Modeling of antiproliferative effects of Salvia officinalis L. essential oil optimized using Box–Behnken design

Abstract

BackgroundSalvia officinalis L. essential oil (SoEO) was mostly traditionally used to medicate various diseases as cancer. Then, the present work aims were: (1) to model the cytotoxicity effects of Salvia officinalis L. essential oil (SoEO) related to the human cancer cell lines kind (MCF-7 and HeLa) ; (2) to optimize the hydro-distillation extraction conditions of SoEO; and, (3) to determine the in vitro scavenging capacity of the free radicals DPPH • , NO • , ABTS + , and the ability to reduce Fe 3+ . MethodsThe cytotoxicity and anti-proliferative abilities were evaluated by measuring cell viability and then modeled. Two human cell lines: MCF-7 and HeLa were used. The optimization of SoEO extraction by hydro-distillation was carried out with Response Surface Methodology (RSM) using the Box-Behnken design ResultsThe cytotoxicity activity against both tumor cell lines MCF-7 and HeLa was considerably important with IC50 = 3.125 and 8.920 μg/mL, respectively. All treated cell lines showed a significant reducing in cell viability in response to the increasing oil concentration. The relative behaviors of both cell lines under SoEO treatment were modeled. The obtained optimal extraction yield was Y = 1.85 g/100 g d.b. The main identified fractions were camphene (23.7%), α-thujone (19.62%), 1,8-cineole (10.6 %), viridiflorol (5.9%), borneol (5.72%); β-thujone (5.4%); caryophyllene (3,83%). Also, SoEO was mostly able to scavenge DPPH • free radical, ABTS + radical and hydrogen peroxide in an amount dependent manner (IC50 = 0.97, 0.279 and 0.05 mg/mL, respectively).

Chemical Composition and Biological Activities of the Essential Oil from Citrus reticulata Blanco Peels Collected from Agrowastes

“…[9] By virtue of these bioactive compounds, the citrus fruits are known to have antioxidant, [10] antimicrobial, [11] antineoplastic, [12,13] anti-diabetic [14,15] and other pharmacological properties. [16][17][18] Apart from their fruits, the peel is also a rich source of various terpenoids and derivatives. The compound D-Limonene is the major component of the peel.…”

“…The prominent compounds in the citrus fruits are polyphenols and flavonoids [9] . By virtue of these bioactive compounds, the citrus fruits are known to have antioxidant, [10] antimicrobial, [11] antineoplastic, [12,13] anti‐diabetic [14,15] and other pharmacological properties [16–18] . Apart from their fruits, the peel is also a rich source of various terpenoids and derivatives.…”