Modification of the membrane‐bound glucose oxidation system in <i>Gluconobacter oxydans </i>significantly increases gluconate and 5‐keto‐<scp>D</scp>‐gluconic acid accumulation

Merfort, Marcel; Herrmann, U.; Ha, Seung-Wook; Elfari, Mustafa; Bringer-Meyer, Stephanie; Görisch, Helmut; Sahm, Hermann

doi:10.1002/biot.200600032

Cited by 33 publications

(17 citation statements)

References 29 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5c). Similar effects had been observed previously in Gluconobacter oxydans MF1 when the gene coding for glucose dehydrogenase or gluconate-5-dehydrogenase was overexpressed (Merfort et al 2006).…”

Section: Enzyme Activitysupporting

confidence: 86%

Exotic glycerol dehydrogenase expressing Escherichia coli increases yield of 2,3-butanediol

Rahman

Qin

2018

Bioresour. Bioprocess.

View full text Add to dashboard Cite

Background:The thriving of biodiesel industry has led to produce 10% (v/v) crude glycerol, thus creating an overflow problem. Biofuel production is restricted by Escherichia coli due to its toxicity to bacterial cells. Therefore, a platform chemical and fuel additive 2,3-butanediol (2,3-BD) with low toxicity to microbes could be a promising alternative for biofuel production by recombinant E. coli using glycerol as the sole substrate.Results: A novel expression system of E. coli was developed to express the dhaD gene encoding glycerol dehydrogenase (GDH) to produce value-added metabolic products through aerobic biotransformation of glycerol. The dhaD gene obtained from Klebsiella pneumoniae SRP2 was expressed in E. coli BL21(DE3)pLysS using an E. coli-K. pneumoniae shuttle vector pJET1.2/blunt consisting of chloramphenicol-resistance gene under the control of the T7lac promotor. RT-PCR analysis and dhaD overexpression confirmed that the 2,3-BD synthesis pathway gene was expressed on RNA and protein levels. Therefore, the recombinant E. coli exhibited a 38.9-fold higher enzyme activity (312.57 units/ mg protein), yielding 8.97 g/L 2,3-BD, a 2.4-fold increase with respect to the non-recombinant strain. Conclusions:The engineered strain E. coli BL21(DE3)pLysS/pJET1.2/blunt-dhaD, carrying the 2,3-BD pathway gene dhaD from our newly isolated Klebsiella pneumoniae SRP2 strain, displayed the best ability to synthesize 2,3-BD from low-cost biomass glycerol. The value of expression of an important glycerol metabolism gene dhaD is the highest ever achieved with an engineered E. coli strain. From these results, the first reported dhaD expression system has paved the way for improvement of 2,3-BD production and is efficient for another heterologous gene expression in E. coli.

show abstract

Section: Enzyme Activitysupporting

confidence: 86%

Exotic glycerol dehydrogenase expressing Escherichia coli increases yield of 2,3-butanediol

Rahman

Qin

2018

Bioresour. Bioprocess.

View full text Add to dashboard Cite

show abstract

“…For industrial production of 5KGA, many attempts at improvement of 5KGA production have been done using genetically modified strains. [8][9][10][11] In our previous study, 92% conversion from D-glucose and D-gluconate to 5KGA was achieved in G-GA medium, 7) and in this study 5KGA was produced at an 87% conversion rate from D-glucose as sole substance using a wild-type Gluconobacter strain. Thus Gluconobacter without gene modification can be useful for high 5KGA conversion from D-glucose by a culture with controlled medium pH.…”

mentioning

confidence: 58%

Selective, High Conversion of D-Glucose to 5-Keto-D-gluoconate by Gluconobacter suboxydans

Ano

Shinagawa

Adachi

et al. 2011

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

Selective, high-yield production of 5-keto-D-gluconate (5KGA) from D-glucose by Gluconobacter was achieved without genetic modification. 5KGA production by Gluconobacter suffers byproduct formation of 2-keto-D-gluconate (2KGA). By controlling the medium pH strictly in a range of pH 3.5-4.0, 5KGA was accumulated with 87% conversion yield from D-glucose. The pH dependency of 5KGA formation appeared to be related to that of gluconate oxidizing activity.

show abstract

“…In this study however we found a previously described strain of G. oxydans (B58) to be more effective at leaching REE than the isolates. Further evaluation of G. oxydans for REE recovery applications is warranted; in addition to the robust leaching performance observed in this study, G. oxydans is also an attractive candidate for metabolic modeling and genetic engineering due to the availability of an annotated genome sequence (Prust et al, 2005) and developed genetic systems (Herrmann et al, 2004;Merfort et al, 2006;Prust et al, 2005;Schleyer et al, 2008;Zhang et al, 2010), as well as a recent developed metabolic network model (Wu et al, 2014). Genetic modifications and or manipulation of cultivation conditions to increase acid production and achieve even higher REE recovery will be considered.…”

Section: Discussionmentioning

confidence: 97%

Bioleaching of rare earth elements from waste phosphors and cracking catalysts

et al. 2016

View full text Add to dashboard Cite

Microbial cultures were evaluated for organic acid production and their potential utility for leaching of rare earth elements (REE) from retorted phosphor powder (RPP) and spent fluid catalytic cracking (FCC) catalyst. Two bacterial and one fungal strain were isolated from environmental and industrial materials known to contain REE and compared to the industrially important bacterium Gluconobacter oxydans. Gluconic acid was the predominant organic acid product identified in all of the cultures. Maximum REE leaching (49% of the total REE) from the FCC material was observed using cell-free culture supernatants of G. oxydans, with preferential recovery of lanthanum over cerium. The phosphor powder was more difficult to leach; only about 2% of the total REE was leached with G. oxydans. Leaching experiments with the RPP material indicated that the extent of REE solubilization was similar whether whole cell cultures or cell-free supernatants were used. Abiotic control experiments showed that increasing gluconic acid concentrations increased leaching efficiency; for example, total REE leaching from FCC catalyst increased from 24% to 45% when gluconic acid was increased from 10 mM to 90 mM. However, G. oxydans cell-free culture supernatants containing 10-15 mM gluconic acid were more effective than abiotically prepared leaching solutions with higher gluconic acid concentrations, suggesting that other exudate components were important too. Our results indicate that microorganisms producing gluconic and other organic acids can induce effective leaching of REE from waste materials, and that increasing organic acid production will improve recovery.

show abstract

Modification of the membrane‐bound glucose oxidation system in Gluconobacter oxydans significantly increases gluconate and 5‐keto‐D‐gluconic acid accumulation

Cited by 33 publications

References 29 publications

Exotic glycerol dehydrogenase expressing Escherichia coli increases yield of 2,3-butanediol

Exotic glycerol dehydrogenase expressing Escherichia coli increases yield of 2,3-butanediol

Selective, High Conversion of D-Glucose to 5-Keto-D-gluoconate by Gluconobacter suboxydans

Bioleaching of rare earth elements from waste phosphors and cracking catalysts

Contact Info

Product

Resources

About