Moderate body hypothermia alleviates behavioral and EEG manifestations of audiogenic seizures in metaphit-treated rats

Abstract

We investigated the effects of hypothermia on the incidence and EEG signs of audiogenic seizures in rats treated with metaphit (1-[1(3isothiocyanatophenyl)-cyclohexyl] piperidine), an experimental model of generalized reflex epilepsy. After i.p. injection with metaphit (10 mg/kg) Wistar rats were exposed to audiogenic stimulation at hourly intervals during the time course of the experiment. After intermittent use of an ice pack 8 h after the metaphit treatment, when seizure was fully developed, the body temperature was reduced to 30 +/- 0.5 degrees C in one half of the rats, and maintained at 37 +/- 0.5 degrees C in the other half. Saline-injected rats served as a control group. In the hypothermia group, the incidence of audiogenic seizures induced by metaphit was completely suppressed during the 3 consecutive testing times, while no signs of epileptiform activity were noted in EEG tracings. The termination of hypothermic treatment resulted in the recovery of seizure susceptibility, and during audiogenic stimulation, bursts of spiking activity were recorded in the EEGs of metaphit-treated rats. These findings indicate that moderate body hypothermia is an effective anticonvulsant treatment for audiogenic seizures in metaphit-treated rats.

Neurogenesis and the impact of steroid hormones on behaviour

“…Neurosteroidi (alopregnanolon, androstenedione, alotetrahidrodeoksikortikosteron -THDOC) pozitivnom alosteronom modulacijoim GABA-A receptora smanjuju ćelijsku ekscitabilnost (107). U našoj labaroatoriji su razvijeni metafitom indukovan, homocisteinski i lindanski modeli epilepsije, te ispitani brojni antikonvulzivni i prokonvulzivni faktori (108)(109)(110)(111)(112)(113). Administracija alopregnanolola u homocisteinskom modelu epilepsije kod pacova nije dala očekivane antikonvulzivne efekte (neobjaveljeni rezultati).…”

Neurogenesis and the impact of steroid hormones on behaviour

“…Neurosteroidi (alopregnanolon, androstenedione, alotetrahidrodeoksikortikosteron -THDOC) pozitivnom alosteronom modulacijoim GABA-A receptora smanjuju ćelijsku ekscitabilnost (107). U našoj labaroatoriji su razvijeni metafitom indukovan, homocisteinski i lindanski modeli epilepsije, te ispitani brojni antikonvulzivni i prokonvulzivni faktori (108)(109)(110)(111)(112)(113). Administracija alopregnanolola u homocisteinskom modelu epilepsije kod pacova nije dala očekivane antikonvulzivne efekte (neobjaveljeni rezultati).…”

Modulation of Epileptic Activity in Rats: Focus on Sleep, Physical Exercise and Nitric Oxide–mediated Neurotransmission in a Model of Homocysteine Thiolactone–induced Seizures

“…Modelling the epileptic activity in animals involves two main approaches: administering different chemical compounds and electrical stimulation kindling. In our Laboratory for Neurophysiology, different animal models have been developed, including metaphit audiogenic seizures (18)(19)(20)(21) and lindane- (22)(23)(24) and homocysteine thiolactoneinduced seizures (11). In addition to these models, the other commonly used experimental epilepsy models are those induced by factors such as N-methyl-D-aspartate (NMDA), pentylenetetrazol, pilocarpine, kainic acid, and 4-aminopyridine amygdala kindling (for review see 4).…”

Neurogenesis and the impact of steroid hormones on behaviour

“…Neurosteroidi (alopregnanolon, androstenedione, alotetrahidrodeoksikortikosteron -THDOC) pozitivnom alosteronom modulacijoim GABA-A receptora smanjuju ćelijsku ekscitabilnost (107). U našoj labaroatoriji su razvijeni metafitom indukovan, homocisteinski i lindanski modeli epilepsije, te ispitani brojni antikonvulzivni i prokonvulzivni faktori (108)(109)(110)(111)(112)(113). Administracija alopregnanolola u homocisteinskom modelu epilepsije kod pacova nije dala očekivane antikonvulzivne efekte (neobjaveljeni rezultati).…”

Modulation of Epileptic Activity in Rats: Focus on Sleep, Physical Exercise and Nitric Oxide–mediated Neurotransmission in a Model of Homocysteine Thiolactone–induced Seizures

“…Modelling the epileptic activity in animals involves two main approaches: administering different chemical compounds and electrical stimulation kindling. In our Laboratory for Neurophysiology, different animal models have been developed, including metaphit audiogenic seizures (18)(19)(20)(21) and lindane- (22)(23)(24) and homocysteine thiolactoneinduced seizures (11). In addition to these models, the other commonly used experimental epilepsy models are those induced by factors such as N-methyl-D-aspartate (NMDA), pentylenetetrazol, pilocarpine, kainic acid, and 4-aminopyridine amygdala kindling (for review see 4).…”

Neurogenesis and the impact of steroid hormones on behaviour

“…Neurosteroidi (alopregnanolon, androstenedione, alotetrahidrodeoksikortikosteron -THDOC) pozitivnom alosteronom modulacijoim GABA-A receptora smanjuju ćelijsku ekscitabilnost (107). U našoj labaroatoriji su razvijeni metafitom indukovan, homocisteinski i lindanski modeli epilepsije, te ispitani brojni antikonvulzivni i prokonvulzivni faktori (108)(109)(110)(111)(112)(113). Administracija alopregnanolola u homocisteinskom modelu epilepsije kod pacova nije dala očekivane antikonvulzivne efekte (neobjaveljeni rezultati).…”

Modulation of Epileptic Activity in Rats: Focus on Sleep, Physical Exercise and Nitric Oxide–mediated Neurotransmission in a Model of Homocysteine Thiolactone–induced Seizures

“…Modelling the epileptic activity in animals involves two main approaches: administering different chemical compounds and electrical stimulation kindling. In our Laboratory for Neurophysiology, different animal models have been developed, including metaphit audiogenic seizures (18)(19)(20)(21) and lindane- (22)(23)(24) and homocysteine thiolactoneinduced seizures (11). In addition to these models, the other commonly used experimental epilepsy models are those induced by factors such as N-methyl-D-aspartate (NMDA), pentylenetetrazol, pilocarpine, kainic acid, and 4-aminopyridine amygdala kindling (for review see 4).…”