Mitogen-activated protein kinases, Fus3 and Kss1, regulate chronological lifespan in yeast

Aluru, Maneesha; McKinney, Tori; Venero, Anne-Kathryn L.; Choudhury, Shilpa; Torres, Matthew P.

doi:10.18632/aging.101350

Cited by 9 publications

(5 citation statements)

References 75 publications

(118 reference statements)

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“…S. cerevisiae encodes seven phosphatases in the type 2C Ser/Thr phosphatase (PP2C) superfamily (Ptc1-Ptc7) ( Ariño et al, 2011 ), and Ptc1, Ptc2, and Ptc3 are negative regulators of MAPK ( Warmka et al, 2001 ). In deficiencies of the MAPK pathway, cells elevate their stress tolerance ability to extend CLS ( Aluru et al, 2017 ). Although currently there is no direct evidence that PP2C may control stress tolerance through the MAPK pathway, our study provides a means by which PP2C governs stress tolerance: PP2C removes PKA-mediated Ids2 phosphorylation to maintain ample chaperone activity.…”

Section: Discussionmentioning

confidence: 99%

Glucose intake hampers PKA-regulated HSP90 chaperone activity

Chen

Jiang

Chen

et al. 2018

eLife

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Aging is an intricate phenomenon associated with the gradual loss of physiological functions, and both nutrient sensing and proteostasis control lifespan. Although multiple approaches have facilitated the identification of candidate genes that govern longevity, the molecular mechanisms that link aging pathways are still elusive. Here, we conducted a quantitative mass spectrometry screen and identified all phosphorylation/dephosphorylation sites on yeast proteins that significantly responded to calorie restriction, a well-established approach to extend lifespan. Functional screening of 135 potential regulators uncovered that Ids2 is activated by PP2C under CR and inactivated by PKA under glucose intake. ids2Δ or ids2 phosphomimetic cells displayed heat sensitivity and lifespan shortening. Ids2 serves as a co-chaperone to form a complex with Hsc82 or the redundant Hsp82, and phosphorylation impedes its association with chaperone HSP90. Thus, PP2C and PKA may orchestrate glucose sensing and protein folding to enable cells to maintain protein quality for sustained longevity.

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Section: Discussionmentioning

confidence: 99%

Glucose intake hampers PKA-regulated HSP90 chaperone activity

Chen

Jiang

Chen

et al. 2018

eLife

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“…In the model fungus, Saccharomyces cerevisiae , five important cellular development events, namely sporulation, cell wall integrity, osmoregulation, sexual mating, and filamentous (pseudohyphal) growth, have been shown to be regulated respectively by five established MAPK pathways ( Chen and Thorner, 2007 ; Vallejo and Mayinger, 2015 ). Specifically, S. cerevisiae Hog1 is integral to the osmoregulatory signal transduction cascade ( Nancy et al, 2015 ), Fus3/Kss1 are essential for sexual reproduction and regulate chronological life span ( Maneesha et al, 2017 ), and MPK1 is involved in cell wall integrity ( Kim et al, 2018 ). In addition, a specialized type of MAPKs in fungi and mammalian was termed as stress-activated protein kinases (SAPKs), as it is responsible for stress response, including heat shock, hyperosmolarity, ultraviolet (UV) light irradiation, and oxidative stress ( Brown et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

PlMAPK10, a Mitogen-Activated Protein Kinase (MAPK) in Peronophythora litchii, Is Required for Mycelial Growth, Sporulation, Laccase Activity, and Plant Infection

et al. 2018

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Mitogen-activated protein kinase (MAPK) pathways are ubiquitous and evolutionarily conserved signal transduction modules directing cellular respond to a diverse array of stimuli, in the eukaryotic organisms. In this study, PlMAPK10 was identified to encode a MAPK in Peronophythora litchii, the oomycete pathogen causing litchi downy blight disease. PlMAPK10, containing a specific and highly conserved dual phosphorylation lip sequence SEY (Serine-Glutamic-Tyrosine), represents a novel group of MAPKs as previously reported. Transcriptional profiling showed that PlMAPK10 expression was up-regulated in zoospore and cyst stages. To elucidate its function, the PlMAPK10 gene was silenced by stable transformation. PlMAPK10 silence did not impair oospore production, sporangium germination, zoospore encyst, or cyst germination but hindered hyphal growth, sporulation, pathogenicity, likely due to altering laccase activity. Over all, our results indicated that a MAPK encoded by PlMAPK10 gene in P. litchii is important for pathogenic development.

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“…This negative regulator role of Ptc4 may contribute to rapid cell proliferation. Moreover, deletion of HOG1 in S. cerevisiae extends CLS and confers thermal and oxidative stress tolerances 49 , 58 , suggesting that inactivation of Hog1 through dephosphorylation by Ptc4 contributes to CLS extension and stress tolerance for long-term cell survival. In the case of Zwf1, its overexpression may enhance the pentose phosphate pathway to generate reduced nicotinamide adenine dinucleotide phosphate (NADPH) 59 , 60 .…”

Section: Discussionmentioning

confidence: 99%

Screening of selected ageing-related proteins that extend chronological life span in yeast Saccharomyces cerevisiae

Lee¹,

Ong²,

Ong

et al. 2021

Sci Rep

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Ageing-related proteins play various roles such as regulating cellular ageing, countering oxidative stress, and modulating signal transduction pathways amongst many others. Hundreds of ageing-related proteins have been identified, however the functions of most of these ageing-related proteins are not known. Here, we report the identification of proteins that extended yeast chronological life span (CLS) from a screen of ageing-related proteins. Three of the CLS-extending proteins, Ptc4, Zwf1, and Sme1, contributed to an overall higher survival percentage and shorter doubling time of yeast growth compared to the control. The CLS-extending proteins contributed to thermal and oxidative stress responses differently, suggesting different mechanisms of actions. The overexpression of Ptc4 or Zwf1 also promoted rapid cell proliferation during yeast growth, suggesting their involvement in cell division or growth pathways.

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Mitogen-activated protein kinases, Fus3 and Kss1, regulate chronological lifespan in yeast

Cited by 9 publications

References 75 publications

Glucose intake hampers PKA-regulated HSP90 chaperone activity

Glucose intake hampers PKA-regulated HSP90 chaperone activity

PlMAPK10, a Mitogen-Activated Protein Kinase (MAPK) in Peronophythora litchii, Is Required for Mycelial Growth, Sporulation, Laccase Activity, and Plant Infection

Screening of selected ageing-related proteins that extend chronological life span in yeast Saccharomyces cerevisiae

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