Modifications of the aniline acetate-furfural method for the determination of pentose

Borrow, A.; Jefferys, E. G.

doi:10.1039/an9568100598

Cited by 5 publications

(5 citation statements)

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“…3 mg/g WS was reached and dry weight accumulatio~l ceased for a period. All nutrients continued to be taken up during 7,1961 this period. After the dry weight 'step' the uptalte of all nutrients was linearly proportional to the dry weight until the time of glucose exhaustion.…”

Section: Cl~a?zges Ilz Ptimentioning

confidence: 91%

“…VOL. 7,1961 suggested that there might also be internal reserves of magnesium. The third experiment was designed t o explore these possibilities.…”

Section: Methodsmentioning

confidence: 97%

“…After the exhaustion of the linlitiilg nutrient in N-and M-limited fermentations, the phospholipid content of the fat decreased, until fillally varied, and sometimes large, increases dry weights greater than 20 mg/g WS. 7,1961 in fat (from 3.4-10 mg/g WS) were accolnpanied b y only s~n a l l increases in pllospl~olipid (from 33-39 pg P/g WS).…”

Section: Phospholipidmentioning

confidence: 97%

“…VOL. 7,1961 The maintenance phase is regarded a s a period in which external or internal carbon sources were metabolized by virtually unchanging living matter.…”

mentioning

confidence: 99%

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The Metabolism of Gibberella Fujikuroi in Stirred Culture

Borrow¹,

Jefferys²,

Kessell³

et al. 1961

Can. J. Microbiol.

Self Cite

111

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The growth of Gibberella fujikzlroi in stirred culture was studied. Media were designed in which the initial concentrations of glucose (G), nitrogen (N), phosphorus (P), and magnesium (M) were varied so that the first nutrient was exhausted a t a selected dry weight, and the remaining nutrients in all available sequences thereafter. Distinct phases of growth, related to the changing nutritional status of the mold, have been defined.The balanced phase was a period of proliferation in the presence of all nutrients, during which a unit increase in dry mycelium was accompanied by constant uptakes of G, N, P, M, and potassium (I<). The morphology remained virtually unchanged, and the d r y mycelium contained ca. 6 % fat, ca. 16% carbohydrate, and ca. 13% phosphorus-containing compounds, and the fractions of each remained constant. This phase continued until the first nutrient was exhausted. I'roliferation, indicated by increases in fat-and carbohydrate-free dry weight, then ceased in N-and G-limited fermentations. I n P-and M-limited fermentations, proliferation continued until the subsequent exhaustion of either G o r N, and during this transition phase the carbohydrate content of the mycelium increased, while in M-limited fermentations the fat content also increased. I n P-limited fermentations the uptake of both M and I< ceased, and reserves of metaphosphate were used.At the exhaustion of N in N-, P-, or M-limited fermentations, proliferation ceased but the dry weight continued to increase due t o increases in carbohydrate and triglyceride. In the presence of sufficient glucose, maximum amounts of 45% fat, visible as oil globules, and 32y0 carbohydrate were formed, but in the presence of less G these ceased t o increase when G was exhausted before these maxima were reached. In either case the nzaintenance phase followed, during which all mycelial components remained constant except the triglycerides, which decreased when exhaustion of G had initiated the maintenance phase; otherwise they also remained constant until G was subsequently exhausted, and then decreased. T h e terntinal phase began when the triglycerides were exhausted, and also when G was exhausted in G-limited fermentations, or in P-and M-limited fermentations in which G was exhausted before N. This phase was characterized by hyphal breakdown, a decrease in dry weight, and the liberation of mycelial components into the medium. 'Manuscript Can. J. Microbiol. Downloaded from www.nrcresearchpress.com by NC STATE UNIVERSITY on 09/21/13For personal use only. 80-1. FermenterThis was of conventional design, made of stainless steel, 20 in. diameter and 29 in. deep (Fig. 4). T h e two-paddle stirrer was driven a t 425 r.p.m. T h e supply of cooling water was automatically regulated to provide temperature control t o &0.15O C. Air was humidified and sterilized by passage through larger models of the apparatus already described. 2Model 0307, series WA, product of Hoover Ltd., Regent Street, London, W.1. Can. J. Microbiol. Downloaded from www.nrcresearchpress.com ...

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Section: Cl~a?zges Ilz Ptimentioning

confidence: 91%

“…VOL. 7,1961 suggested that there might also be internal reserves of magnesium. The third experiment was designed t o explore these possibilities.…”

Section: Methodsmentioning

confidence: 97%

Section: Phospholipidmentioning

confidence: 97%

“…VOL. 7,1961 The maintenance phase is regarded a s a period in which external or internal carbon sources were metabolized by virtually unchanging living matter.…”

mentioning

confidence: 99%

See 2 more Smart Citations

The Metabolism of Gibberella Fujikuroi in Stirred Culture

Borrow¹,

Jefferys²,

Kessell³

et al. 1961

Can. J. Microbiol.

Self Cite

111

View full text Add to dashboard Cite

show abstract

“…and Jeffreys [98], que também examinaram o efeito da concentração de anilina na reação com o furfural. Descobriu-se que aumentar a concentração de anilina resulta em maior intensidade de cor e um tempo mais curto para atingir o máximo de absorbância.…”

Section: Otimizaçãounclassified