Models of Interaction between Higher Plants and Bacteria

Rudolph, K.

doi:10.1007/978-1-4684-2769-1_8

Cited by 6 publications

(7 citation statements)

References 42 publications

(12 reference statements)

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“…Mannitol was used as a carbon source in MM medium to promote polysaccharide production in the parent strain ANU280, thus enhancing the contrast between the parental and mutant strain colony morphologies. Since a role for EPS and associated capsular material has been inferred in several pathogenic bacterialplant interactions (1,2,7,18,29,36), we investigated the possibility that strain ANU2861 produces an altered EPS structure compared with that produced by the parent strain ANU280. A thorough structural analysis of the EPSs and the oligosaccharide repeat units produced by both parent strain ANU280 and mutant strain ANU2861 showed that these molecules were identical to those previously published for ANU280 (12,13) under the conditions used (data not shown).…”

Section: Resultsmentioning

confidence: 99%

Induction of pathogenic-like responses in the legume Macroptilium atropurpureum by a transposon-induced mutant of the fast-growing, broad-host-range Rhizobium strain NGR234

et al. 1988

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Mutant strain ANU2861, a transposon Tn5 mutant of the fast-growing, broad-host-range Rhizobium strain ANU280 (NGR234 Smr Rfr) overproduces polysaccharide, is an ade auxotroph, and induces poorly developed nodules on Leucaena leucocephala and Lablab purpureus (H.C. Chen, M. Batley, J.W. Redmond, and B.G. Rolfe, J. Plant Physiol. 120:331-349, 1985). Strain ANU2861 cannot form nodules on Macroptilium atropurpureum Urb. (siratro) or on Desmodium intortum and D. uncinatum and the nonlegume Parasponia. The parent strain, ANU280, effectively nodulates all these legume species except Parasponia, on which it forms ineffective nodules. Ultrastructural examination of infection sites on the legume siratro showed that mutant strain ANU2861 caused root hair curling (Hac+ phenotype), some cortical cell division (Noi+), but no infection threads (Inf-). Localized cellular responses, known to occur in phytopathological interactions, were observed in electron micrographs of the epidermal tissue at or near the infection zone after inoculation with strain ANU2861 but not the wild-type parental strain. These include (i) the rapid (within 20 h) accumulation of osmiophilic droplets attached to membranes at potential sites of strain ANU2861 penetration and (after 48 h) in the epidermal cells in the immediate region of the curled root hairs, and (ii) localized cell death of the epidermal cells. In addition, strain ANU2861 can initiate a systemic response in split-root siratro plants which prevents the successful nodulation of strain ANU280. A 6.3-kilobase fragment of wild-type genomic DNA, which includes the site of Tn5 insertion in strain ANU2861, was cloned and introduced to strain ANU2861. All the phenotypic defects of the mutant strain were corrected by the introduction of this DNA fragment. This indicates that the original Tn5 insertion is responsible for the phenotype.

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Section: Resultsmentioning

confidence: 99%

Induction of pathogenic-like responses in the legume Macroptilium atropurpureum by a transposon-induced mutant of the fast-growing, broad-host-range Rhizobium strain NGR234

et al. 1988

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“…Rudolph [21] has argued that the desiccation of tissues undergoing the HR in response to high concentrations of bacteria creates a physical environment unsuited for bacterial multiplication. Where low concentrations of bacteria are used and isolated cells respond hypersensitively within largely intact tissue [61,92,95,96] desiccation is unlikely to be a major cause of restricted growth.…”

Section: Ultrastructural Studiesmentioning

confidence: 99%

“…Plant cells within the water soaked lesion become depleted of starch and undergo changes in chloroplast and cytoplasmic structure similar to those occurring during senescence but there are no signs of rapid cytoplasmic disorganization. EPS, produced in abundance by the invading bacteria, probably act as a hydrophilic matrix (sponge) maintaining a supply of water essential for bacterial growth [2,21). Production of EPS in vivo may therefore be considered an essential requirement for pathogenicity.…”

Section: Mechanisms Of Pathogenicitymentioning

confidence: 99%

The Biology of Interactions between Plants and Bacteria

Mansfıeld

Brown

1986

Biology and Molecular Biology of Plant-Pathogen Interactions

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“…The yellow halo is produced by a nonspecific peptide-type toxin (MITCHELL 1976, RUDOLPH 1976b, RASCHE 1977, but medianism responsible for induction of the water-soaked lesion is unknown. Induction of water-soaking in the intercellular spaces of host tissue must be regarded as a "sine qua non" for the bacterial multiplication to high concentrations, whidi occur in compatible combinations (JOHNSON 1937, RUDOLPH 1976a.…”

mentioning

confidence: 99%

A Host Specific Principle from Pseudomonas phaseolicola (Burkh.) Dowson, Inducing Water-Soaking in Bean Leaves

Rudolph

1978

J Phytopathol

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Models of Interaction between Higher Plants and Bacteria

Cited by 6 publications

References 42 publications

Induction of pathogenic-like responses in the legume Macroptilium atropurpureum by a transposon-induced mutant of the fast-growing, broad-host-range Rhizobium strain NGR234

Induction of pathogenic-like responses in the legume Macroptilium atropurpureum by a transposon-induced mutant of the fast-growing, broad-host-range Rhizobium strain NGR234

The Biology of Interactions between Plants and Bacteria

A Host Specific Principle from Pseudomonas phaseolicola (Burkh.) Dowson, Inducing Water-Soaking in Bean Leaves

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