MMP-9 deficiency affects axonal outgrowth, migration, and apoptosis in the developing cerebellum

Vaillant, Catherine; Meissirel, Claire; Mutin, M; Belin, Marie‐Françoise; Lund, Leif R.; Thomasset, Nicole

doi:10.1016/s1044-7431(03)00196-9

Cited by 127 publications

(112 citation statements)

References 60 publications

Supporting

Mentioning

105

Contrasting

Order By: Relevance

“…These results received in the studies of ocular cells and tissues are in a good agreement with the data about the association of neuronal apoptosis with increased MMP-9 activity in the central nervous system [17,18] suggesting a more general function of MMP-9 modulating apoptosis in neuronal tissues and in glia-neurons interaction. MMP-9 is considered one of the pharmacological targets in the treatment of glaucoma [15] and other diseases [19][20][21][22].…”

Section: Introductionsupporting

confidence: 88%

Matrix metalloproteinase 9 expression: new regulatory elements

Surgucheva

Chidambaram

Willoughby

et al. 2010

j ocul biol dis inform

View full text Add to dashboard Cite

Retinal ganglion cells apoptosis is linked to matrix metalloproteinase 9 (MMP-9) controlled changes of extracellular matrix. Abnormal expression of MMP-9 is associated with glaucomatous alterations. Thus, the knowledge of MMP-9 regulation is important for the understanding the pathogenesis of glaucoma. Here, we investigated the role of 3′-untranslated regions (3′-UTR) and microRNAs in MMP-9 regulation. We used in vitro mutagenesis and Luc reporter system to identify regulatory elements in the 3′-UTR of MMP-9. microRNAs were analyzed by qRT-PCR, and their role was investigated with inhibitors and mimics. We identified targets for miRNAs in 3′-UTR of MMP-9 involved in the regulation of MMP-9 expression. We then isolated miRNAs from the optic nerve A7 astrocytes and 293 T cells and confirmed the role of mi340 in the regulation using specific inhibitors and mimics. The results obtained show a new miRNA-mediated mechanism of MMP-9 expression regulation.

show abstract

Section: Introductionsupporting

confidence: 88%

Matrix metalloproteinase 9 expression: new regulatory elements

Surgucheva

Chidambaram

Willoughby

et al. 2010

j ocul biol dis inform

View full text Add to dashboard Cite

show abstract

“…The link between MMP-9 and apoptosis has been shown only in the cardiac cells of diabetic mice and for neuronal growth. 49,50 In these studies, treatment of IL-6 Ϫ/Ϫ animals with MMP-2/9 inhibitors also resulted in a decrease in apoptosis via TUNEL staining. 17 More specific studies investigating the mechanisms involved in this model are not yet available.…”

Section: Discussionmentioning

confidence: 94%

Matrix metalloproteinase-9 is an important factor in hepatic regeneration after partial hepatectomy in mice

et al. 2006

View full text Add to dashboard Cite

Partial hepatectomy triggers hepatocyte proliferation, hepatic matrix remodeling, and hepatocyte apoptosis, all of which are important processes in the regenerating liver. Previous studies have shown an increase in the levels of matrix metalloproteinases gelatinase A (MMP-2) and gelatinase B (MMP-9) after partial hepatectomy. The goal of this study was to investigate the role of MMP-9 in liver regeneration after partial hepatectomy. A 70% hepatectomy or sham laparotomy was performed in wild-type or MMP-9 -deficient (MMP-9 ؊/؊ ) mice. Hepatic regeneration was determined by liver weight/total body weight ratios and BrdU staining, which was used to a calculate mitotic index at several times postoperatively. Cytokine and growth factor expression was evaluated by Luminex™ bead-based ELISA and Western blots. Finally, the effect of MMP-9 on apoptosis was measured using TUNEL and caspase expression. The MMP-9 ؊/؊ animals had a delayed hepatic regenerative response when compared with wild-type controls. The MMP-9 -deficient animals expressed significantly less VEGF, HGF, and TNF-␣ between days 2 and 3 post-hepatectomy. Apoptosis, as measured by TUNEL staining and caspase expression, was decreased in the MMP-9 ؊/؊ . In conclusion, MMP-9 plays an important role in liver regeneration after partial hepatectomy by affecting matrix remodeling, as well as cytokine, growth factor, and caspase expression.

show abstract

“…In recent years, metalloproteinase activity has been correlated with many aspects of development. Examples include the observations that angiogenesis (Canete Soler et al, 1995a;Vu et al, 1998;Werb et al, 1999) and the migration of neuronal precursor cells and OPCs (Amberger et al, 1997;Vaillant et al, 2003) seem to require metalloproteinase activity. A growing list has ascribed novel functions of metalloproteinases in normal development, such as in cytokine activation-inactivation and controlling apoptotic pathways (for review, see Sternlicht and Werb, 2001).…”

Section: Discussionmentioning

confidence: 99%

The Expression of Matrix Metalloproteinase-12 by Oligodendrocytes Regulates Their Maturation and Morphological Differentiation

Larsen¹,

Yong²

2004

J. Neurosci.

View full text Add to dashboard Cite

Matrix metalloproteinases (MMPs) have important roles in many processes of the developing CNS requiring proteolytic activity such as the migration of neuronal precursors, axonal outgrowth, and vascularization. Another developmental event involving proteolysis is myelin formation, whereby the extensive processes elaborated from oligodendrocytes (OLs) enwrap axons. Here we find MMP-12 transcripts to be produced by OLs in much higher levels than other MMP members examined. MMP-12 activity correlated with the ability of OLs to extend processes in vitro, suggesting a role for MMP-12 in the morphological differentiation of OLs. This was corroborated by results that OL lineage cells from MMP-12 null mice were retarded in their ability to differentiate morphologically and that this deficiency was overcome by the exogenous addition of active MMP-12. Finally, the maturation of oligodendrocyte precursor cells (OPCs) to OLs was significantly reduced in cultures from MMP-12 null mice compared with wild-type controls. We conclude that OL lineage cells express MMP-12 during their maturation and that MMP-12 activity has functional involvement both in maturation of OPCs and in the ability of OPCs and OLs to extend processes.

show abstract

MMP-9 deficiency affects axonal outgrowth, migration, and apoptosis in the developing cerebellum

Cited by 127 publications

References 60 publications

Matrix metalloproteinase 9 expression: new regulatory elements

Matrix metalloproteinase 9 expression: new regulatory elements

Matrix metalloproteinase-9 is an important factor in hepatic regeneration after partial hepatectomy in mice

The Expression of Matrix Metalloproteinase-12 by Oligodendrocytes Regulates Their Maturation and Morphological Differentiation

Contact Info

Product

Resources

About