2006
DOI: 10.1637/7525-030706r.1
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: Molecular analysis of 15 Brazilian infectious bronchitis virus (IBV) isolates, obtained from clinical outbreaks of the disease in chickens (broilers or layers) in the state of Minas Gerais (Brazil) between 1972 and 1989, is reported. Using the N protein gene as target, IBVs were analyzed by reverse transcription-polymerase chain reaction/restriction fragment length polymorphism (RT-PCR/RFLP) with the restriction enzymes AvaII, HphI, Sau96I, and Tsp509I and cDNA sequencing. Results obtained from those isolates … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

1
11
0
2

Year Published

2008
2008
2018
2018

Publication Types

Select...
6
1

Relationship

0
7

Authors

Journals

citations
Cited by 17 publications
(14 citation statements)
references
References 43 publications
1
11
0
2
Order By: Relevance
“…In a study by Abreu et al (2006), 15 Brazilian IBV isolates generated three different RFLP patterns based on the N gene which they classified into six groups based on partial N gene sequencing, revealing less conservation in the N gene of Brazilian isolates despite the fact the N gene is considered highly conserved (Huang et al 2004;Shieh et al 2004;Williams et al 1992). Park et al (2005) illustrated multiple divergences between Korean and non-Korean IBV isolates based on N protein gene analysis, indicating that the N gene sequence analysis could be used as a useful method to differentiate Korean isolates from non-Korean strains.…”
Section: Discussionmentioning
confidence: 98%
See 1 more Smart Citation
“…In a study by Abreu et al (2006), 15 Brazilian IBV isolates generated three different RFLP patterns based on the N gene which they classified into six groups based on partial N gene sequencing, revealing less conservation in the N gene of Brazilian isolates despite the fact the N gene is considered highly conserved (Huang et al 2004;Shieh et al 2004;Williams et al 1992). Park et al (2005) illustrated multiple divergences between Korean and non-Korean IBV isolates based on N protein gene analysis, indicating that the N gene sequence analysis could be used as a useful method to differentiate Korean isolates from non-Korean strains.…”
Section: Discussionmentioning
confidence: 98%
“…Due to the limitations of conventional methods in the identification of IBVs, reverse transcription and polymerase chain reaction (RT-PCR) accompanied with restriction fragment length polymorphism (RFLP) mainly based on S1 gene of IBVs has recently been employed for the rapid molecular identification of IBV isolates worldwide (Abreu et al 2006;Kwon et al 1993;Mardani et al 2006b;Wang and Khan 2000); however, because of the highly variable nature of the S1 gene of the IBV genome and occurring recombination events in this region, it can be difficult to find suitable primers for amplifying this gene for molecular analysis when a rapid response is required for an IBV outbreak (Jia et al 1995;Kottier et al 1995;Lee and Jackwood 2000). The 3′ UTR of the IBV genome has a highly variable region which has been used to differentiate different IBV strains in recent studies (Hewson et al 2009;Mardani et al 2006a;Williams et al 1993).…”
Section: Introductionmentioning
confidence: 99%
“…Além disso, a partir de 2000 e com base na análise das sequências de nucleotídeos do gene S1 de isolados de campo do VBI foram identificados, no Brasil, genótipos diferentes daqueles em que são classificadas as estirpes americanas, europeias, asiáticas e australianas do VBI (Figura 1) (BALESTRIN et al, 2014;CHACÓN et al, 2011;FELIPPE et al, 2010;FRAGA et al, 2013;MONTASSIER et al, 2006MONTASSIER et al, , 2012VILLARREAL et al, 2007VILLARREAL et al, , 2010. Além disso, foram também identificadas estirpes variantes brasileiras do VBI com relação à sequência de nucleotídeos do gene da nucleoproteína (N) (ABREU et al, 2006).…”
Section: As Primeiras Variantes Do Vbi Foram Isoladas No Início Dos Aunclassified
“…Cinco genótipos diferentes do vírus no Brasil foram descritos por MONTASSIER et al (2008): M, A, B, C e D (Tabela 1 e Figura 1), confirmando a grande variabilidade genética no pool de IBV no campo, já sugerida por outros autores (DIFABIO et al, 2000;ABREU et al, 2006;VILLARREAL et al, 2007a,b). Entretanto, os estudos sobre a distribuição dos sorotipos de IBV no Brasil ainda são escassos, gerando dificuldades em se obter uma descrição filogeográfica mais completa e precisa.…”
Section: Epidemiologiaunclassified