Modifications of extracellular matrix features in the left and right uterine horns during the embryo pre-implantation period in Vicugna pacos

Barraza, Daniela Estefanía; Zampini, Renato; Apichela, Silvana Andrea; Pacheco, Joel I.; Argañaraz, Martin Eduardo

doi:10.1016/j.theriogenology.2020.07.001

Cited by 9 publications

(5 citation statements)

References 46 publications

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“…It was reported that the matrix metalloproteinase-2 (MMP-2) and MMP-9 levels in cell culture medium decreased signi cantly after overexpressing miR-155 [15,16], indicating that high expression of miR-155 could inhibit the activities of MMP-2 and MMP-9. MMP-2 and MMP-9 participated in the decidualization process of embryo implantation possibly by degrading extracellular matrix [17]. Our results showed that the expression levels of miR-155 decreased gradually with the process of decidualization, which was conducive to embryo implantation.…”

Section: Discussionmentioning

confidence: 60%

Expression and significance of miR-155 during embryo implantation

Lu¹

2022

Preprint

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Background miRNAs are considered to be one of the important molecules involved in the regulation of multiple physiological functions of endometrium such as proliferation and decidualization, angiogenesis, immune regulation, adhesion and invasion. miR-155 is closely related to the occurrence of some diseases such as inflammation, tumor metastasis and immunity. In view of the extreme similarity in immune escape, angiogenesis, cell proliferation, differentiation and invasion between embryo implantation and tumorigenesis, we studied the role of miR-155 in embryo implantation. Methods Human endometrial tissues and decidual tissues were collected, and the mouse models of early pregnancy were constructed. The expression levels of miR-155 were analyzed by quantitative PCR. Results The expression levels of miR-155 in decidual tissues were significantly lower than that of endometrial tissues in the middle secretory stage (P < 0.05). The results of mouse models showed that the endometrial decidualization progressed gradually with the increase of gestational days, and that the expression levels of miR-155 in endometrial tissues decreased gradually with the increase of gestational days (P < 0.05). On day 5 of pregnancy, the expression levels of miR-155 in endometrial tissues of embryo implantation sites were significantly lower than that in inter-implantation sites (P < 0.05). The amount of embryo implantation in the uterus injected by mmu-miR-155 agomir was significantly lower than that by normal control agomir (P < 0.05). Conclusions During the embryo implantation of early pregnancy, the expression level of miR-155 in endometrial tissues decreased significantly with the increase of pregnancy time, which may be related to endometrial decidualization.

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Section: Discussionmentioning

confidence: 60%

Expression and significance of miR-155 during embryo implantation

Lu¹

2022

Preprint

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“…MMPs are important in the uterine modifications that support angiogenesis and placentation. In alpacas MMPs are differentially expressed in the left and right uterine horns [ 51 ], which is interesting because while the chorion interdigitates with the epithelium in both horns, the embryo is carried on the left approximately 90% of the time.…”

Section: Discussionmentioning

confidence: 99%

Intra-horn insemination in the alpaca Vicugna pacos: Copulatory wounding and deep sperm deposition

Brennan,

Purdy,

Bacon

2024

PLoS ONE

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Alpacas (Vicugna pacos) are reported to be the rare mammal in which the penis enters the uterus in mating. To date, however, only circumstantial evidence supports this assertion. Using female alpacas culled for meat, we determined that the alpaca penis penetrates to the very tips of the uterine horns, abrading the tract and breaking fine blood vessels. All female alpacas sacrificed one hour or 24 hours after mating showed conspicuous bleeding in the epithelium of some region of their reproductive tract, including the hymen, cervix and the tips of each uterine horn, but typically not in the vagina. Unmated females showed no evidence of conspicuous bleeding. Histological examination of mated females revealed widespread abrasion of the cervical and endometrial epithelium, injuries absent in unmated females. Within one hour of mating, sperm were already present in the oviduct. The male alpaca’s cartilaginous penis tip with a hardened urethral process is likely responsible for the copulatory abrasion. The entire female reproductive tract interacts with the penis, functioning like a vagina. Alpacas are induced ovulators, and wounding may hasten delivery of the seminal ovulation-inducing factor beta-NGF into the female’s blood stream. There is no evidence of sexual conflict in copulation in alpaca, and thus wounding may also be one of a variety of mechanisms devised by mammals to induce a beneficial, short-term inflammatory response that stimulates blastocyst implantation, the uterine remodeling associated with placental development, and thus the success of early pregnancy.

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“…Epithelial-mesenchymal transition (EMT) and ECM remodeling are required for the initiation of cancer metastasis ( 39 ). Collagen is a major component of the ECM that is also involved in the development of human placenta ( 40 ). Matrix metalloproteinases (MMPs) are a family of calcium and zinc dependent proteases that play a key role in degrading the ECM proteins ( 41 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of key genes in HER2-positive breast cancer with brain metastasis via bioinformatics methods

Yang¹,

Sun²,

Qu³

et al. 2023

Transl Cancer Res

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Background Brain metastasis (BM) represents one of the most common advanced disease states in breast cancer (BC), especially in human epidermal growth factor receptor 2 (HER2)-positive BC, and is associated with poor survival outcomes. Methods In this study, in-depth analysis of the microarray data from the GSE43837 dataset with 19 BM samples of HER2-positive BC patients and 19 HER2-positive nonmetastatic primary BC samples was conducted. The differentially expressed genes (DEGs) between BM and primary BC samples were identified and function enrichment analysis of the DEGs was conducted to identify potential biological functions. The hub genes were identified by constructing the protein-protein interaction (PPI) network using STRING and Cytoscape. UALCAN and Kaplan-Meier plotter online tools were used to verify the clinical roles of the hub DEGs in HER2-positive BC with BM (BCBM). Results A total of 1,056 DEGs including 767 downregulated and 289 upregulated genes were identified by comparing the microarray data of the HER2-positive BM and primary BC samples. Functional enrichment analysis demonstrated that the DEGs were mainly enriched in pathways related to extracellular matrix (ECM) organization, cell adhesion, and collagen fibril organization. PPI network analysis identified 14 hub genes. Among these, CD44, COL1A2, MMP14, POSTN, and SOX9 were associated with the survival outcomes of HER2-positive patients. Conclusions In summary, 5 BM-specific hub genes were identified in the study; those are potential prognostic biomarkers and therapeutic targets for HER2-positive BCBM patients. However, further investigations are necessary to unravel the mechanisms by which these 5 hub genes regulate BM in HER2-positive BC.

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Modifications of extracellular matrix features in the left and right uterine horns during the embryo pre-implantation period in Vicugna pacos

Cited by 9 publications

References 46 publications

Expression and significance of miR-155 during embryo implantation

Expression and significance of miR-155 during embryo implantation

Intra-horn insemination in the alpaca Vicugna pacos: Copulatory wounding and deep sperm deposition

Identification of key genes in HER2-positive breast cancer with brain metastasis via bioinformatics methods

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