MMP-2 and MMP-9 in normal mucosa are independently associated with outcome of colorectal cancer patients

Langers, Alexandra M. J.; Verspaget, Hein W.; Hawinkels, Lukas J.A.C.; Kubben, F. J. G. M.; Duijn, Wim van; Reijden, Johan J. van der; Hardwick, James C.H.; Hommes, Daan W.; Sier, Cornelis F.M.

doi:10.1038/bjc.2012.80

Cited by 69 publications

(56 citation statements)

References 18 publications

(20 reference statements)

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“…Much evidence has linked MMPs to aggressive, malignant behavior, and poor outcome in various cancer cells, including prostate cancer, breast cancer, lung cancer et al They remodel the extracellular matrix and increase the invasive potential of tumor cells. The expression level is associated with tumor invasion ability and survival (Quaranta et al, 2007;Chen et al, 2012;Irena et al, 2012;Langers et al, 2012;Dai et al, 2013). Our study showed mIDH1 reduced the invasion ability of glioma cells and was further reduced by TMZ.…”

Section: Discussionsupporting

confidence: 53%

IDH1 Overexpression Induced Chemotherapy Resistance and IDH1 Mutation Enhanced Chemotherapy Sensitivity in Glioma Cells in Vitro and in Vivo

Wang¹,

Dong²,

Wang³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Isocitrate dehydrogenase (IDH) is of great importance in cell metabolism and energy conversion. IDH mutation in glioma cells is reported to be associated with an increased overall survival. However, effects biological behavior of therapy of gliomas are unclear. Here, we investigated the influence of wild-type and mutated IDH genes on glioma cell biological behavior and response to chemotherapy. Relevant mechanisms were further explored. We designed our study on the background of the IDHR132H mutation. Stable cell lines were constructed by transfection. The CCK-8 method was used to assess cell proliferation, flow cytometry for the cell cycle and cell apoptosis, and the transwell method for cell invasion. Nude mouse models were employed to determine tumorigenesis and sensitivity to chemotherapy. Western blotting was used to detect relevant protein expression levels. We found that overexpression of wild IDH1 gene did not cause changes in the cell cycle, apoptosis and invasion ability. However, it resulted in chemotherapy resistance to a high dose of temozolomide (TMZ) in vivo and in vitro. The IDH1 mutation caused cell cycle arrest in G1 stage and a reduction of proliferation and invasion ability, while raising sensitivity to chemotherapy. This may provide an explanation for the better prognosis of IDH1 mutated glioma patients and the relative worse prognosis of their wild-type IDH1 counterparts. We also expect IDH1 mutations may be optimized as new targets to improve the prognosis of glioma patients.

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Section: Discussionsupporting

confidence: 53%

IDH1 Overexpression Induced Chemotherapy Resistance and IDH1 Mutation Enhanced Chemotherapy Sensitivity in Glioma Cells in Vitro and in Vivo

Wang¹,

Dong²,

Wang³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…Langers et al (Langers et al, 2012) have found that high expression of MMP-2 and MMP-9 in the mucosa of colon cancer patients is associated with a 5-year mean survival (Grigioni et al, 1994;Sier et al, 1996). In this study, we found similar results regarding MMP-2 and MMP-9 expression in EGISTs, i.e., the expression of MMP-2 and MMP-9 was associated with tumor progression, which is consistent with previous findings (Grigioni et al, 1994;Sier et al, 1996;Zeng et al, 1996;Ring et al, 1997;Yukawa et al, 2001;Zhang et al, 2003;Liu et al, 2010;Langers et al, 2012). However, our current study did not show the underlying molecular mechanisms responsible for upregulation of MMP-2 and MMP-9 in EGISTs or how MMP-2 and MMP-9 proteins promote EGIST progression.…”

Section: Discussionmentioning

confidence: 99%

Association of Matrix Metalloproteinase (MMP)-2 and -9 Expression with Extra-gastrointestinal Stromal Tumor Metastasis

Wang¹,

Ma²,

Jin³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Matrix metalloproteinase (MMP)-2 and MMP-9 are important proteases involved in invasion and metastasis of various tumors. Extra-gastrointestinal stromal tumors (EGISTs) are rare neoplasms. This study was performed to assess MMP-2 and MMP-9 expression in EGIST tissue samples for association with clinicopathological data from the patients. Twenty-one surgical EGIST tissue specimens were collected for analysis of MMP-2 and MMP-9 expression using immunohistochemistry. MMP-2 and MMP-9 proteins were expressed in all of the epithelial cell types of EGISTs, whereas they were only expressed in 75% of the spindle cell type, although there was no statistically significant difference (p>0.05). Expression of MMP-2 and MMP-9 proteins was associated with tumor size, mitotic rate, tumor necrosis, and distant metastasis (p<0.05). MMP-2 expression was linked with MMP-9 levels (p<0.05). However, there was no correlation between MMP-9 expression and age, sex, primary site, or cell morphology in any of these 21 EGIST patients (p>0.05). Moreover, expression of MMP-2 and MMP-9 proteins increased with the degree of EGIST risk. This study provided evidence of an association of MMP-2 and MMP-9 expression with advanced EGIST behavior.Keywords: Extra-gastrointestinal stromal tumor (EGIST) -MMP-2 -MMP-9 -tumor invasion and metastasis -biomarker

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“…In CRC patients, plasma MMP-2 levels are correlated with disease stage and survival (19), while MMP-9 expression is correlated with the progression of CRC and hence may be a potential target for the treatment of CRC (20,21). Furthermore, MMP-2 and MMP-9 expression in normal mucosa from patients with CRC are independently associated with the outcome of patients with CRC (22). Therefore, to elucidate the mechanism of the antimetastatic effect of baicalein, the present study determined the expression levels of MMP-2/-9 in CRC cells treated with baicalein, and baicalein was demonstrated to significantly inhibit the protein expression levels of MMP-2/-9 in CRC cells.…”

Section: Discussionmentioning

confidence: 99%

Baicalein inhibits the migration and invasion of colorectal cancer cells via suppression of the AKT signaling pathway

2015

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Abstract. The anticancer effect of baicalein has been known for a number of years. However, its anti-metastatic effect and associated mechanisms in colorectal cancer (CRC) remain unclear. The present study investigated the hypothesis that treatment with baicalein may inhibit the proliferation, motility and invasion of human CRC cell lines via regulation of the protein kinase B (AKT) signaling pathway. Baicalein was demonstrated to significantly inhibit the migration and invasion of CRC cells (P=0.01). Additionally, after treatment with baicalein for 24 h, the protein expression levels of matrix metalloproteinase-2 (MMP-2) and MMP-9 in CRC cells were significantly reduced in a dose-dependent manner (P=0.01). Furthermore, treatment with baicalein significantly reduced the expression levels of phosphorylated AKT (P=0.01). In conclusion, baicalein appears to inhibit CRC cell migration and invasion by reducing the expression of MMP-2 and MMP-9 via suppression of the AKT signaling pathway. Thus, baicalein is a potential novel therapeutic agent for patients with CRC.

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MMP-2 and MMP-9 in normal mucosa are independently associated with outcome of colorectal cancer patients

Cited by 69 publications

References 18 publications

IDH1 Overexpression Induced Chemotherapy Resistance and IDH1 Mutation Enhanced Chemotherapy Sensitivity in Glioma Cells in Vitro and in Vivo

IDH1 Overexpression Induced Chemotherapy Resistance and IDH1 Mutation Enhanced Chemotherapy Sensitivity in Glioma Cells in Vitro and in Vivo

Association of Matrix Metalloproteinase (MMP)-2 and -9 Expression with Extra-gastrointestinal Stromal Tumor Metastasis

Baicalein inhibits the migration and invasion of colorectal cancer cells via suppression of the AKT signaling pathway

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