Mode of Action and Cytotoxicity of Bioactive Compounds Produced by Streptomyces sp. KB1

Lertcanawanichakul, Monthon; Chawawisit, Kittisak

doi:10.17265/2328-2150/2019.09.002

Cited by 3 publications

(3 citation statements)

References 9 publications

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“…In present study constitutive Clindamycin reistance (cMLSB) rate was 15% which is accordance with Mokta et al1 17.14% and Lall et al27 16.6%. Higher percentage was reported by Pal et al22 46.9% where as lower percentage was observed in Patil et al28 3.55% and Mittal et al29 6.15%. In the present study 17.6% isolates showed true Clindamycin susceptibility (MS phenotype) which is similar to Patil et al28 15.33% and Mittal et al 29 15%.…”

mentioning

confidence: 69%

“…5 There is increasing frequency of MRSA infections and frequently changing antimicrobial resistance pattern make clinicians to jump on the macrolide lincosamidestreptogramin B (MLSB) antibiotics to treat infections. 6 One of the effective drug is Clindamycin which belongs to lincosamide group. The antibiotics belongs to MLSB family are chemically distinct but share a similar mode of action by binding to 23s rRNA -large ribosomal subunit and inhibit protein synthesis.…”

Section: Introductionmentioning

confidence: 99%

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Inducible Clindamycin resistance and MRSA amongst Staphylococcus aureus isolates: A phenotypic detection

Panwala¹,

Gandhi²,

Jethwa³

2021

IJMMTD

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Now a days clinicians switch over to drug Clindamycin to treat Staphylococcus aureus infections. Clindamycin is belonging to lincosamide group. As frequent use of this Clindamycin develops resistance among patients and ultimately treatment failure. Aim: This present research is done to identify type of resistance like inducible or constitutive macrolide lincosamide-streptogramin B (iMLSB /cMLSB) resistance and MS (Macrolide lincosamide streptogramin) phenotypes among Staphylococcus aureus isolated from various samples received in Microbiology laboratory of tertiary care hospital of south Gujarat. Materials and Methods: Among various samples total 232 Staphylococcus aureus were isolated. And all these isolates were subjected to routine antibiotic sensitivity testing by kirbey bauer disc diffusion method. Methicillin resistance staphylococcus aureus (MRSA) detected by using Cefoxitin disc. D test is performed as per Clinical and laboratory standards institute (CLSI) guidelines on all isolates. Results: Total of 232 Staphylococcus aureus were isolated, among them 109 were Methicillin sensitive Staphylococcus aureus (MSSA) and 123 were Methicillin resistant Staphylococcus aureus (MRSA). Prevalence of iMLSB, cMLSB and MS phenotype were 59.34% ,15.44% and 13% in MRSA while 12.84%, 14.67% and 22.93% respectively in MSSA. Conclusion: This research helps to detect Clindamycin resistance among Staphylococcus aureus and role of D test before starting the treatment with Clindamycin. By these knowledge clinician can choose correct treatment and we can prevent a treatment failure.

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mentioning

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Inducible Clindamycin resistance and MRSA amongst Staphylococcus aureus isolates: A phenotypic detection

Panwala¹,

Gandhi²,

Jethwa³

2021

IJMMTD

View full text Add to dashboard Cite

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“…These compounds exhibit a range of biological activities, including antifungal, antimicrobial, antibiofilm, antitumor, anticancer, insecticidal, and anthelmintic properties. They are also sources of industrially relevant enzymes [1–11]. A major challenge in harnessing the full potential of these compounds lies in the fact that many of them are not fully expressed under standard laboratory conditions in their native Streptomyces hosts.…”

Section: Introductionmentioning

confidence: 99%

Editing Streptomyces genome using target AID system fused with UGI‐degradation tag

Apriliana,

Kahar,

Kashiwagi

et al. 2024

Engineering in Life Sciences

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The utilization of Streptomyces as a microbial chassis for developing innovative drugs and medicinal compounds showcases its capability to produce bioactive natural substances. Recent focus on the clustered regularly interspaced short palindromic repeat (CRISPR) technology highlights its potential in genome editing. However, applying CRISPR technology in certain microbial strains, particularly Streptomyces, encounters specific challenges. These challenges include achieving efficient gene expression and maintaining genetic stability, which are critical for successful genome editing. To overcome these obstacles, an innovative approach has been developed that combines several key elements: activation‐induced cytidine deaminase (AID), nuclease‐deficient cas9 variants (dCas9), and Petromyzon marinus cytidine deaminase 1 (PmCDA1). In this study, this novel strategy was employed to engineer a Streptomyces coelicolor strain. The target gene was actVA‐ORF4 (SCO5079), which is involved in actinorhodin production. The engineering process involved introducing a specific construct [pGM1190‐dcas9‐pmCDA‐UGI‐AAV‐actVA‐ORF4 (SCO5079)] to create a CrA10 mutant strain. The resulting CrA10 mutant strain did not produce actinorhodin. This outcome highlights the potential of this combined approach in the genetic manipulation of Streptomyces. The failure of the CrA10 mutant to produce actinorhodin conclusively demonstrates the success of gene editing at the targeted site, affirming the effectiveness of this method for precise genetic modifications in Streptomyces.

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Isolation of Antimicrobial Producing Actinomycetes from Indigenous Microhabitats.

Aku

Alisigwe

Ezenwosu³

et al. 2022

PBMJ

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Unusual microhabitats in different locations present opportunities for the bio-prospecting of actinomycetes with high antimicrobial producing potentials. A total of 113 actinomycetes from specific indigenous microhabitats were isolated and screened for their antimicrobial activity against selected test organisms. The earthworm castings, forest litter, and domestic dumpsite sites, respectively, accounted for approximately 24.78%, 19.47%, and 18.5% of the total number of isolates recovered. The preliminary antagonism assay using the perpendicular streak method yielded four isolates that showed high antimicrobial activity and broad spectrum activity against test bacteria or fungi. These isolates showed excellent growth and sporulation on milk agar and starch casein agar, and the micro-morphological characterization of the isolates revealed the presence of spore-bearing aerial hyphae. Bioactive extracts from DS15 and EC5, at 125 mg/mL, had respective zones of inhibition measuring 20.7 ± 0.6 and 33.0 ± 1.0 against multidrug resistant Escherichia coli (clinical isolate) and Salmonella typhi (ATCC 2923) respectively. Bioactive extracts from EC1 and EC9, at 125 mg/mL, show respective zones of inhibition measuring 18.7 ± 1.2 and 16.7 ± 1.2 against Candida albicans (ATCC 10231) and C. glabrata (ATCC 22018) respectively. This study reinforces the evidence that unique and unusual microhabitats, in different geographical locations, present an excellent opportunity for the bio prospecting of actinomycetes with high antimicrobial producing potentials. Furthermore, it shows that the indigenous antagonistic actinomycete isolates exhibited potent antimicrobial activity against test bacteria and fungi employed in the in-vitro antimicrobial assays.

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Mode of Action and Cytotoxicity of Bioactive Compounds Produced by Streptomyces sp. KB1

Cited by 3 publications

References 9 publications

Inducible Clindamycin resistance and MRSA amongst Staphylococcus aureus isolates: A phenotypic detection

Inducible Clindamycin resistance and MRSA amongst Staphylococcus aureus isolates: A phenotypic detection

Editing Streptomyces genome using target AID system fused with UGI‐degradation tag

Isolation of Antimicrobial Producing Actinomycetes from Indigenous Microhabitats.

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