Modelling the interaction between bacterial cells and laser-textured surfaces

Lazzini, Gianmarco; Romoli, Luca; Lutey, Adrian H. A.; Fuso, Francesco

doi:10.1016/j.surfcoat.2019.06.078

Cited by 21 publications

(8 citation statements)

References 34 publications

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“…To go further than limiting bacterial adhesion, it is known that some bioinspired nanopillar structures can have a bactericidal effect. Density, spatial period and depth of ripples could be correlated with the "fakir effect" on the bacterium (like the bacterium is laying on a bed of nails) that disrupts membrane and thus proliferation [45]. For instance, nanopillars with a size of 60-215 nm and spaced out 100-380 nm, create a "mechanical bactericidal mechanism" that ruptures or…”

Section: Discussionmentioning

confidence: 99%

Dual-functionalized titanium by ultrafast laser texturing to enhance human gingival fibroblasts adhesion and minimize Porphyromonas gingivalis colonization

Papa

Khalil

Hamzeh‐Cognasse

et al. 2022

Applied Surface Science

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Section: Discussionmentioning

confidence: 99%

Dual-functionalized titanium by ultrafast laser texturing to enhance human gingival fibroblasts adhesion and minimize Porphyromonas gingivalis colonization

Papa

Khalil

Hamzeh‐Cognasse

et al. 2022

Applied Surface Science

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“…Laser surface texturing is a promising method to tailor wettability, surface energy, surface topography, and morphology through which cell attachment and proliferation can be modulated [ 21 , 22 ]. Moreover, ultrashort pulsed laser treatment is a good candidate for the production of man-made antibacterial surfaces as it can be used to produce textures whose sizes are lower than bacterial size [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Surface Properties of Ti6Al7Nb Alloy: Surface Free Energy and Bacteria Adhesion

Krzywicka

Szymańska

Tofil

et al. 2022

JFB

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The laser micro-machining was carried out on a station equipped with a TruMicro 5325c laser emitting ultraviolet radiation (343 nm wavelength) in picosecond pulses. On the surface of the Ti6Al7Nb alloy, dimple texturing with a constant diameter of ~200 μm, different depths (from ~5 to ~78 μm) and density (from 10% to 50%) were produced. The value of surface free energy was determined with the Owens–Wendt method using two measuring liquids: distilled water and diodomethane. The Staphylococcus epidermidis strain was used to test the adhesion of bacteria. It was found that the surface free energy value is influenced by both of the texture parameters (density, depth). The density also affects the potential for biofilm formation. Based on the analysis, it was shown that with an increase in surface free energy, the number of adhering microorganisms increases exponentially. Moreover, the study shows that there is a correlation between the number of adhering microorganisms and surface free energy.

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“…In its elaborate review, N. Sirdeshmukh and G. Dongre outline the biocompatibility and antibacterial analysis of laser surface textured patterns made by different research groups [ 75 ]. The scientists are pointing out that the right combination of micro and nano roughness could lead to the desired antibacterial effect, by means of LIPSS generation in combination with different nanostructures-nanopillars [ 76 ], spike nanotextured patterns [ 77 ], nanoripples [ 78 ], conic and spherical nanostructures [ 79 ], etc.—all mimicking the topography of natural structured surfaces exhibiting antibacterial proper-ties [ 80 ]. On the other hand, as already mentioned, the generation of laser-induced periodic structures, on the polymer surface such as PLA could be a difficult task to achieve, which could be attributed to their low melting (T m ) and glass transition (T g PLA = 60 °C) temperature [ 35 ].…”

Section: Resultsmentioning

confidence: 99%

Biomimetic Hierarchical Structuring of PLA by Ultra-Short Laser Pulses for Processing of Tissue Engineered Matrices: Study of Cellular and Antibacterial Behavior

et al. 2021

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The influence of ultra-short laser modification on the surface morphology and possible chemical alteration of poly-lactic acid (PLA) matrix in respect to the optimization of cellular and antibacterial behavior were investigated in this study. Scanning electron microscopy (SEM) morphological examination of the processed PLA surface showed the formation of diverse hierarchical surface microstructures, generated by irradiation with a range of laser fluences (F) and scanning velocities (V) values. By controlling the laser parameters, diverse surface roughness can be achieved, thus influencing cellular dynamics. This surface feedback can be applied to finely tune and control diverse biomaterial surface properties like wettability, reflectivity, and biomimetics. The triggering of thermal effects, leading to the ejection of material with subsequent solidification and formation of raised rims and 3D-like hollow structures along the processed zones, demonstrated a direct correlation to the wettability of the PLA. A transition from superhydrophobic (θ > 150°) to super hydrophilic (θ < 20°) surfaces can be achieved by the creation of grooves with V = 0.6 mm/s, F = 1.7 J/cm2. The achieved hierarchical architecture affected morphology and thickness of the processed samples which were linked to the nature of ultra-short laser-material interaction effects, namely the precipitation of temperature distribution during material processing can be strongly minimized with ultrashort pulses leading to non-thermal and spatially localized effects that can facilitate volume ablation without collateral thermal damage The obtained modification zones were analyzed employing Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), Energy dispersive X-ray analysis (EDX), and optical profilometer. The modification of the PLA surface resulted in an increased roughness value for treatment with lower velocities (V = 0.6 mm/s). Thus, the substrate gains a 3D-like architecture and forms a natural matrix by microprocessing with V = 0.6 mm/s, F = 1.7 J/cm2, and V = 3.8 mm/s, F = 0.8 J/cm2. The tests performed with Mesenchymal stem cells (MSCs) demonstrated that the ultra-short laser surface modification altered the cell orientation and promoted cell growth. The topographical design was tested also for the effectiveness of bacterial attachment concerning chosen parameters for the creation of an array with defined geometrical patterns.

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Modelling the interaction between bacterial cells and laser-textured surfaces

Cited by 21 publications

References 34 publications

Dual-functionalized titanium by ultrafast laser texturing to enhance human gingival fibroblasts adhesion and minimize Porphyromonas gingivalis colonization

Dual-functionalized titanium by ultrafast laser texturing to enhance human gingival fibroblasts adhesion and minimize Porphyromonas gingivalis colonization

Surface Properties of Ti6Al7Nb Alloy: Surface Free Energy and Bacteria Adhesion

Biomimetic Hierarchical Structuring of PLA by Ultra-Short Laser Pulses for Processing of Tissue Engineered Matrices: Study of Cellular and Antibacterial Behavior

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