Modulation of Corneal Wound Healing After Excimer Laser Keratomileusis Using Topical Mitomycin C and Steroids

The PALM Technique: histological findings of masked phototherapeutic keratectomy on rabbit corneas

“…The extrusion of electron dense fibro-granular material into the descemet's membrane is also a common finding observed in a number of previous studies [20,21,27-29] and has been attributed to acoustic shock waves by irradiation [24] or the distraction of the epithelial integrity after trauma [29]. The presence of this finding in only one of our specimens may be related to the homogenous and smooth removal of the corneal epithelium with the rotating brush.…”

The PALM Technique: histological findings of masked phototherapeutic keratectomy on rabbit corneas

“…The extrusion of electron dense fibro-granular material into the descemet's membrane is also a common finding observed in a number of previous studies [20,21,27-29] and has been attributed to acoustic shock waves by irradiation [24] or the distraction of the epithelial integrity after trauma [29]. The presence of this finding in only one of our specimens may be related to the homogenous and smooth removal of the corneal epithelium with the rotating brush.…”

Reversal of fibrosis by TGF-β3 in a 3D in vitro model

“…Although multiple studies have demonstrated that scarring can be blunted by inhibiting the fibrosis pathway at the time of wounding (Blalock et al 2012; Buhren et al 2009; Huxlin et al 2013; Jester et al 1997; Sriram et al 2013; Talamo et al 1991), the current studies, to the best of our knowledge, are the first to demonstrate that an established model of human fibrosis can be reversed. Our previous studies on TGF-β3 (Karamichos et al 2011) have shown the prevention of scarring in vitro; however, in the present study, we attempted to reverse an established scar-like ECM.…”

Mitomycin C Alters Corneal Stromal Wound Healing and Corneal Haze in Rabbits After Argon-Fluoride Excimer Laser Photorefractive Keratectomy

“…By applying DTAF to stain the stromal collagen immediately after PRK, observation by fluorescence microscope of unstained sections of the corneal stroma allowed assessment of the amount of new collagen production in the wound bed (19). Under observation by fluorescence microscope, we found that mitomycin C 0.02% eye drops applied twice daily for 5 days after PRK could inhibit stromal thickness regression and new tissue deposition at Week 4.…”

The PALM Technique: histological findings of masked phototherapeutic keratectomy on rabbit corneas

“…The extrusion of electron dense fibro-granular material into the descemet's membrane is also a common finding observed in a number of previous studies [20,21,27-29] and has been attributed to acoustic shock waves by irradiation [24] or the distraction of the epithelial integrity after trauma [29]. The presence of this finding in only one of our specimens may be related to the homogenous and smooth removal of the corneal epithelium with the rotating brush.…”

Reversal of fibrosis by TGF-β3 in a 3D in vitro model

“…Although multiple studies have demonstrated that scarring can be blunted by inhibiting the fibrosis pathway at the time of wounding (Blalock et al 2012; Buhren et al 2009; Huxlin et al 2013; Jester et al 1997; Sriram et al 2013; Talamo et al 1991), the current studies, to the best of our knowledge, are the first to demonstrate that an established model of human fibrosis can be reversed. Our previous studies on TGF-β3 (Karamichos et al 2011) have shown the prevention of scarring in vitro; however, in the present study, we attempted to reverse an established scar-like ECM.…”

Mitomycin C Alters Corneal Stromal Wound Healing and Corneal Haze in Rabbits After Argon-Fluoride Excimer Laser Photorefractive Keratectomy

“…By applying DTAF to stain the stromal collagen immediately after PRK, observation by fluorescence microscope of unstained sections of the corneal stroma allowed assessment of the amount of new collagen production in the wound bed (19). Under observation by fluorescence microscope, we found that mitomycin C 0.02% eye drops applied twice daily for 5 days after PRK could inhibit stromal thickness regression and new tissue deposition at Week 4.…”

The PALM Technique: histological findings of masked phototherapeutic keratectomy on rabbit corneas

“…The extrusion of electron dense fibro-granular material into the descemet's membrane is also a common finding observed in a number of previous studies [20,21,27-29] and has been attributed to acoustic shock waves by irradiation [24] or the distraction of the epithelial integrity after trauma [29]. The presence of this finding in only one of our specimens may be related to the homogenous and smooth removal of the corneal epithelium with the rotating brush.…”

Reversal of fibrosis by TGF-β3 in a 3D in vitro model

“…Although multiple studies have demonstrated that scarring can be blunted by inhibiting the fibrosis pathway at the time of wounding (Blalock et al 2012; Buhren et al 2009; Huxlin et al 2013; Jester et al 1997; Sriram et al 2013; Talamo et al 1991), the current studies, to the best of our knowledge, are the first to demonstrate that an established model of human fibrosis can be reversed. Our previous studies on TGF-β3 (Karamichos et al 2011) have shown the prevention of scarring in vitro; however, in the present study, we attempted to reverse an established scar-like ECM.…”

Mitomycin C Alters Corneal Stromal Wound Healing and Corneal Haze in Rabbits After Argon-Fluoride Excimer Laser Photorefractive Keratectomy

“…By applying DTAF to stain the stromal collagen immediately after PRK, observation by fluorescence microscope of unstained sections of the corneal stroma allowed assessment of the amount of new collagen production in the wound bed (19). Under observation by fluorescence microscope, we found that mitomycin C 0.02% eye drops applied twice daily for 5 days after PRK could inhibit stromal thickness regression and new tissue deposition at Week 4.…”