Modulation of<i>in vitro</i>rumen biohydrogenation by<i>Cistus ladanifer</i>tannins compared with other tannin sources

Costa, Mónica; Alves, Susana P.; Cabo, Ângelo; Guerreiro, Olinda; Stilwell, George; Dentinho, M. T.; Bessa, Rui J. B.

doi:10.1002/jsfa.7777

Cited by 30 publications

(24 citation statements)

References 41 publications

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“…How does the presence of other forage compounds (carbohydrates and lipids) effect CT-protein interactions and precipitation. There have been a number of studies conducted on how CTs affect rumen bacteria and protozoa populations (McSweeney et al, 2001;Sivakumaran et al, 2004;Smith et al, 2005;Benchaar et al, 2008;Tan et al, 2011;Piñeiro-Vázquez et al, 2015) and biohydrogenation (Khiaosa-Ard et al, 2009;Vasta et al, 2009;Costa et al, 2017). Some research has been conducted on how carbohydrates present in the digesta may affect CT-protein complex formation and utilization Liang et al, 2013;Barros et al, 2014).…”

Section: The Path Forward: Questions To Addressmentioning

confidence: 99%

Activity, Purification, and Analysis of Condensed Tannins: Current State of Affairs and Future Endeavors

Zeller

2019

Crop Science

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As a class of plant polyphenolic compounds contained in some forages (i.e., sainfoin [Onobrychis viciifolia Scop.], big trefoil [Lotus pedunculatus Cav.], birdsfoot trefoil [Lotus corniculatus L.]), condensed tannins (CTs), also referred to as proanthocyanidins (PAs or PACs), exhibit a variety of biological effects on ruminants. The potential positive impact of CTs on the agricultural industry stems from their ability to modulate proteolysis during forage conservation and ruminal digestion, to prevent bloat, to reduce intestinal parasite burdens, to abate methane and ammonia emissions from ruminants, and to inhibit the activity of soil‐nitrifying bacteria. How CTs exert these effects on ruminants focuses on the interaction of CTs with proteins. The structure‐activity relationship in CT–protein interaction is not well understood but is known to be dependent on the structure and properties of both the CT and the protein. The purpose of this perspective is fivefold. First, we provide the reader with a better understanding of the structural diversity of CTs present in plant material and enable the reader to appreciate that not all CTs are the same. Second, we provide examples of how CT structural diversity affects the interaction of CTs with the protein, which, in turn, dictates the biological response from the animal. Third, we describe the hurdles in obtaining highly pure and well‐characterized CTs from natural sources for use in studies to attempt to elucidate how CTs impart each biological effect. We then describe improved and emerging techniques for CT analysis and, finally, we conclude this perspective with questions to address in future investigations and forward a list of recommendations for CT researchers to follow.

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Section: The Path Forward: Questions To Addressmentioning

confidence: 99%

Activity, Purification, and Analysis of Condensed Tannins: Current State of Affairs and Future Endeavors

Zeller

2019

Crop Science

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“…The utilization of CT extracts from various origins to modulate the ruminal BH as a way to increase the rumen outflow of healthy FA, such as t11-18:1, c9, t11-18:2 and dietary PUFA has been the subject of several studies [19,[27][28][29][30]. Although some results have shown the potential of this strategy to improve the FA profile of ruminant edible fat, overall, the effects of CT extracts on ruminal metabolism including fermentation [31][32][33][34][35] and BH [5][6][7]12,13,19,27,29,30] are inconsistent. The diversity the effects of CT extracts on ruminal fermentation and BH may be due to several factors, such as CT composition and concentration, basal substrate, and experimental conditions [19,33,34].…”

Section: Discussionmentioning

confidence: 99%

“…Some in vitro studies have suggested that CTs may be efficient in inhibiting the last step of BH, decreasing the 18:0 formation and leading to t11-18:1 accumulation [5,6,11]. However, other studies reported a general inhibition or stimulation of BH without the inhibition of its last step [7,12,13].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, there is increasing evidence that the effect of tannins on ruminal BH depends on the features of the basal diet [19]. The ability of C. ladanifer CT extract to modulate the in vitro ruminal BH was only demonstrated using an oil-supplemented high-forage substrate [7,12]. Thus, the present in vitro experiment was designed to evaluate the effect of increasing doses of CT extract from C. ladanifer on rumen fermentation and BH when an oil-supplemented high-concentrate substrate is used.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Increasing Doses of Condensed Tannins Extract from Cistus ladanifer L. on In Vitro Ruminal Fermentation and Biohydrogenation

Guerreiro

Alves

Costa³

et al. 2021

Animals

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Cistus ladanifer (rockrose) is a perennial shrub quite abundant in the Mediterranean region, and it is a rich source in secondary compounds such as condensed tannins (CTs). Condensed tannins from C. ladanifer were able to change the ruminal biohydrogenation (BH), increasing the t11–18:1 and c9,t11–18:2 production. However, the adequate conditions of the C. ladanifer CTs used to optimize the production of t11–18:1 and c9,t11–18:2 is not yet known. Thus, we tested the effect of increasing the doses of C. ladanifer CT extract (0, 25, 50, 75 and 100 g/kg dry matter (DM)) on in vitro rumen BH. Five in vitro batch incubations replicates were conducted using an oil supplemented high-concentrate substrate, incubated for 24 h with 6 mL of buffered ruminal fluid. Volatile fatty acids (VFAs) and long chain fatty acids (FA) were analyzed at 0 h and 24 h, and BH of c9–18:1, c9, c12–18:2 and c9, c12, c15–18:3, and BH products yield were computed. Increasing doses of C. ladanifer CTs led to a moderate linear decrease (p < 0.001) of the VFA production (a reduction of 27% with the highest dose compared to control). The disappearance of c9–18:1 and c9,c12–18:2 as well as the production of t11–18:1 and c9, t11:18:2 was not affected by increasing doses of C. ladanifer CTs, and only the disappearance of c9, c12, c15–18:3 suffered a mild linear decrease (a reduction of 24% with the highest dose compared to control). Nevertheless, increasing the C. ladanifer CT dose led to a strong depression of microbial odd and branched fatty acids and of dimethyl acetals production (less than 65% with the highest dose compared to control), which indicates that microbial growth was more inhibited than fermentative and biohydrogenation activities, in a possible adaptative response of microbial population to stress induced to CTs and polyunsaturated fatty acids. The ability of C. ladanifer to modulate the ruminal BH was not verified in the current in vitro experimental conditions, emphasizing the inconsistent BH response to CTs and highlighting the need to continue seeking the optimal conditions for using CTs to improve the fatty acid profile of ruminant fat.

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“…However, the lower feed consumption of BB60 could mean that the total TP intake does not reach those of the AP treatments. Several studies have demonstrated the inhibitory action of dietary polyphenols on ruminal biohydrogenation of PUFA, without detrimental effects on milk yield and composition, due to interference with microbial flora [52][53][54][55]. These effects have also been observed in sheep with small amounts in the diet of by-products rich in TP [56,57].…”

Section: Milk Fatty Acid Profilementioning

confidence: 99%

Composition, Mineral and Fatty Acid Profiles of Milk from Goats Fed with Different Proportions of Broccoli and Artichoke Plant By-Products

Monllor

Romero

Atzori

et al. 2020

Foods

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In the Mediterranean region, artichoke and broccoli are major crops with a high amount of by-products that can be used as alternative feedstuffs for ruminants, lowering feed costs and enhancing milk sustainability while reducing the environmental impact of dairy production. However, nutritional quality of milk needs to be assured under these production conditions and an optimal inclusion ratio of silages should be determined. This work aimed to evaluate the effect of three inclusion levels (25%, 40%, and 60%) of these silages (artichoke plant, AP, and broccoli by-product, BB) in goat diets on milk yield, composition, and mineral and fatty profiles. Treatments with 60% inclusion of AP and BB presented the lowest milk yield. No differences were found on the milk mineral profile. Inclusion of AP in the animals’ diet improved the milk lipid profile from the point of view of human health (AI, TI) compared to BB due to a lower saturated fatty acid content (C12:0, C14:0, and C16:0) and a higher concentration of polyunsaturated fatty acids (PUFA), especially vaccenic acid (C18:1 trans11) and rumenic acid (CLA cis9, trans11), without any differences with the control treatment.

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Modulation ofin vitrorumen biohydrogenation byCistus ladanifertannins compared with other tannin sources

Abstract: Condensed tannins from GS and, to a lesser extent, from CL stimulate the first steps of BH, without a clear inhibition of 18:0 production. © 2016 Society of Chemical Industry.

Cited by 30 publications

References 41 publications

Activity, Purification, and Analysis of Condensed Tannins: Current State of Affairs and Future Endeavors

Activity, Purification, and Analysis of Condensed Tannins: Current State of Affairs and Future Endeavors

Effects of Increasing Doses of Condensed Tannins Extract from Cistus ladanifer L. on In Vitro Ruminal Fermentation and Biohydrogenation

Composition, Mineral and Fatty Acid Profiles of Milk from Goats Fed with Different Proportions of Broccoli and Artichoke Plant By-Products

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