Modulation of aflatoxin B1 activated protein kinase C by phenolic compounds

Mistry, K.J.; Krishna, M. Bala; Bhattacharya, R.K.

doi:10.1016/s0304-3835(97)00337-6

Cited by 22 publications

(12 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Natural flavonoids have the ability to reduce the enzyme activity consequent to AFB 1 treatment (Mistry et al, 1997;Abdel-Wahhab and Aly, 2005); accordingly, the suppression of protein kinase C activity by flavonoid compounds could by a way to control zen toxicity. Moreover, the increase in GSH activity induced by radish extract may be another way to prevent zen carcinogenicity through the increase of zen-glutathione conjugates and consequently decreased the ability of hepatic microsomes to metabolize zen and prevent the formation of DNA adduct and prevent its toxic effects.…”

Section: Discussionmentioning

confidence: 99%

Tunisian radish extract (Raphanus sativus) enhances the antioxidant status and protects against oxidative stress induced by zearalenone in Balb/c mice

Salah-Abbès¹,

Abbès²,

Ouanes

et al. 2007

J of Applied Toxicology

View full text Add to dashboard Cite

Radish (Raphanus sativus) is a food plant known worldwide. From antiquity it has been used in folk medicine as a natural drug against many toxicants. Zearalenone (zen) is a non-steroidal estrogenic mycotoxin present in corn and food mixture for farm animals and it is hepatotoxic, hematotoxic, immunotoxic, nephrotoxic and genotoxic. The objectives of the present study were to assess the biological activity of radish extract and to evaluate the protective role of radish extract against the toxicity of zen in female Balb/c mice. Animals were divided into seven groups and treated orally for 10 days as follows: a control, an olive oil group, groups treated with radish extract alone (5, 10 and 15 mg kg(-1) b.w.), a group treated with zen (40 mg kg(-1) b.w.) and a group treated with zen plus the lowest dose of radish extract. The results indicate that radish extract improved the antioxidant status and had no significant effects on hematological and biochemical parameters tested or histology of the liver and kidney. Treatment with zen results in a significant increase in ALT, AST, ALP, BILT, BILD, CRE accompanied with significant changes in most of hematological parameters and the antioxidant enzyme activities, co-treatment of zen and the radish extract results in a significant reestablishment of hematological, serum biochemical parameters, and the histology of the liver and kidney. These findings suggest that radish extract is safe and can be overcome or, at least, significantly diminish zen effects.

show abstract

Section: Discussionmentioning

confidence: 99%

Tunisian radish extract (Raphanus sativus) enhances the antioxidant status and protects against oxidative stress induced by zearalenone in Balb/c mice

Salah-Abbès¹,

Abbès²,

Ouanes

et al. 2007

J of Applied Toxicology

View full text Add to dashboard Cite

show abstract

“…Rompelberg et al (1996) found that eugenol does not modify the unscheduled DNA synthesis in hepatocytes exposed to aflatoxin B 1 . Natural phenolics such as those found in S. aromaticum oil have the ability to reduce the enzyme activity consequent to aflatoxin B 1 treatment (Mistry et al, 1997), and the suppression of protein kinase C activity by phenolic compounds could thus control the aflatoxin carcinogenicity. Moreover, the increasie in glutathione S-transferase activity induced by eugenol may be another way of preventing aflatoxin carcinogenicity through the increase of aflatoxin-glutathione conjugates and consequently the decreased ability of hepatic microsomes to metabolize aflatoxin, preventing the formation of epoxides and DNA adduct formation.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant property ofNigella sativa (black cumin) andSyzygium aromaticum (clove) in rats during aflatoxicosis

2005

View full text Add to dashboard Cite

Aflatoxins, a group of closely related, extremely toxic mycotoxins produced by Aspergillus flavus and A. parasiticus, can occur as natural contaminants of foods and feeds. Aflatoxins have been shown to be hepatotoxic, carcinogenic, mutagenic and teratogenic to different animal species. Nigella sativa (black cumin) and Syzygium aromaticum (clove) oil are used for the treatment of inflammatory diseases and have antioxidant properties. The aim of this study was to investigate the ability of these volatile oils to scavenge free radicals generated during aflatoxicosis. Sixty male rats were divided into six treatment groups, including a control group, and the groups were treated for 30 days with Nigella sativa and Syzygium aromaticum oils with or without aflatoxin. Blood samples were collected at the end of the experimental period for haematological and biochemical analysis. The results indicated that exposure to aflatoxins resulted in haematological and biochemical changes typical for aflatoxicosis. Treatment with Nigella sativa and Syzygium aromaticum oil of rats fed an aflatoxin-contaminated diet resulted in significant protection against aflatoxicosis. Moreover, Nigella sativa oil was found to be more effective than Syzygium aromaticum oil in restoring the parameters that were altered by aflatoxin in rats.

show abstract

“…1). Ellagic acid, a phenolic acid with two aromatic rings, has been reported to be inhibitory towards particulate PKC isolated from rats after carcinogen treatment (IC 50 ¼ 55 mM) [Mistry et al, 1997], and also towards rat brain PKC (IC 50 ¼ 8 mM) [Wang et al, 1998]. Hence, a more complex structure than a single aromatic ring with substituents seems to be required for PKC inhibition.…”

Section: Phenolic Acids and Derivativesmentioning

confidence: 99%

“…More than 500 natural and synthetic flavonoids have been shown to inhibit the growth of cancer cell lines [Ló pezLázaro et al, 2002]. The connection between cancer and PKC has stimulated a search for new potential PKC modulators among flavonoids and other natural compounds and products [Ferriola et al, 1989;Agullo et al, 1997;Nick et al, 1995;Mistry et al, 1997;Zhou et al, 1998]. Multiple mechanisms of action have been identified for the antiproliferative and cancer preventive effects of flavonoids [Birt et al, 2001].…”

Section: Introductionmentioning

confidence: 99%

Screening of natural compounds and their derivatives as potential protein kinase C inhibitors

Tammela

Ekokoski

Garcı́a-Horsman

et al. 2004

Drug Development Research

View full text Add to dashboard Cite

Protein kinase C (PKC) isozymes are important signal transducers in a number of cellular responses that makes PKC inhibition a topical target for drug discovery. In this study, a set of natural compounds and their derivatives (43 in total) were screened to establish their potential inhibitory effects on PKC, exploiting kinase activity and [ 3 H]-phorbol ester binding assays. Statistical evaluation of the assays yielded signal-to-background, signal-to-noise, and Z 0 values of 16.1, 11.3, and 0.62, respectively, for the kinase activity assay and 47.4, 15.9, and 0.73 for the binding assay, demonstrating their suitability for screening. Of the compounds investigated, the most potent PKC inhibitor was (À)-epigallocatechin gallate (EGCG), which had an IC 50 of 4.8 mM. In addition, (À)-epicatechin gallate, dodecyl gallate, and the flavonoids myricetin, quercetin, rhamnetin, luteolin, isorhamnetin, and kaempferol were effective while naringin and scopoletin demonstrated negligible effects. None of the compounds was able to significantly inhibit the binding of phorbol ester to the regulatory domain of PKCa. The highest inhibition, 59%, was observed in the case of EGCG at a concentration of 150 mM. Taken together, nine PKC inhibitors were identified, none of which was able to compete with the binding of phorbol ester to PKCa, suggesting that the mechanism of PKC inhibition could be a result of binding to the catalytic domain of PKC. Drug Dev

show abstract

Modulation of aflatoxin B1 activated protein kinase C by phenolic compounds

Cited by 22 publications

References 40 publications

Tunisian radish extract (Raphanus sativus) enhances the antioxidant status and protects against oxidative stress induced by zearalenone in Balb/c mice

Tunisian radish extract (Raphanus sativus) enhances the antioxidant status and protects against oxidative stress induced by zearalenone in Balb/c mice

Antioxidant property ofNigella sativa (black cumin) andSyzygium aromaticum (clove) in rats during aflatoxicosis

Screening of natural compounds and their derivatives as potential protein kinase C inhibitors

Contact Info

Product

Resources

About