text Add to dashboard Cite

Background/Aims: The trefoil factor family (TFF) peptide TFF3 is typically secreted by mucous epithelia, but is also expressed in the immune system and the brain. It was the aim of this study to determine the cerebral cell types which express Tff3. Methods: Primary cultures from rat embryonic or neonatal cerebral cortex and hippocampus, respectively, were studied by means of RT-PCR and immunofluorescence. Moreover, Tff3 expression was localized by immunocytochemistry in sections of adult rat cerebellum. Results: Tff3 transcripts were detectable in neural cultures of both the cortex and the hippocampus as well as in glial cell-enriched cultures. Tff3 peptide co-localized with Map2 indicating an expression in neurons in vitro. The neuronal expression was confirmed by immunofluorescence studies of adult rat cerebellum. Furthermore, Tff3 peptide showed also a clear co-localization with Iba-1 in vitro typical of activated microglial cells. Conclusion: The neuronal expression of Tff3 is in line with a function of a typical neuropeptide influencing, e.g., fear, memory, depression and motoric skills. The expression in activated microglial cells, which is demonstrated here for the first time, points towards a possible function for Tff3 in immune reactions in the CNS. This opens a plethora of additional possible functions for Tff3 including synaptic plasticity and cognition as well as during neuroinflammatory diseases and psychiatric disorders.

show abstract

Section: Discussionsupporting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Tff3 is Expressed in Neurons and Microglial Cells

Stellmacher

Znalesniak

et al. 2014

Cell Physiol Biochem

Self Cite

View full text Add to dashboard Cite

show abstract

“…Abundant TFF3 synthesis has also been noted in goblet cells of the conjunctiva, contributing, together with TFF1, to the eye's tear fluid and protective mucus [10]. In addition to these epithelia-related functions of TFFs an intriguing new function appears to arise from several reports pointing to Tff1 expression in perinatal rat hippocampus [11] and Tff3 synthesis in the rat and human hypothalamus [12,13]. The presence of the latter exclusively in oxytocinergic neurons but not in vasopressinergic neurons suggested its role as neuropeptide acting together with oxytocin along the hypothalamo-pituitary axis [14].…”

Section: Hubert Löwenheimmentioning

confidence: 99%

Lack of Tff3 Peptide Results in Hearing Impairment and Accelerated Presbyacusis

Lubka

Müller²,

Baus-Lončar³

et al. 2008

Cell Physiol Biochem

Self Cite

View full text Add to dashboard Cite

Tff peptides are secreted mainly by the gastrointestinal epithelial cells and their primary role is maintaining normal structure and function of mucous epithelia. Ongoing studies on their expression pattern have disclosed other sites of their synthesis thus revealing additional physiological functions in the organism. Here we present new data about Tff3 expression in the cochlea of the rodent inner ear. On the basis of RT-PCR we describe the presence of Tff3 transcripts in both, a mouse cDNA library isolated from whole cochleae from postnatal days 3-15 (P3-P15), and also in cochlear tissue. By using a riboprobe for the fragment containing exon 1, 2 and 3 of Tff3, in situ hybridization, localized Tff3 signals in neurons of spiral ganglion and vestibular organ. We did not observe any abnormalities in the middle ear of Tff3 knock-out mice, neither did histological examination of the inner ear indicate any gross morphological changes in the cochlea. However, ABR (auditory evoked brain stem responses) audiograms revealed that the Tff3 knock-out animals show an accelerated presbyacusis and a hearing loss of about 15 dB at low frequencies increasing to 25 dB loss at higher frequencies. These findings suggest that Tff3 could play a role in neurosensory signaling. Further studies are needed to clarify this new function in the auditory system.

show abstract

“…TFF3 is expressed constitutively in the gastrointestinal tract, primarily in the duodenum and colon (36), as well as in other tissues such as brain (37), uterus (38), and breast (39). Trefoil peptides have been shown to be overexpressed within gastrointestinal mucosa affected by chronic ulcerative and inflammatory lesions, suggesting a possible role in mucosal healing (40,41).…”

mentioning

confidence: 99%

Intestinal trefoil factor controls the expression of the adenomatous polyposis coli–catenin and the E-cadherin–catenin complexes in human colon carcinoma cells

Efstathiou

Noda

Rowan

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

140

View full text Add to dashboard Cite

Intestinal trefoil factor 3 (TFF3) is a member of the trefoil family of peptides, small molecules constitutively expressed in epithelial tissues, including the gastrointestinal tract. TFF3 has been shown to promote migration of intestinal epithelial cells in vitro and to enhance mucosal healing and epithelial restitution in vivo. In this study, we evaluated the effect of recombinant TFF3 (rTFF3) stimulation on the expression and cellular localization of the epithelial (E)-cadherin-catenin complex, a prime mediator of Ca 2؉ dependent cell-cell adhesion, and the adenomatous polyposis coli (APC)-catenin complex in HT29, HCT116, and SW480 colorectal carcinoma cell lines. Stimulation by rTFF3 (10 ؊9 M and 10 ؊8 M) for 20-24 hr led to cell detachment and to a reduction in intercellular adhesion in HT29 and HCT116 cells. In both cell lines, E-cadherin expression was down-regulated. The expression of APC, ␣-catenin and ␤-catenin also was decreased in HT29 cells, with a translocation of APC into the nucleus. No change in either cell adhesion or in the expression of E-cadherin, the catenins, and APC was detected in SW480 cells. In addition, TFF3 induced DNA fragmentation and morphological changes characteristic of apoptosis in HT29. Tyrphostin, a competitive inhibitor of protein tyrosine kinases, inhibited the effects of TFF3. Our results indicate that by perturbing the complexes between E-cadherin, ␤-catenin, and associated proteins, TFF3 may modulate epithelial cell adhesion, migration, and survival.

show abstract

Molecular and cellular analysis of rP1.B in the rat hypothalamus: In situ hybridization and immunohistochemistry of a new P-domain neuropeptide

Cited by 40 publications

References 24 publications

Tff3 is Expressed in Neurons and Microglial Cells

Tff3 is Expressed in Neurons and Microglial Cells

Lack of Tff3 Peptide Results in Hearing Impairment and Accelerated Presbyacusis

Intestinal trefoil factor controls the expression of the adenomatous polyposis coli–catenin and the E-cadherin–catenin complexes in human colon carcinoma cells

Contact Info

Product

Resources

About