Modified laser speckle imaging method with improved spatial resolution

Cheng, Haiying; Luo, Qingming; Zeng, Shaoqun; Chen, Shangbin; Cen, Jian; Gong, Hui

doi:10.1117/1.1578089

Cited by 231 publications

(179 citation statements)

References 12 publications

(23 reference statements)

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“…Currently, several approaches have been implemented to measure flow velocities in small animal vessels, e.g. fast confocal laser scanning microscopy (Lucitti et al, 2007), optical coherence tomography (Larina et al, 2009), and laser speckle imaging (Cheng et al, 2003). However, those imaging based methods provided data characterized by good temporal but low spatial resolution, and a high level of spatial resolution is a key factor to understand the local changes of blood flow in the vessel.…”

Section: Blood Flow Measurement In Live Zebrafish Embryosmentioning

confidence: 99%

Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy

Shi

Teo

Pan

et al. 2009

Developmental Dynamics

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Zebrafish and Drosophila are animal models widely used in developmental biology. High-resolution microscopy and live imaging techniques have allowed the investigation of biological processes down to the cellular level in these models. Here, using fluorescence correlation spectroscopy (FCS), we show that even processes on a molecular level can be studied in these embryos. The two animal models provide different advantages and challenges. We first characterize their autofluorescence pattern and determine usable penetration depth for FCS especially in the case of zebrafish, where tissue thickness is an issue. Next, the applicability of FCS to study molecular processes is shown by the determination of blood flow velocities with high spatial resolution and the determination of diffusion coefficients of cytosolic and membrane-bound enhanced green fluorescent protein-labeled proteins in different cell types. This work provides an approach to study molecular processes in vivo and opens up the possibility to relate these molecular processes to developmental biology questions. Developmental Dynamics 238:3156-3167,

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Section: Blood Flow Measurement In Live Zebrafish Embryosmentioning

confidence: 99%

Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy

Shi

Teo

Pan

et al. 2009

Developmental Dynamics

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“…In general, subcutaneous blood flow, which is a dynamically fluctuating biological variable and has substantial spatial heterogeneity, is invasively monitored by performing open surgery to reveal vasculature [1,5,7,8,11]. However, it was successfully monitored in real time in the present study without open surgery by applying the PCTOC, which clearly reveals vasculature in deep tissue layers.…”

Section: Discussionmentioning

confidence: 81%

“…Various optical and image processing methods have been proposed for enhancing the contrast of LSCI in subcutaneous blood flow measurements [1,[8][9][10][11][12][13]. However, LSCI still suffers from low image contrast, although the limitation of laser penetration depth in tissue due to the light scattering property has been partially addressed.…”

Section: Introductionmentioning

confidence: 99%

Contrast Enhancement of Laser Speckle Contrast Image in Deep Vasculature by Reduction of Tissue Scattering

Son¹,

Lee²,

Jung³

2013

Journal of the Optical Society of Korea

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Various methods have been proposed for enhancing the contrast of laser speckle contrast image (LSCI) in subcutaneous blood flow measurements. However, the LSCI still suffers from low image contrast due to tissue turbidity. Herein, a physicochemical tissue optical clearing (PCTOC) method was employed to enhance the contrast of LSCI. Ex vivo and in vivo experiments were performed with porcine skin samples and male ICR mice, respectively. The ex vivo LSCIs were obtained before and 90 min after the application of the PCTOC and in vivo LSCIs were obtained for 60 min after the application of the PCTOC. In order to obtain the skin recovery images, saline was applied for 30 min after the application of the PCTOC was completed. The visible appearance of the tubing under ex vivo samples and the in vivo vasculature gradually enhanced over time. The LSCI increased as a function of time after the application of the PCTOC in both ex vivo and in vivo experiments, and properly recovered to initial conditions after the application of saline in the in vivo experiment. The LSCI combined with the PCTOC was greatly enhanced even in deep vasculature. It is expected that similar results will be obtained in in vivo human studies.

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“…Recently, this laser speckle imaging (LSI) method has been developed into a digital, quasi real-time technique for the mapping of blood flow [6,7]. LSI has been used to monitor noninvasively blood flow and perfusion dynamics in the brain [1,[8][9][10][11][12], retina [5,13], and skin [14][15][16][17]. We have employed LSI to monitor blood flow dynamics during photodynamic therapy [16] and have observed marked changes in the measured speckle flow index (SFI) values which is proportional to the blood flow velocity.…”

Section: Introductionmentioning

confidence: 99%

Impact of velocity distribution assumption on simplified laser speckle imaging equation

Ramos-Garcı́a²,

et al. 2008

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Since blood flow is tightly coupled to the health status of biological tissue, several instruments have been developed to monitor blood flow and perfusion dynamics. One such instrument is laser speckle imaging. The goal of this study was to evaluate the use of two velocity distribution assumptions (Lorentzian-and Gaussian-based) to calculate speckle flow index (SFI) values. When the normalized autocorrelation function for the Lorentzian and Gaussian velocity distributions satisfy the same definition of correlation time, then the same velocity range is predicted for low speckle contrast (0 < C < 0.6) and predict different flow velocity range for high contrast. Our derived equations form the basis for simplified calculations of SFI values.

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Modified laser speckle imaging method with improved spatial resolution

Cited by 231 publications

References 12 publications

Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy

Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy

Contrast Enhancement of Laser Speckle Contrast Image in Deep Vasculature by Reduction of Tissue Scattering

Impact of velocity distribution assumption on simplified laser speckle imaging equation

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