Modeling microbial ethanol production by E. coli under aerobic/anaerobic conditions: Applicability to real postmortem cases and to postmortem blood derived microbial cultures

Boumba, Vassiliki A.; Kourkoumelis, Nikolaos; Gousia, Panagiota; Εconomou, Vangelis; Papadopoulou, Chrissanthy; Vougiouklakis, Theodore

doi:10.1016/j.forsciint.2013.07.021

Cited by 34 publications

(43 citation statements)

References 22 publications

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“…The production of ethanol and other volatiles from a variety of substrates (namely carbohydrates, amino acids, glycerol and fatty acids) represents a common phenomenon amongst bacteria (more than 58 species; e.g., Corynebacterium sp., Lactococcus garviae, Escherichia coli, Enterococcus faecalis, Klebsiella oxytoca, Klebsiella pneumoniae, Proteus mirabilis), yeasts (more than 17 species; e.g., Candida albicans, Candida tropicalis, Candida tropicana, Candida glabrata, Candida parapsilosis, Saccharomyces cerevisiae) and fungi (more than 29 species; e.g., Aspergillus awamori, Aspergillus foetidus, Aspergillus nidulans, Aspergillus oryzae, Aspergillus sojae, Aspergillus tamari, Fusarium sp., Rhizopus javanicus and Rhizopus oryzae) (Boumba et al 2012;Boumba et al 2008;Skory et al 1997). Therefore, the origin of postmortem ethanol can be attributed either to antemortem ingestion or postmortem microbial production (Boumba et al 2013).…”

Section: Toxicologymentioning

confidence: 99%

Microbial forensics: new breakthroughs and future prospects

Oliveira

Amorim

2018

Appl Microbiol Biotechnol

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Recent advances in genetic data generation, through massive parallel sequencing (MPS), storage and analysis have fostered significant progresses in microbial forensics (or forensic microbiology). Initial applications in circumstances of biocrime, bioterrorism and epidemiology are now accompanied by the prospect of using microorganisms (i) as ancillary evidence in criminal cases; (ii) to clarify causes of death (e.g., drownings, toxicology, hospital-acquired infections, sudden infant death and shaken baby syndromes); (iii) to assist human identification (skin, hair and body fluid microbiomes); (iv) for geolocation (soil microbiome); and (v) to estimate postmortem interval (thanatomicrobiome and epinecrotic microbial community). When compared with classical microbiological methods, MPS offers a diverse range of advantages and alternative possibilities. However, prior to its implementation in the forensic context, critical efforts concerning the elaboration of standards and guidelines consolidated by the creation of robust and comprehensive reference databases must be undertaken.

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Section: Toxicologymentioning

confidence: 99%

Microbial forensics: new breakthroughs and future prospects

Oliveira

Amorim

2018

Appl Microbiol Biotechnol

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“…Response surface analysis Multivariate design of experimentscoupled to response surface methodologyis a widely used method in optimization processes, once it consumes less time, requires a smaller set of experimental procedures and resources, allows the obtainment of large quantities of data in a single step process and enables the discoverof the most desirable conditions, or desirability (Yin et al, 2009;Mohamad et al, 2011;Boumba et al, 2013;Cheng et al, 2013).Hence, a response surface methodology (RSM) was used to determine the influence of nutrient sources (peptone, yeast extract and both extruded bean) in the E.coli growth. Despite the good results of optical density in the media containing common bean and yeast extract or peptone in equivalent proportions, the surface response confirms the highest growth profile using extruded common beanas sole nitrogen source (Figure 1a).The projection evidenced a negative effect of peptone (run 1) and yeast extract (run 2) as isolated sources, while common bean shows a trend to a further growth by increasing its concentration.In this scenario, the mathematical prediction of an optimum composition is presented as 0.25% yeast extract and 0.75% of extruded bean.…”

Section: 2mentioning

confidence: 99%

“…However, the microbial production of amylases can be affected by certain factors, including microbial strain, culture medium formula and physicochemical conditions (Hii et al, 2012). Considering the composition of culture medium, the nature of nitrogen and carbon source could influence the rate of amylase production and, therefore the enhancement of target enzyme productivity in any fermentation system could be achieved through the improvement of the culture medium composition (Ye et al, 2010;Hortsch and Weuster-Botz, 2011;Boumba et al, 2013;Carneiro et al, 2013). Conventional change one-factor-at-a-time approach has been applied to optimize the enzyme production using different medium constituents.…”

Section: Introductionmentioning

confidence: 99%

Statistical optimization of α-amylase production by Escherichia coliusing extruded bean as nitrogen and carbon source

Pereira¹,

Alves²,

Purcena³

et al. 2017

IJEAB

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“…Outros estudos demonstram, ainda, que o número de casos de morte violenta por suicídio tem aumentado consideravelmente [2][3][4][5]. O suicídio está relacionado a diversos fatores, sendo, em muitos casos, impossível descobrir sua causa determinante [6].…”

Section: Introductionunclassified

Sudorese post-mortem: relato de caso

Souza

Nascimento²,

Nasci³

et al. 2018

Rev. Bras. Crimin.

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ResumoO enforcamento é o meio mais adotado por homens e mulheres na prática do suicídio. Assim, o objetivo do presente estudo foi apresentar relato de um caso de suicídio por enforcamento, onde ocorreu processo de sudorese post-mortem em indivíduo adulto do sexo masculino. Os dados relatados foram extraídos de laudo elaborado por perito criminal do Instituto de Criminalística de Pernambuco Prof. Armando Samico. Além dos sinais clássicos previstos na literatura como relaxamento de esfíncteres e sulcos em torno do pescoço, chamou atenção a sudorese evidenciada após afrouxamento do laço horas após a morte, por ocasião do trabalho pericial. A ocorrência do fenômeno evidenciado deve ser investigada em mortes da mesma natureza e estudos devem ser conduzidos no sentido de melhor explicá-lo por se tratar de fenômeno raro para o qual inexiste uma explicação possível.Palavras-Chave: Asfixia; Sudorese; Suicídio. AbstractThe hanging is the most adopted means by men and women in the practice of suicide. Thus, the objective of this study was to report a suicide case by hanging, where the process of post-mortem sweating occurred in adult male sex. The reported data were extracted from a report prepared by a criminal expert from the Institute of Crime of Pernambuco Prof. Armando Samico. In addition to the classic signs predicted in the literature as relaxation of sphincters and furrows around the neck, drew attention to sweating evidenced after loosening the loop hours after death, on the occasion of forensic work. The occurrence of the phenomenon evidenced must be investigated in deaths of the same nature and studies should be conducted in order to better explain it because it is a rare phenomenon for which there is no possible explanation.Keywords: Asphyxiation; Sweating; Suicide. INTRODUÇÃONo Brasil estudo já realizado mostra que o enforcamento é o principal meio adotado em ato suicida utilizado por homens e mulheres [1]. Outros estudos demonstram, ainda, que o número de casos de morte violenta por suicídio tem aumentado consideravelmente [2][3][4][5]. O suicídio está relacionado a diversos fatores, sendo, em muitos casos, impossível descobrir sua causa determinante [6]. Alguns autores afirmam, por exemplo, existir relação entre a sazonalidade e ocorrência de suicídio [7]; outros, que o fenômeno em questão difere em relação aos gêneros e ao nível socioeconômico [8] e outros, ainda, que o suicídio é considerado um problema de saúde pública [9]; outros que o índice de suicídio na prisão é maior do que o na população em geral [10].Geralmente, em caso de suicídio por enforcamento, a literatura cita apenas os sinais clássicos que ocorrem no corpo da vítima, dentre os quais a sudorese ou hiperhidrose não faz parte. Sudorese ou hiperhidrose é um processo cuja principal característica é a transpiração em excesso ou profusa. O suor é um mecanismo que torna possível a adaptação do corpo as variações de temperatura a que o ser humano pode ser exposto. Em condições normais, as pessoas transpiram quando submetidas a calor

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Modeling microbial ethanol production by E. coli under aerobic/anaerobic conditions: Applicability to real postmortem cases and to postmortem blood derived microbial cultures

Cited by 34 publications

References 22 publications

Microbial forensics: new breakthroughs and future prospects

Microbial forensics: new breakthroughs and future prospects

Statistical optimization of α-amylase production by Escherichia coliusing extruded bean as nitrogen and carbon source

Sudorese post-mortem: relato de caso

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