Molecular Analysis ofSalmonellaEnteritidis and Typhimurium Clinical and Food Isolates by Pulsed-Field Gel Electrophoresis in Bogotá, Colombia

Abstract

In 1997, the Microbiology Group of the Colombian Instituto Nacional de Salud set up a surveillance program with the National Public Health Laboratories to monitor the principal etiological agents responsible for acute diarrheal disease. The main goal of this study was to determine the XbaI DNA digestion patterns of clinical and food Salmonella spp. isolates recovered in Bogotá from 1997 to 2002, and related them to the susceptibility patterns to antimicrobial agents. Two hundred and twenty-four Salmonella spp. isolates were studied, 153 (63%) S. Enteritidis and 71 (37%) S. Typhimurium. Pulsed-field gel electrophoresis (PFGE) was done using the XbaI restriction enzyme and Salmonella Braenderup H9812 as the molecular weight marker. For S. Enteritidis, pattern S. En0001 was found to be prevalent in 127 (83%) isolates, 78 (61%) susceptible to the antimicrobial agents tested. For S. Typhimurium, pattern S. Typ0001 was predominant in 18 (25%) isolates with different antimicrobial resistance profiles. Patterns S. En0001 and S. Typ0001 prevailed monthly during the 6 years of the study. Data collected demonstrate that there was a dominant pattern of S. Enteritidis and S. Typhimurium circulating in clinical and food isolates in Bogotá, Colombia.

Characterization of antimicrobial resistance in Salmonella enterica food and animal isolates from Colombia: identification of a qnrB19-mediated quinolone resistance marker in two novel serovars

“…No Enteritidis serovars were recovered. The most common serovars implicated in human salmonellosis in Colombia are Enteritidis and Typhimurium (Munoz et al , 2006; Wiesner et al , 2006). However, serovars identified in this study have occasionally been implicated in salmonellosis outbreaks worldwide (Lehmacher et al , 1995; Jones et al , 2004; Gupta et al , 2007; Lang, 2008).…”

Caracterización por electroforesis de campo pulsado de aislamientos de Salmonella Typhimurium recuperados en el programa de vigilancia de enfermedad diarreica aguda en Colombia, 1997-2004

“…Este trabajo determinó la relación genética en los 468 aislamientos colombianos de S. Typhimurium, observándose que, debido al número de patrones electroforéticos encontrados, el serotipo Typhimurium presenta una considerable diversidad genética, lo que coincide con los hallazgos de otros autores que han utilizado la PFGE y describen aislamientos muy heterogéneos recuperados en una misma región (21,22). Esta diversidad, representada por los subtipos de PFGE, probablemente muestra que las infecciones proceden de diferentes fuentes (10).…”

“…Typhimurium es un organismo considerablemente diverso en comparación con el serotipo S. Enteritidis, que es altamente clonal (21,27). Por ello, la detección de aislamientos estrechamente relacionados en diferentes áreas geográficas podría ser una señal de diseminación o aparición de grupos clonales que han incrementado su virulencia (28).…”

“…La numeración de los patrones se continuó de acuerdo a la secuencia de la base de datos descrita por Wiesner et al (21).…”

The Establishment of the Colombian Integrated Program for Antimicrobial Resistance Surveillance (COIPARS): A Pilot Project on Poultry Farms, Slaughterhouses and Retail Market

“…and Shigella spp. (Ovalle et al, 2003;Moreno et al, 2004;Agudelo et al, 2006;Wiesner et al, 2006;INS, 2010).…”