Moisture-Dependent Physical and Physiological Properties of Accelerated Aged Pea (<i>Pisum sativum</i> L.) Seeds

Abstract

The present study was carried out to determine the physical as well as physiological properties of three fresh pea seed lots (cv. Arkel) with moisture content and germination percentage varying from 14.94% to 28.04% dry basis and 80% to 60% respectively. This variation in moisture content and physiological parameters was obtained using accelerated aging (40C and 100% RH). The geometric (spatial dimensions, sphericity and surface area), gravimetric (terminal velocity, true density, test weight, bulk density and porosity), frictional (angle of repose, coefficient of static friction), mechanical (compressive strength) and physiological parameters (seedling dry weight, seedling length, vigour indices, electrical conductivity and root growth parameters) were determined for the selected seed lots. The effect of moisture content on seed lots was significant (R 2  0.947) on physical and physiological properties of seed lots. This study may help in designing seed priming prototype suitable for pea seeds.

SlHyPRP1 and DEA1, the multiple stress responsive eight-cysteine motif family genes of tomato (Solanum lycopersicum L.) are expressed tissue specifically, localize and interact at cytoplasm and plasma membrane in vivo

“…The novel finding from our in vivo study is the strong interaction between SlHyPRP1 and DEA1 in cytoplasm and plasma membrane. As we observed from our subcellular localization study, both proteins localized to cytoplasm and plasma membrane, both belong to 8CM superfamily proteins associated with abiotic and biotic stress responses (Gujjar et al 2018). Along with multiple abiotic stress responses, SlHyPRP1 is also sensitive to pathogen attack by degrading cell wall bound pathogens during biotic stress (Takahashi 2010;Li et al 2016;Yang et al 2018).…”

“…We can infer from our localization results that, the HyPRPs are putative cell wall structural proteins (Li et al 2016). In order to perform the osmo-protectant activity during stress (Gujjar et al 2018) as well as for cell wall thickening and ROS accumulation in response to pathogen attack (Yang et al 2018), they might be localized at plasma membrane and cytoplasm. DEA1 is an 8CM family protein primarily expressed in taproot and stem involved in biotic and abiotic stress responses (Eulgem et al 2000).…”

“…SlHyPRP1 and DEA1 are known as putative regulators of different abiotic and biotic stress responses in plants (Li et al 2016;Gujjar et al 2018). Despite their significance as putative multi-stress responsive genes, very little information is available on their tissue specificity.…”

Comparative analysis of the physicochemical composition of pea cultivars

“…AA was conducted by exposing 50 seeds to approximately 100 % relative humidity created in a chamber at 42 °C for 96 h. If the difference in germination between samples before and after seed AA was higher than 75 %, the method was considered efficient. It has been established that pea seed must have 75 % minimum germination to maintain physical and genetic purity (Mahawar et al, 2018). All analyses were conducted in duplicate.…”