Modification of sample processing for the<i>Limulus</i>amebocyte lysate assay enhances detection of inflammogenic endotoxin in intact bacteria and organic dust

Parr, Kimberly A Hoppe; Hađina, Suzana; Kilburg-Basnyat, Brita; Wang, Yifang; Chavez, Dulce; Thorne, Peter S.; Weiss, Jerrold

doi:10.1177/1753425917694084

Cited by 16 publications

(10 citation statements)

References 46 publications

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“…However, they extracted the dust samples in phosphate‐buffered saline and then diluted in water without adding a surfactant. Extraction of house dust into water without including a surfactant has been shown to markedly reduce endotoxin extraction efficiency …”

Section: Discussionsupporting

confidence: 88%

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Household endotoxin reduction in the Louisa Environmental Intervention Project for rural childhood asthma

et al. 2019

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Endotoxin exacerbates asthma. We designed the Louisa Environmental Intervention Project (LEIP) and assessed its effectiveness in reducing household endotoxin and improving asthma symptoms in rural Iowa children. Asthmatic school children (N = 104 from 89 homes) of Louisa and Keokuk counties in Iowa (aged 5‐14 years) were recruited and block‐randomized to receive extensive (education + professional cleaning) or educational interventions. Environmental sampling collection and respiratory survey administration were done at baseline and during three follow‐up visits. Mixed‐model analyses were used to assess the effect of the intervention on endotoxin levels and asthma symptoms in the main analysis and of endotoxin reduction on asthma symptoms in exploratory analysis. In the extensive intervention group, dust endotoxin load was significantly reduced in post‐intervention visits. The extensive compared with the educational intervention was associated with significantly decreased dust endotoxin load in farm homes and less frequent nighttime asthma symptoms. In exploratory analysis, dust endotoxin load reduction from baseline was associated with lower total asthma symptoms score (Odds ratio: 0.52, 95% confidence interval: 0.29‐0.92). In conclusion, the LEIP intervention reduced household dust endotoxin and improved asthma symptoms. However, endotoxin reductions were not sustained post‐intervention by residents.

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Section: Discussionsupporting

confidence: 88%

“…Wind speeds during sampling ranged from 0 to 5.4 m/s . Comparisons between the endotoxin values reported in the present study with those from other investigations must be interpreted cautiously due to very impactful laboratory differences in sample collection, storage, extraction, and assay techniques across studies …”

Section: Discussionmentioning

confidence: 47%

Household endotoxin reduction in the Louisa Environmental Intervention Project for rural childhood asthma

et al. 2019

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“…The value of measuring organic dust content in occupational settings was emphasized to make differences in health effects for exposed workers 47. Another recent study48 proposed the extraction method using Tris/EDTA to more accurately assess the endotoxin content and pro-inflammatory potential.…”

Section: Discussionmentioning

confidence: 99%

Association Between Organic Dust Exposure and Adult-Asthma: A Systematic Review and Meta-Analysis of Case-Control Studies

Zhang

Yan

Zheng

et al. 2019

Allergy Asthma Immunol Res

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BackgroundExposure to organic dust has been widely investigated as a potential risk factor for asthma with different results. To clarify a potential relationship, we performed the present meta-analysis to integrate the results of studies examining the association of organic dust exposure with asthma.MethodsA comprehensive literature search in the electronic databases including EMBASE, PubMed and Cochrane Library databases (up to August 2018) was conducted. The adjusted odds ratios (ORs) with corresponding 95% confidence interval (CI) for organic dust exposure and asthma were retrieved and pooled to generate summary effect estimates in Revman 5.2.ResultsDatabase searches retrieved 1,016 records. A total of 17 studies containing 3,619 cases and 6,585 controls were finally included in our meta-analysis. The summary estimates suggested that organic dust exposure was positively associated with asthma (OR, 1.48; 95% CI, 1.26–1.75; P < 0.00001), whether among population-based case-control studies (OR, 1.24; 95% CI, 1.13–1.35; P < 0.00001) or hospital-based case-control studies (OR, 2.79; 95% CI, 1.27–6.12; P = 0.01). Subgroup analysis showed that paper/wood (OR, 1.62; 95% CI, 1.38–1.90; P < 0.00001), flour/grain (OR, 1.48; 95% CI, 1.11–1.97; P = 0.008), and textile dust (OR, 1.50; 95% CI, 1.08–2.09; P = 0.02) exposure were significantly associated with asthma.ConclusionsBased on the studies evaluated, our meta-analysis results prompt that organic dust exposure is a risk factor inducing asthma, although precise analysis focus on specific organic dust materials is still warranted.

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“…The dust and air samples from home and school will be shipped to the University of Iowa for analysis of endotoxin using a kinetic chromogenic Limulus amebocyte lysate assay,[26,27] (1 → 3)-β-glucan using the Limulus Factor G assay, and ten inhalant allergens using the MARIA multiplex bead-based assay (Indoor Biotechnologies, Inc.).…”

Section: Study Design and Methodsmentioning

confidence: 99%

The School Inner-City Asthma Intervention Study: Design, rationale, methods, and lessons learned

Phipatanakul

Koutrakis

Coull

et al. 2017

Contemporary Clinical Trials

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Asthma is the most common chronic disease of childhood in the United States, causes significant morbidity, particularly in the inner-city, and accounts for billions of dollars in health care utilization. Home environments are established sources of exposure that exacerbate symptoms and home-based interventions are effective. However, elementary school children spend 7 to 12 h a day in school, primarily in one classroom. From the observational School Inner-City Asthma Study we learned that student classroom-specific exposures are associated with worsening asthma symptoms and decline in lung function. We now embark on a randomized, blinded, sham-controlled school environmental intervention trial, built on our extensively established school/community partnerships, to determine the efficacy of a school-based intervention to improve asthma control. This factorial school/classroom based environmental intervention will plan to enroll 300 students with asthma from multiple classrooms in 40 northeastern inner-city elementary schools. Schools will be randomized to receive either integrated pest management versus control and classrooms within these schools to receive either air purifiers or sham control. The primary outcome is asthma symptoms during the school year. This study is an unprecedented opportunity to test whether a community of children can benefit from school or classroom environmental interventions. If effective, this will have great impact as an efficient, cost-effective intervention for inner city children with asthma and may have broad public policy implications.

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Modification of sample processing for theLimulusamebocyte lysate assay enhances detection of inflammogenic endotoxin in intact bacteria and organic dust

Cited by 16 publications

References 46 publications

Household endotoxin reduction in the Louisa Environmental Intervention Project for rural childhood asthma

Household endotoxin reduction in the Louisa Environmental Intervention Project for rural childhood asthma

Association Between Organic Dust Exposure and Adult-Asthma: A Systematic Review and Meta-Analysis of Case-Control Studies

The School Inner-City Asthma Intervention Study: Design, rationale, methods, and lessons learned

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