Modularity of methylotrophy, revisited

Chistoserdova, Ludmila

doi:10.1111/j.1462-2920.2011.02464.x

Cited by 350 publications

(464 citation statements)

References 125 publications

(228 reference statements)

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“…The aerobic nature of the denitrification enzymes and low enzyme activities agree with prior data on relatively low expression of the respective genes/proteins (14). Assays for methanol dehydrogenase were also negative, as previously reported for wild-type M. mobilis JLW8 (20,21), in each of the xoxF mutants, including the mutant with enhanced growth on methanol.…”

Section: Mutant Growth Phenotypessupporting

confidence: 76%

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Insights into Denitrification in Methylotenera mobilis from Denitrification Pathway and Methanol Metabolism Mutants

et al. 2013

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We investigated phenotypes of mutants of Methylotenera mobilis JLW8 with lesions in genes predicted to encode functions of the denitrification pathway, as well as mutants with mutations in methanol dehydrogenase-like structural genes xoxF1 and xoxF2, in order to obtain insights into denitrification and methanol metabolism by this bacterium. By monitoring the accumulation of nitrous oxide, we demonstrate that a periplasmic nitrate reductase, NAD(P)-linked and copper-containing nitrite reductases, and a nitric oxide reductase are involved in the denitrification pathway and that the pathway must be operational in aerobic conditions. However, only the assimilatory branch of the denitrification pathway was essential for growth on methanol in nitrate-supplemented medium. Mutants with mutations in each of the two xoxF genes maintained their ability to grow on methanol, but not the double XoxF mutant, suggesting that XoxF proteins act as methanol dehydrogenase enzymes in M. mobilis JLW8. Reduced levels of nitrous oxide accumulated by the XoxF mutants compared to the wild type suggest that these enzymes must be capable of donating electrons for denitrification.

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Section: Mutant Growth Phenotypessupporting

confidence: 76%

“…We also mutated a gene predicted to encode a nitric oxide dioxygenase (Nod; mmol_1063) and each of two xoxF genes (mmol_1770 and mmol_2048) that encode homologs of the large subunit of methanol dehydrogenase (11,14,19,20). In addition, a double mutant lacking both XoxF proteins was generated.…”

Section: Mutant Growth Phenotypesmentioning

confidence: 99%

Insights into Denitrification in Methylotenera mobilis from Denitrification Pathway and Methanol Metabolism Mutants

et al. 2013

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“…The numbers of mch-and fae-like genes were greater suggesting a broader diversity of detected methylotroph taxa. mch-and fae-like genes also occur in non-methylotrophs (Chistoserdova et al, 2009;Chistoserdova, 2011). Thus, the high diversity of novel mch and fae genotypes may be additionally caused by codetected non-methylotrophs.…”

Section: Genotype Diversity Of Mxaf Mch and Faementioning

confidence: 99%

“…A homolog (mdh2) exists in Burkholderiaceae, but mdh2 is not as widely distributed within methylotrophs as mxaF (Kalyuzhnaya et al, 2008). xoxF, a similar gene, occurs in all known methylotroph genomes (Chistoserdova, 2011). Its gene product XoxF oxidizes in vitro methanol (Schmidt et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Methanol oxidation by temperate soils and environmental determinants of associated methylotrophs

et al. 2012

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The role of soil methylotrophs in methanol exchange with the atmosphere has been widely overlooked. Methanol can be derived from plant polymers and be consumed by soil microbial communities. In the current study, methanol-utilizing methylotrophs of 14 aerated soils were examined to resolve their comparative diversities and capacities to utilize ambient concentrations of methanol. Abundances of cultivable methylotrophs ranged from 10 6 -10 8 g soilDW. Methanol dissimilation was measured based on conversion of supplemented 14 C-methanol, and occurred at concentrations down to 0.002 lmol methanol g soilDW À 1. Tested soils exhibited specific affinities to methanol (a 0 s ¼ 0.01 d À 1 ) that were similar to those of other environments suggesting that methylotrophs with similar affinities were present. Two deep-branching alphaproteobacterial genotypes of mch responded to the addition of ambient concentrations of methanol (p0.6 lmol methanol g soilDW) in one of these soils. Methylotroph community structures were assessed by amplicon pyrosequencing of genes of mono carbon metabolism (mxaF, mch and fae). Alphaproteobacteria-affiliated genotypes were predominant in all investigated soils, and the occurrence of novel genotypes indicated a hitherto unveiled diversity of methylotrophs. Correlations between vegetation type, soil pH and methylotroph community structure suggested that plant-methylotroph interactions were determinative for soil methylotrophs.

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“…Methanotrophic bacteria is a subdivision of the methylotrophs, which are a diverse group of organisms able to utilize different compounds containing a single-carbon atom e.g., methanol, methylated amines, halomethanes [3], [4].…”

Section: Introductionmentioning

confidence: 99%

Physiological Features during the Copper Switch in Methylococcus Capsulatus (Bath)

Khalifa¹

2013

IJBBB

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Abstract-Copper-to-biomass ratio is an important factor that controls the biosynthesis and catalytic activity of the two forms (particulate or soluble) of methane monooxygenase enzyme in Methylococcus capsulatus (Bath). Under growth conditions of high copper-to-biomass ratio, the particulate enzyme is expressed while the soluble enzyme is up-regulated when this ratio is low. It is of interest to explore physiological features of cells in response to copper addition. To this end, M. capsulatus was grown on Nitrate Mineral Salts medium in a bioreactor and 30 µM copper was added at a dilution rate of 3.3 ml min -1 at steady state. Samples were collected at different time points and assayed for soluble methane monooxygenase (sMMO) expression using the naphthalene assay, SDS-PAGE of total cell protein and copper measurements. The results showed that the activity of sMMO was gradually decreased upon gradual copper addition until it became fully inactive after 4 hours of adding copper. Furthermore, the intracellular, extracellular and copper associated with biomass increased by adding copper over the time tested. Eleven bands from the SDS-PAGE gel of sMMO-expressing cells and pMMO-expressing cells, were analyzed by mass spectrometry. The results highlighted that 128 proteins were identified from 792 polypeptides detected from the eleven bands analysed. Typically, 506 polypeptides from sMMO-expressing cells (corresponding to 87 proteins) and 236 from pMMO-expressing cells (corresponding to 41) were identified. Collectively, the results confirmed that copper negatively regulates sMMO and induces pMMO in M. capsulatus.

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Modularity of methylotrophy, revisited

Cited by 350 publications

References 125 publications

Insights into Denitrification in Methylotenera mobilis from Denitrification Pathway and Methanol Metabolism Mutants

Insights into Denitrification in Methylotenera mobilis from Denitrification Pathway and Methanol Metabolism Mutants

Methanol oxidation by temperate soils and environmental determinants of associated methylotrophs

Physiological Features during the Copper Switch in Methylococcus Capsulatus (Bath)

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