Modulation of Streptococcus mutans virulence by dental adhesives containing anti-caries agents

André, Carolina Bosso; Rosalen, Pedro Luiz; Galvão, Lívia Câmara de Carvalho; Fronza, Bruna Marin; Ambrosano, Gláucia María Bovi; Ferracane, Jack L.; Giannini, Marcelo

doi:10.1016/j.dental.2017.07.006

Cited by 23 publications

(12 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This mechanism of action has been previously reported in the literature; for example, the commercial product Gluma 2 Bond that contains glutaraldehyde has been demonstrated that requires at least 24 h. to produce any antibacterial effect against S. mutans ( André et al, 2015 ). In the same study, chlorhexidine-containing adhesive Peak Universal Bond killed only strict anaerobic microorganisms also after 24 h. It was previously demonstrated the effect of the antibacterial properties and dentin bond strength of experimental adhesives formed with the addition of tt -farnesol into 2 commercial products, Clearfil S3 Bond Plus which is a 7th generation (single step self-etch) and OptiBond S, a 5th generation (two-step etch-and-rinse) adhesive systems ( André et al, 2017 ). In the mentioned study, there was no reduction of bacterial viability measured in CFU for any of the experimental adhesives tested.…”

Section: Discussionmentioning

confidence: 87%

“…Thus, the introduction of tt -farnesol into restorative materials is justified due its demonstrated antibacterial properties against cariogenic pathogens. In recent literature, tt -farnesol was incorporated into dental restorative materials such as a glass ionomer ( de Castilho et al, 2019 ) and into a self-etch (5th generation) and etch-and-rinse (7th generation) dental adhesives ( André et al, 2017 ). Although the addition of agents such as epigallocatechin-3-gallate have been recently introduced ( Silva et al, 2020 ) into universal adhesive systems (8th generation) demonstrating an antimicrobial potential against S. mutans , the incorporation of tt -farnesol into universal adhesive systems has not yet been described in the literature.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bioactive Dental Adhesive System With tt-Farnesol: Effects on Dental Biofilm and Bonding Properties

Rio

Sartori

Tomblin

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Background: Composite dental restorations are commonly used to restore cavitated carious lesions. Unfortunately, the main reason for failure is the development of secondary caries adjacent to the restoration. To improve the long-term survival of restorations, antibacterial agents have been added into dental materials. In this study, we assessed the antibacterial and bonding capacity of a commercial universal dental adhesive incorporated with the antibacterial agent tt-farnesol creating 3 experimental adhesives: 0.38% (v/v), 1.90% (v/v), and 3.80% (v/v), plus a control (no incorporation of tt-farnesol). Methods: The antibacterial activity was evaluated by assessing colony-forming units (CFU), biofilm dry weight (DW) and production of extracellular insoluble polysaccharides (EIP) at day 2, 3, and 5 of biofilm growth post surface treatment on the surface of composite disks. The effect of tt-farnesol on the chemical and bonding capacity of the adhesive system was assessed via pH analysis, degree of conversion (DC), and microtensile bond strengths to human dentin in both self-etch and etch-andrinse application modes. A qualitative analysis of the effects of tt-farnesol on biofilm formation was evaluated using scanning electron microscopy (SEM). The sealing capacity of all adhesive systems tested was evaluated using confocal laser scanning microscopy (CLSM). Results: The 3.80% (v/v) experimental adhesive exhibited the lowest CFU count and lowest production of EIP at day 5. DW and pH values did no exhibit statistical differences among all tested groups. Bond strengths and DC decreased with the incorporation of the antibacterial agent into the adhesive system regardless of the concentration of tt-farnesol. Conclusion: The incorporation of tt-farnesol into the adhesive system significantly reduced bacterial viability and production of EIP; however, the bonding properties of the experimental dental adhesives were altered.

show abstract

Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Bioactive Dental Adhesive System With tt-Farnesol: Effects on Dental Biofilm and Bonding Properties

Rio

Sartori

Tomblin

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Pinocembrin and apigenin were quantified in the EP extract obtained from La Araucanía. These polyphenols have been related to antimicrobial activities against S. mutans [19, 20].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and Antibiofilm Activity against Streptococcus mutans of Individual and Mixtures of the Main Polyphenolic Compounds Found in Chilean Propolis

Veloz

Alvear

Salazar

2019

BioMed Research International

View full text Add to dashboard Cite

Dental caries is multifactorial disease and an important health problem worldwide. Streptococcus mutans is considered as a major cariogenic agent in oral cavity. This bacteria can synthetize soluble and insoluble glucans from sucrose by glucosyltransferases enzymes and generate stable biofilm on the tooth surface. Biological properties of Chilean propolis have been described and it includes antimicrobial, antifungal, and antibiofilm activities. The main goal of this study was to quantify the concentrations of main flavonoids presents in Chilean propolis and compare some biological properties such as antimicrobial and antibiofilm activity of individual compounds and the mixture of this compounds, against S. mutans cultures. Chilean propolis was studied and some polyphenols present in this extract were quantified by HPLC-DAD using commercial standards of apigenin, quercetin, pinocembrin, and caffeic acid phenethyl ester (CAPE). MIC for antimicrobial activity was determined by serial dilution method and biofilm thickness on S. mutans was quantified by confocal microscopy. Pinocembrin, apigenin, quercetin, and caffeic acid phenethyl ester (CAPE) are the most abundant compounds in Chilean propolis. These polyphenols have strong antimicrobial and antibiofilm potential at low concentrations. However, pinocembrin and apigenin have a greater contribution to this action. The effect of polyphenols on S. mutans is produced by a combination of mechanisms to decrease bacterial growth and affect biofilm proliferation due to changes in their architecture.

show abstract

“…Although it does not always dominate human dental plaque, in the presence of sucrose S mutans can assemble an insoluble exopolysaccharide (EPS). This EPS acts as a supportive framework for diffusion within the oral biofilm structure (Koo et al, 2013;Kim et al, 2015;Andre et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Streptococcus mutans Biofilm Formation and Virulence by Lactobacillus plantarum K41 Isolated From Traditional Sichuan Pickles

Zhang

Liu

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

Among cariogenic microbes, Streptococcus mutans is considered a major etiological pathogen of dental caries. Lactobacilli strains have been promoted as possible probiotic agents against S. mutans, although the inhibitory effect of Lactobacilli on caries has not yet been properly addressed. The objective of this study was to screen Lactobacillus strains found in traditional Sichuan pickles and to evaluate their antagonistic properties against S. mutans in vitro and in vivo. In the current study, we analyzed 54 Lactobacillus strains isolated from pickles and found that strain L. plantarum K41 showed the highest inhibitory effect on S. mutans growth as well as on the formation of exopolysaccharides (EPS) and biofilm in vitro. Scanning electron microscopy (SEM) and confocal laser scanning microscope (CLSM) revealed the reduction of both EPS and of the networklike structure in S. mutans biofilm when these bacteria were co-cultured with strain L. plantarum K41. Furthermore, when rats were treated with strain L. plantarum K41, there was a significant reduction in the incidence and severity of dental caries. Due to K41's origin in a high salinity environment, it showed a high tolerance to acids and salts. This may give this strain an advantage in harsh oral conditions. Results showed that L. plantarum K41 isolated from traditional Sichuan pickles effectively inhibited S. mutans biofilm formation and thus possesses a potential inhibitory effect on dental caries in vivo.

show abstract

Modulation of Streptococcus mutans virulence by dental adhesives containing anti-caries agents

Cited by 23 publications

References 29 publications

Bioactive Dental Adhesive System With tt-Farnesol: Effects on Dental Biofilm and Bonding Properties

Bioactive Dental Adhesive System With tt-Farnesol: Effects on Dental Biofilm and Bonding Properties

Antimicrobial and Antibiofilm Activity against Streptococcus mutans of Individual and Mixtures of the Main Polyphenolic Compounds Found in Chilean Propolis

Inhibition of Streptococcus mutans Biofilm Formation and Virulence by Lactobacillus plantarum K41 Isolated From Traditional Sichuan Pickles

Contact Info

Product

Resources

About