Models of Endometriosis: Animal Models II – Non‐Human Primates

Fazleabas, Asgerally T.

doi:10.1002/9781444398519.ch27

Cited by 3 publications

(1 citation statement)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, the use of immuno-deficient mice confounds the investigation of immuno-regulatory component involved in the pathogenesis of the disease. 16 Therefore, there is a need for a suitable animal model that develops macroscopically visible lesions characteristic of endometriosis in women which will enable repeated and sequential imaging for diagnostic and treatment trials.…”

Section: Introductionmentioning

confidence: 99%

Development of a domestic animal model for endometriosis: Surgical induction in the dog, pigs, and sheep

Varughese

Adams

Leonardi

et al. 2018

Journal of Endometriosis and Pelvic Pain Disorders

View full text Add to dashboard Cite

Background: Endometriosis affects one in ten women of reproductive age but it is diagnosed at advanced stages. Our objective was to develop a domestic animal model that would permit sequential assessment of endometriotic changes. Materials and methods: Surgical transplantation of endometrial tissue and fat (n = 4 grafts/tissue/animal) was done in dogs (n = 5), pigs (n = 4), and sheep (n = 5). Autologous grafts were sutured to the visceral (urinary bladder in dogs and pigs and uterus in sheep) and parietal peritoneum. Sham surgeries were performed (dogs and sheep n = 5 and pigs n = 3) by placing fat grafts alone. Plasma estrogen and progesterone concentration was performed prior to surgery and weekly following surgery until euthanasia. Animals were euthanized between 80 and 110 days after surgery. Gross and histopathologic features of endometriotic lesions were recorded. Results: A variety of lesions from transplanted endometrial grafts included endometriotic cysts, vesicles, solid lesions, or absence of lesions. The proportion of cysts was greater (p < 0.01) in dogs (18/20 grafts) than in pigs (5/16) and sheep (5/20). The area of endometriotic lesions at the time of euthanasia was greater than at the time of surgery in dogs (0.89 ± 0.11 cm 2 vs 0.50 ± 0.09 cm 2 ; p < 0.05), whereas, the size of lesions decreased (p < 0.05) by half or more in pigs and sheep. In dogs, endometrial cysts were characterized by simple cuboidal/columnar epithelium, endometrial glands, stromal tissue with hemorrhage and/or hemosiderin-laden macrophages, and smooth muscle cells. Conclusion: The development of endometriotic cysts was apparent in dogs than in sheep and pigs. Therefore, dog is a better domestic animal model for endometriosis.

show abstract

Section: Introductionmentioning

confidence: 99%

Development of a domestic animal model for endometriosis: Surgical induction in the dog, pigs, and sheep

Varughese

Adams

Leonardi

et al. 2018

Journal of Endometriosis and Pelvic Pain Disorders

View full text Add to dashboard Cite

show abstract

Endometriosis

Zondervan

Becker

Koga

et al. 2018

Nat Rev Dis Primers

853

746

View full text Add to dashboard Cite

Endometriosis is a common inflammatory disease characterized by the presence of tissue outside the uterus that resembles endometrium, mainly on pelvic organs and tissues. It affects ~5-10% of women in their reproductive years - translating to 176 million women worldwide - and is associated with pelvic pain and infertility. Diagnosis is reliably established only through surgical visualization with histological verification, although ovarian endometrioma and deep nodular forms of disease can be detected through ultrasonography and MRI. Retrograde menstruation is regarded as an important origin of the endometrial deposits, but other factors are involved, including a favourable endocrine and metabolic environment, epithelial-mesenchymal transition and altered immunity and inflammatory responses in genetically susceptible women. Current treatments are dictated by the primary indication (infertility or pelvic pain) and are limited to surgery and hormonal treatments and analgesics with many adverse effects that rarely provide long-term relief. Endometriosis substantially affects the quality of life of women and their families and imposes costs on society similar to those of other chronic conditions such as type 2 diabetes mellitus, Crohn's disease and rheumatoid arthritis. Future research must focus on understanding the pathogenesis, identifying disease subtypes, developing non-invasive diagnostic methods and targeting non-hormonal treatments that are acceptable to women who wish to conceive.

show abstract

Unveiling the fibrotic puzzle of endometriosis: An overlooked concern calling for prompt action

Anchan,

Kalthur,

Datta

et al. 2024

F1000Res

View full text Add to dashboard Cite

Endometriosis is a benign, estrogen-dependent, persistent chronic inflammatory heterogeneous condition that features adhesions caused by estrogen-dependent periodic bleeding. It is characterised by a widely spread fibrotic interstitium that comprising of fibroblasts, myofibroblasts, collagen fibres, extracellular proteins, inflammatory cells, and active angiogenesis found outside the uterus. Thus, fibrosis is recognized as a critical component because of which current treatments, such as hormonal therapy and surgical excision of lesions are largely ineffective with severe side effects, high recurrence rates, and significant morbidity. The symptoms include dysmenorrhea (cyclic or non-cyclic), dyspareunia, abdominal discomfort, and infertility. The significant lack of knowledge regarding the underlying root cause, etiology, and complex pathogenesis of this debilitating condition, makes it challenging to diagnose early and to implement therapeutic approaches with minimal side effects presenting substantial hurdles in endometriosis management. Research on understanding the pathogenesis of endometriosis is still ongoing to find biomarkers and develop non-hormonal therapeutic approaches. Current clinical research indicates a close relationship between endometriosis and fibrosis, which is thought to be tightly linked to pain, a major factor for the decline in the patient’s quality of life but little is known about the underlying pathophysiological cellular and molecular signaling pathways that lead to endometriosis-related fibrosis. The available experimental disease models have tremendous challenges in reproducing the human characteristics of the disease to assess treatment effectiveness. Future translational research on the topic has been hindered by the lack of an adequate fibrotic model of endometriosis emphasizing the necessity of etiological exploration. This review article’s goal is to examine recent developments in the field and pinpoint knowledge gaps that exist with a focus on the development of novel fibrotic mouse models for the early diagnosis and treatment of endometriosis and how this knowledge aids in the development of novel anti-fibrotic treatments which opens fresh avenues for a thorough investigation and extended research in the field of endometriosis.

show abstract

Models of Endometriosis: Animal Models II – Non‐Human Primates

Cited by 3 publications

References 63 publications

Development of a domestic animal model for endometriosis: Surgical induction in the dog, pigs, and sheep

Development of a domestic animal model for endometriosis: Surgical induction in the dog, pigs, and sheep

Endometriosis

Unveiling the fibrotic puzzle of endometriosis: An overlooked concern calling for prompt action

Contact Info

Product

Resources

About