Mistyping of Angiotensinogen M235T Alleles

Lizanecz, Erzsébet; Pásztor, Enikő T.; Mohácsi, Attila; Papp, Zoltán; Édes, István; Tóth, Attila

doi:10.1291/hypres.29.197

Cited by 9 publications

(9 citation statements)

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“…In addition, misclassification of the AGT (M235T) genotype should also be considered, as has been indicated recently. 31 However, this is less likely an explanation in our study, as the extensive validation process of the multiplex assay did not result in discordant cases for the AGT (M235T) polymorphism.…”

Section: Allele Frequenciescontrasting

confidence: 54%

The contribution of six polymorphisms to cardiovascular risk in a Dutch high-risk primary care population: the HIPPOCRATES project

et al. 2009

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This study was designed to examine the contribution of six polymorphisms to the occurrence of cardiovascular disease (CVD) in a Dutch primary care population with a high prevalence of cardiovascular risk factors. In this cross-sectional case-control study, 232 patients with CVD and 571 event-free controls were studied. Patients were genotyped for the AGTR1 (A1166C), AGT (M235T), ACE (4656rpt), NOS3 (E298D), GNB3 (C825T) and ADD1 (G460W) polymorphisms. Univariate and multivariate odds ratios (ORs) were calculated to assess the relationship between genotypes and CVD. Receiver operating characteristic (ROC) analysis was used to quantify the contribution of the polymorphisms to the prediction of CVD. No differences in either genotype or allele frequencies were found between CVD cases and controls. Multivariate analyses, corrected for multiple testing according to Bonferroni, showed significant protective associations for the T-allele of AGT (OR ¼ 0.55 (0.34-0.84)) and for the T-allele of ADD1 (OR ¼ 0.52 (0.31-0.82)). ROC analysis showed only a very small improvement of CVD risk prediction by adding the six polymorphisms to a model with traditional risk factors. Our data suggest that a major attribution of the six polymorphisms to the cardiovascular risk prediction in a primary care population such as HIPPOCRATES is unlikely.

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Section: Allele Frequenciescontrasting

confidence: 54%

The contribution of six polymorphisms to cardiovascular risk in a Dutch high-risk primary care population: the HIPPOCRATES project

et al. 2009

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“…This is also suggested by Schunkert et al [39] who hypothesized that the difference between males and females is rather a genetic alternation than an effect induced by blood pressure. Another explanation might be the recently indicated misclassification of the AGT (M235 T) genotype [42]. However, in our study the validation process of our multiplex assay did not reveal discordant results for this polymorphism.…”

Section: Males Femalescontrasting

confidence: 52%

The influence of six cardiovascular polymorphisms on a first event of ischemic heart disease is modified by sex and age

Plat

Stoffers²,

Leeuw³

et al. 2009

Coronary Artery Disease

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“…However, the results have been inconsistent. There have been a number of studies that have confirmed a positive association between M235T variants and hypertension (6)(7)(8), but certain studies have confirmed a lack of significant effect of the AGT M235T polymorphism (9,10). This means that different populations may have a different AGT M235T polymorphism.…”

Section: Discussionmentioning

confidence: 99%

Association between the M235T polymorphism of the AGT gene and cytokines in patients with hypertension

Wang

Wan

2011

Experimental and Therapeutic Medicine

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Abstract. The aim of the present study was to explore the association between the M235T polymorphism of the angiotensinogen (AGT) gene and cytokines in patients with essential hypertension (EH). A total of 300 patients with EH and an agematched control group of 150 individuals without EH, secondary hypertension, myocardial infarction and diabetes were enrolled in this study. Polymerase chain reaction combined with restriction fragment length polymorphism (PCR-RFLP) was used to detect variation in the target genotype, and enzyme-linked immunosorbant assay (ELISA) was used to detect the cytokine [interleukin (IL)-1, IL-6 and tumor necrosis factor-α (TNF-α)] concentrations. The AGT gene 235T allele and 235TT genotype frequencies in hypertensive patients were slightly higher than those in the controls. Furthermore, in the hypertensive subjects with the AGT gene 235T allele, the concentrations of IL-1 and TNF-α were significant higher than those in the controls. The results from our study suggest that the higher AGT gene TT genotype and 235T allele frequencies may be risk factors for hypertension. High frequencies of the AGT gene 235T allele and high cytokine concentrations (IL-1 and TNF-α) may promote the transcription and expression of AGT, particularly in hypertensive patients with the 235TT genotype. IntroductionHypertension has been recognized as a significant risk factor for the development of cardiovascular diseases, including coronary heart disease (CHD), myocardial infarction (MI) and stroke, all of which are principal causes of cardiovascular morbidity and mortality in humans. Essential hypertension (EH) is a complex, polygenic disease in which numerous genes control the blood pressure level. Several candidate genes, all selected from the renin-angiotensin system-related genes, have been examined. One of the candidate genes is the gene encoding angiotensinogen (AGT), the precursor of the vasoactive peptide, angiotensin II. The angiotensin I-converting enzyme (ACE) gene has also been associated with EH in the majority of the population. In recent years, the correlation between the molecular variant of the AGT gene, M235T, and EH has started to receive attention (1-6). AGT in the circulation is mainly synthesized in the liver. The plasma AGT level mainly reflects this synthesis. It has been discovered that the expression of the AGT gene in the liver is regulated by certain cytokines. It has been reported that these cytokines [interleukin (IL)-1, IL-6 and tumor necrosis factor-α (TNF-α)] are likely to be the main activation factors in AGT gene expression. They activate the expression of the AGT gene, elevate the concentrations of AGT and ultimately accelerate the development of hypertension. In addition, IL-1 and TNF-α also induce proliferation of the smooth muscle blood vessel cells and arteriosclerosis. Studies on the association between the M235T polymorphism of the AGT gene and cytokines in hypertensive Chinese patients have been rarely reported. The aim of this study was to analyze the M235T polymorphism ...

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Mistyping of Angiotensinogen M235T Alleles

Cited by 9 publications

References 10 publications

The contribution of six polymorphisms to cardiovascular risk in a Dutch high-risk primary care population: the HIPPOCRATES project

The contribution of six polymorphisms to cardiovascular risk in a Dutch high-risk primary care population: the HIPPOCRATES project

The influence of six cardiovascular polymorphisms on a first event of ischemic heart disease is modified by sex and age

Association between the M235T polymorphism of the AGT gene and cytokines in patients with hypertension

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