1997
DOI: 10.1021/bi971933j
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Abstract: Melittin is a cationic hemolytic peptide isolated from the European honey bee, Apis mellifera. Since the association of the peptide in the membrane is linked with its physiological effects, a detailed understanding of the interaction of melittin with membranes is crucial. We have investigated the interaction of melittin with membranes of varying surface charge in the context of recent studies which show that the presence of negatively charged lipids in the membrane inhibits membrane lysis by melittin. The sole… Show more

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Cited by 143 publications
(212 citation statements)
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“…Solvents used were of spectroscopic grade. The concentration of melittin in aqueous solution was calculated from its molar absorption coefficient ( ) of 5570 M )1 cm )1 at 280 nm (Ghosh et al 1997). The purity of the detergents was checked by measuring their CMC values and comparing with literature CMC.…”
Section: Methodsmentioning
confidence: 99%
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“…Solvents used were of spectroscopic grade. The concentration of melittin in aqueous solution was calculated from its molar absorption coefficient ( ) of 5570 M )1 cm )1 at 280 nm (Ghosh et al 1997). The purity of the detergents was checked by measuring their CMC values and comparing with literature CMC.…”
Section: Methodsmentioning
confidence: 99%
“…We have previously monitored the microenvironment experienced by the sole tryptophan of melittin when bound to membranes, utilizing the wavelength-selective fluorescence approach. Our results show that the tryptophan residue is located in a motionally restricted region in the membrane (Chattopadhyay and Rukmini 1993;Ghosh et al 1997) and the tryptophan environment is modulated by the surface charge of the membrane, which could be related to the difference in the lytic activity of the peptide observed in membranes of varying charge (Ghosh et al 1997). In this paper, we have utilized micelles of various charge types to monitor the effect of varying surface charge on the conformation and dynamics of micelle-bound melittin.…”
Section: Introductionmentioning
confidence: 98%
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