Mitochondrial and caspase pathways are involved in the induction of apoptosis by nardosinen in MCF-7 breast cancer cell line

Shahali, Amirhosein; Ghanadian, Mustafa; Jafari, Seyyed Mehdi; Aghaei, Mahmoud

doi:10.4103/1735-5362.220963

Cited by 26 publications

(16 citation statements)

References 36 publications

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“…Natural products extracted from plants could provide novel anticancer drugs (Shahali et al, 2018[ 30 ]). Likewise, our study presents the invasive plant; Prosopis juliflora, as a natural source of anticancer chemical compounds, i.e.…”

Section: Discussionmentioning

confidence: 99%

Prosopis juliflora leave extracts induce cell death of MCF-7, HepG2, and LS-174T cancer cell lines

Elbehairi

Ahmed

Alshati

et al. 2020

EXCLI Journal; 19:Doc1282; ISSN 1611-2156

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Section: Discussionmentioning

confidence: 99%

Prosopis juliflora leave extracts induce cell death of MCF-7, HepG2, and LS-174T cancer cell lines

Elbehairi

Ahmed

Alshati

et al. 2020

EXCLI Journal; 19:Doc1282; ISSN 1611-2156

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“…Apoptosis is a critical homeostatic mechanism involved in anti-cancer drug-induced proliferation inhibition (35). The mitochondrial apoptotic pathway has a critical role in drug-mediated apoptosis and is often regarded as a potential anti-cancer target (39–41). Bcl-2 family protein members, including Bax and Bcl-2, serve important roles in the mitochondrial apoptotic pathway.…”

Section: Discussionmentioning

confidence: 99%

Alantolactone induces gastric cancer BGC‑823 cell apoptosis by regulating reactive oxygen species generation and the AKT signaling pathway

Zhang

2019

Oncol Lett

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Alantolactone (ALT), a natural sesquiterpene lactone, has been suggested to exert anti-cancer activities in various cancer cell lines. However, the effects and mechanisms of action of ALT in human gastric cancer remains to be elucidated. In the present study, the effects of ALT on BGC-823 cells were examined and the underlying molecular mechanisms associated with these effects were investigated. Cell viability was detected by using an MTT assay. Cell cycle, cell apoptosis and the level of reactive oxygen species (ROS) were assessed by flow cytometry, and the expression levels of proteins of interest were analyzed by western blot assay. The results demonstrated that ALT triggered apoptosis and induced G0/G1 phase arrest in a dose-dependent manner. Furthermore, the expression level of the anti-apoptosis protein Bcl-2 was downregulated, and expression of the pro-apoptosis proteins Bax and cleaved PARP were significantly upregulated. The cell cycle-associated proteins cyclin-dependent kinase inhibitor 1 and cyclin-dependent kinase inhibitor 1B were also increased, while cyclin D1 was deceased. In addition, ALT induced apoptosis via the inhibition of RAC-alpha serine/threonine-protein kinase (AKT) signaling and ROS generation, which was effectively inhibited by the ROS scavenger, N-acetyl cysteine. Therefore, the results from the present study indicated that the ROS-mediated inhibition of the AKT signaling pathway serves an important role in ALT-induced apoptosis in BGC-823 cells. In conclusion, the results demonstrated that ALT exerted significant anti-cancer effects against gastric cancer cells in vitro .

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“…Apoptosis was performed by flow cytometry as described previously(1819). In brief, cells were seeded and incubated with different concentration of Gal-9 (1, 10, 50, and 100 nM).…”

Section: Methodsmentioning

confidence: 99%

Galectin-9 induces apoptosis in OVCAR-3 ovarian cancer cell through mitochondrial pathway

et al. 2018

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Galectin-9 (Gal-9), a member of animal lectins' family, is implicated in the induction of apoptosis in various cancer cells. Here, we evaluated the anti-tumor effect of Gal-9 in OVCAR-3 ovarian cancer cells. The effect of the Gal-9 on cell viability was evaluated using MTT assays. Apoptosis was assessed using Annexin-V staining. The assessment of mitochondrial membrane potential (ΔΨm) was performed using a JC-1 probe. The activity of caspase-3 and caspase-6 were evaluated with colorimetric assay. The production of reactive oxygen species (ROS) was applied by fluorescent probe. The expression levels of Bax and Bcl-2 were assessed using western blotting. The result showed that Gal-9 inhibits cell viability. Flow cytometry analysis showed that Gal-9 induces apoptosis in ovarian cancer cells. Moreover, Gal-9 decreased ΔΨm and increased the generation of ROS and caspase-3 and caspase-6 activities in ovarian cancer cells. Moreover, Gal-9 induced expression of Bax as well as inhibited expression of Bcl-2. In conclusion, our results indicated that Gal-9 induced apoptosis in ovarian cancer cells through mitochondrial pathway.

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Mitochondrial and caspase pathways are involved in the induction of apoptosis by nardosinen in MCF-7 breast cancer cell line

Cited by 26 publications

References 36 publications

Prosopis juliflora leave extracts induce cell death of MCF-7, HepG2, and LS-174T cancer cell lines

Prosopis juliflora leave extracts induce cell death of MCF-7, HepG2, and LS-174T cancer cell lines

Alantolactone induces gastric cancer BGC‑823 cell apoptosis by regulating reactive oxygen species generation and the AKT signaling pathway

Galectin-9 induces apoptosis in OVCAR-3 ovarian cancer cell through mitochondrial pathway

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