Mite Allergen Induces Allergic Dermatitis with Concomitant Neurogenic Inflammation in Mouse

Huang, Chiung Hui; Kuo, I-Chun; Xu, Hui; Lee, Yoke Sun; Chua, Kaw Yan

doi:10.1046/j.1523-1747.2003.12356.x

Cited by 59 publications

(52 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our data are reminiscent to previous studies in human AD showing that, although CD4 ϩ T cells predominated in AD skin lesions, T cells infiltrating the epidermis were almost exclusively CD8 ϩ T cells (12). Along these lines, studies in mouse models of AD reported that allergen challenge is associated with epidermal recruitment of CD8 ϩ T cells (25), which is dramatically enhanced by coexposure to the superantigen staphylococcal enterotoxin B (26). However, only a few CD8 ϩ T cells, compared with CD4 ϩ T cells, were detected in the skin in these studies probably because of the time at which T cell infiltration was analyzed.…”

Section: Discussionsupporting

confidence: 86%

Skin-Infiltrating CD8+ T Cells Initiate Atopic Dermatitis Lesions

Hennino

Vocanson

Toussaint

et al. 2007

The Journal of Immunology

103

View full text Add to dashboard Cite

Skin lesions in the allergic form of atopic dermatitis (AD) are induced by allergen-specific T cells that infiltrate the skin at the site of allergen exposure. Although Th2-type CD4+ T cells appear to be crucial in AD pathophysiology, little is known about the contribution of CD8+ T cells in the development of the allergic skin inflammation. In the present study, we have analyzed the respective role of CD8+ and CD4+ T cells in the development of AD skin lesions in a mouse model of allergen-induced AD. In sensitized mice, CD8+ T cells are rapidly and transiently recruited to the allergen-exposed site and initiate the inflammatory process leading to skin infiltration with eosinophils and Th1/Th2-producing cells. CD8+ T cell-depleted mice show no inflammation, demonstrating that these cells are mandatory for the development of AD. In contrast, CD4+ T cell-depleted mice develop a severe form of eczema. Furthermore, adoptive transfer of CD8+ T cells from sensitized mice into naive recipient mice leads to skin inflammation soon after allergen exposure. These data indicate that allergen-primed CD8+ T cells are required for the development of AD-like lesions in mice.

show abstract

Section: Discussionsupporting

confidence: 86%

Skin-Infiltrating CD8+ T Cells Initiate Atopic Dermatitis Lesions

Hennino

Vocanson

Toussaint

et al. 2007

The Journal of Immunology

103

View full text Add to dashboard Cite

show abstract

“…HDM is a common allergen associated with human AD and bronchial asthma (4,40). In the present study, we demonstrated that HDM-treated mice exhibited phenotypes reminiscent of both acute and chronic AD lesions (Figure 1 and Supplemental Figures 1 and 2), as previously reported (27,28). In light of these prior reports, we here adopted HDM-sensitized mice as a representative mouse model of allergic skin inflammation.…”

Section: Discussionsupporting

confidence: 83%

“…However, in our HDM-sensitized mouse model, enhancement of IFN-γ expression in skin tissues was not observed, despite the appearance of other features of chronic AD skin lesions (Figure 1 and data not shown). Huang et al have also reported that skin lesions in HDM-treated mice do not show IFN-γ expression, whereas splenocytes responsive to allergen stimulation can induce IFN-γ production (28). Exposure to microbial products may make the difference between mouse models and patients (4).…”

Section: Discussionmentioning

confidence: 99%

“…It has previously been reported that this mouse model exhibits phenotypes reminiscent of both acute and chronic AD lesions, including fibrosis, epidermal hyperplasia, spongiosis, lichenification, excoriation, and neurogenic inflammation (27,28). We confirmed that mice epicutaneously sensitized with HDM exhibited ear swelling and redness, hyperplasia and dysregulated differentiation of the epidermis, and dermal fibrosis ( Figure 1, A-C, and Supplemental Figure 1, A-D; supplemental material available online with this article; doi:10.1172/JCI58978DS1).…”

Section: Epicutaneous Sensitization With Hdm Induces Allergic Skin Inmentioning

confidence: 99%

See 1 more Smart Citation

Periostin promotes chronic allergic inflammation in response to Th2 cytokines

et al. 2012

View full text Add to dashboard Cite

Allergic inflammation triggered by exposure of an allergen frequently leads to the onset of chronic inflammatory diseases such as atopic dermatitis (AD) and bronchial asthma. The mechanisms underlying chronicity in allergic inflammation remain unresolved. Periostin, a recently characterized matricellular protein, interacts with several cell surface integrin molecules, providing signals for tissue development and remodeling. Here we show that periostin is a critical mediator for the amplification and persistence of allergic inflammation using a mouse model of skin inflammation. Th2 cytokines IL-4 and IL-13 stimulated fibroblasts to produce periostin, which interacted with α v integrin, a functional periostin receptor on keratinocytes, inducing production of proinflammatory cytokines, which consequently accelerated Th2-type immune responses. Accordingly, inhibition of periostin or α v integrin prevented the development or progression of allergen-induced skin inflammation. Thus, periostin sets up a vicious circle that links Th2-type immune responses to keratinocyte activation and plays a critical role in the amplification and chronicity of allergic skin inflammation.

show abstract

“…Previous reports have shown that few CD8 ϩ T cells are found in the epidermis of AD patients but not in the epidermis of healthy controls (26). Furthermore, Huang et al (27) reported recently that epicutaneous exposure to mite allergen induces allergic dermatitis and the infiltration of few CD8 ϩ T cells into the epidermis. Similar with our OVA sensitization, both of these earlier observations show only few numbers of CD8 ϩ T cells in the epidermis.…”

Section: Figure 2 Immunohistochemical Analysis Of Cd3mentioning

confidence: 97%

Topical Superantigen Exposure Induces Epidermal Accumulation of CD8+ T Cells, a Mixed Th1/Th2-Type Dermatitis and Vigorous Production of IgE Antibodies in the Murine Model of Atopic Dermatitis

Savinko

Lauerma²,

Lehtimäki

et al. 2005

The Journal of Immunology

View full text Add to dashboard Cite

Patients with atopic dermatitis (AD) have repeated cutaneous exposure to both environmental allergens and superantigen-producing strains of Staphylococcus aureus. We used a murine model of AD to investigate the role of staphylococcal enterotoxin B (SEB) in the modulation of allergen-induced skin inflammation. Mice were topically exposed to SEB, OVA, a combination of OVA and SEB (OVA/SEB), or PBS. Topical SEB and OVA/SEB exposure induced epidermal accumulation of CD8+ T cells and TCRVβ8+ cells in contrast to OVA application, which induced a mainly dermal infiltration of CD4+ cells. SEB and OVA/SEB exposure elicited a mixed Th1/Th2-associated cytokine and chemokine expression profile within the skin. Restimulation of lymph node cells from OVA- and OVA/SEB-exposed mice with OVA elicited strong production of IL-13 protein, whereas substantial amounts of IFN-γ protein were detected after SEB stimulation of cells derived from SEB- or OVA/SEB-exposed mice. Topical SEB treatment elicited vigorous production of SEB-specific IgE and IgG2a Abs and significantly increased the production of OVA-specific IgE and IgG2a Abs. The present study shows that topical exposure to SEB provokes epidermal accumulation of CD8+ T cells, a mixed Th2/Th1 type dermatitis and vigorous production of specific IgE and IgG2a Abs, which can be related to the chronic phase of atopic skin inflammation.

show abstract

Mite Allergen Induces Allergic Dermatitis with Concomitant Neurogenic Inflammation in Mouse

Cited by 59 publications

References 29 publications

Skin-Infiltrating CD8+ T Cells Initiate Atopic Dermatitis Lesions

Skin-Infiltrating CD8+ T Cells Initiate Atopic Dermatitis Lesions

Periostin promotes chronic allergic inflammation in response to Th2 cytokines

Topical Superantigen Exposure Induces Epidermal Accumulation of CD8+ T Cells, a Mixed Th1/Th2-Type Dermatitis and Vigorous Production of IgE Antibodies in the Murine Model of Atopic Dermatitis

Contact Info

Product

Resources

About