Modeling and Predicting the Biofilm Formation of Different Salmonella Strains

Abstract

Biofilm formations of 25 Salmonella strains belonging to 19 serotypes were monitored with respect to time at a constant temperature. Two (DMC8 and DMC73) out of 25 strains had low biofilm formation ability and were not included in the modeling study. One (SL1344) strain had awkward biofilm formation which could not be described by a mathematical model, whereas biofilm formation of three strains (DMC33, DMC85 and DMC93) could be described by an exponential model. The modified Gompertz equation with high adjusted determination coefficient (R 2 adj) and low mean square error values produced reasonable fits for the biofilm formation of 19 Salmonella strains. It was also possible to predict the biofilm formation of these strains at two different time values by use of the modified Gompertz equation. Although more studies should be carried out to evaluate different mathematical models under both iso-and nonisothermal conditions, this study reveals that describing and predicting the biofilm formation could be possible.

Biofilm Forming Ability Of Salmonella Enteritidis In Vitro

“…Similar results were reported by Vestby et al [30]. In the research of Solano et al [22], a strong correlation between pellicle production and biofi lm-forming ability on polyvinyl chloride surface was detected, opposite to research of Karaca et al [28] who established a negative correlation between these tests.…”

“…The named authors investigated the infl uence of three different incubation temperatures (~ 22°C, 30°C and 37°C) on biofi lm formation by 30 isolates of S. enterica (S. Enteritidis n=29 and S. Typhimurium n=1) using TSB, and they reported that biofi lm formation occurred under all tested temperature conditions, but the highest biofi lm yield was obtained after 48 hours of incubation at ~ 22°C. Higher biofi lm yield at close to ambiental temperatures was also achieved in previous investigations [28,39].…”

Modeling and predicting the biofilm formation ofSalmonellaVirchow with respect to temperature and pH

“…4. As mentioned before Karaca et al [16] and Speranza et al [32] used the same equation to describe the biofilm formation; however, to the best of our knowledge this is the first study in which prediction of biofilm formation at different temperature and pH levels was performed.…”

“…Speranza et al [32] It could be also possible to use different equations as the secondary model to define the temperature and pH dependency of primary model parameters; however, polynomial equation produced the best goodness-of-fit among the alternatives (data not shown). Precautions such as cleaning and disinfection of the surface could be taken to avoid the biofilm formation before λ is reached [16] because if biofilms are formed by pathogenic microorganisms in the food environment, it is very difficult for them to be completely destroyed or removed from the food-processing facilities [30]. The λ values shown in Fig.…”

Mathematical Models for the Biofilm Formation of Geobacillus and Anoxybacillus on Stainless Steel Surface in Whole Milk

“…where A is the maximum cell number in Log 10 CFU/cm 2 attained during the stationary period, μ m is the maximum biofilm formation rate in Log 10 CFU/cm 2 ·h, and λ is the lag time in h. Although this model is widely used to describe the microbial growth curves, it could also be possible to use the modified Gompertz equation Eq. (3) to describe the biofilm formation of bacteria ( Karaca et al, 2013 ; Speranza et al, 2011 ).…”