miRNA profiling of B-cell subsets: specific miRNA profile for germinal center B cells with variation between centroblasts and centrocytes

Tan, Lu; Wang, Miao; Robertus, Jan-Lukas; Schakel, Rikst Nynke; Gibcus, Johan H.; Diepstra, Arjan; Harms, Geert; Peh, Suat‐Cheng; Reijmers, Rogier M.; Pals, Steven T.; Kroesen, Bart-Jan; Kluin, Philip M.; Poppema, Sibrand; Berg, Anke van den

doi:10.1038/labinvest.2009.26

Cited by 99 publications

(91 citation statements)

References 50 publications

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“…These findings confirm the similarity of splenic marginal zone lymphoma with the marginal zone. 12,[24][25][26] As already described in other low-grade lymphomas, 16 the inflammatory cytokines present in the microenvironment may be involved in the onset and progression of disease contributing to the survival of tumor cells, consistently with what is already known about the role of the inflammatory microenvironment in this lymphoma type.…”

Section: Discussionmentioning

confidence: 70%

“…The expression pattern of these miRNAs has been previously reported in the different B-cell stages, so that miR-155, miR-34a and miR-193b were found overexpressed in memory B cells, the converse of what is found in naive B cells. 12 MiR-21 has a higher level of expression in memory cells than in both naive and germinal center cells, 12,13,25 miR-145 is more weakly expressed in memory than in naive B cells, 25 and miR-27b has recently been described as being downregulated in memory B cells relative to both naive and GC B cells. 12,25 In addition, miR-21, miR-193b and miR34a were upregulated in splenic marginal zone lymphoma B cells relative to normal B cells in this series.…”

Section: Discussionmentioning

confidence: 99%

“…12 MiR-21 has a higher level of expression in memory cells than in both naive and germinal center cells, 12,13,25 miR-145 is more weakly expressed in memory than in naive B cells, 25 and miR-27b has recently been described as being downregulated in memory B cells relative to both naive and GC B cells. 12,25 In addition, miR-21, miR-193b and miR34a were upregulated in splenic marginal zone lymphoma B cells relative to normal B cells in this series. Thus, the miRNome of splenic marginal zone lymphoma determined in the present study suggests that this tumor is a neoplastic expansion of memory B cells, similar to what our group has observed for nodal marginal zone lymphoma.…”

Section: Discussionmentioning

confidence: 99%

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Splenic marginal zone lymphoma: comprehensive analysis of gene expression and miRNA profiling

et al. 2013

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Splenic marginal zone lymphoma is a small B-cell neoplasm whose molecular pathogenesis is still essentially unknown and whose differentiation from other small B-cell lymphomas is hampered by the lack of specific markers. We have analyzed the gene expression and miRNA profiles of 31 splenic marginal zone lymphoma cases. For comparison, 7 spleens with reactive lymphoid hyperplasia, 10 spleens infiltrated by chronic lymphocytic leukemia, 12 spleens with follicular lymphoma, 6 spleens infiltrated by mantle cell lymphoma and 15 lymph nodes infiltrated by nodal marginal zone lymphoma were included. The results were validated by qRT-PCR in an independent series including 77 paraffin-embedded splenic marginal zone lymphomas. The splenic marginal zone lymphoma miRNA signature had deregulated expression of 51 miRNAs. The most highly overexpressed miRNAs were miR-155, miR-21, miR-34a, miR-193b and miR-100, while the most repressed miRNAs were miR-377, miR-27b, miR-145, miR-376a and miR-424. MiRNAs located in 14q32-31 were underexpressed in splenic marginal zone lymphoma compared with reactive lymphoid tissues and other B-cell lymphomas. Finally, the gene expression data were integrated with the miRNA profile to identify functional relationships between genes and deregulated miRNAs. Our study reveals miRNAs that are deregulated in splenic marginal zone lymphoma and identifies new candidate diagnostic molecules for splenic marginal zone lymphoma.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Splenic marginal zone lymphoma: comprehensive analysis of gene expression and miRNA profiling

et al. 2013

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“…19 Briefly, 11 digoxigenin-labled locked nucleic acid probes Abbreviations: Ab, antibody; Hb, hemoglobin; HCV, hepatitis C virus; IIL-score, Intergruppo Italiano Linfomi-score; LDH, lactatedehydrogenase; LN, lymph node; MD, missing data; miRNA, microRNA; NA, not applicable; SMZL, splenic marginal zone lymphoma; ULN, upper limit of normal.…”

Section: Global Mirna Expression Analysismentioning

confidence: 99%

Dysregulation of global microRNA expression in splenic marginal zone lymphoma and influence of chronic hepatitis C virus infection

et al. 2012

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The precise molecular pathogenesis of splenic marginal zone lymphoma (SMZL) is still unknown. Clinical and epidemiological data suggest that chronic hepatitis C virus (HCV) infection may have an etiological role in a subset of cases. We performed a large-scale microRNA (miRNA) expression profiling analysis of 381 miRNAs by quantitative reverse transcription PCR (Q-RT-PCR) of 26 microdissected splenic tissue samples (7 HCV þ SMZL; 8 HCV À SMZL and 11 non-neoplastic splenic controls). Single assay Q-RT-PCR and miRNA in situ hybridization (miRNA-ISH) were used to confirm the results in an independent cohort. Unsupervised hierarchical clustering of miRNA expression profiles demonstrated a distinct signature of SMZL compared with the normal splenic marginal zone. Supervised analysis revealed differentially expressed miRNAs, including miRNAs with previously recognized tumor suppressive or oncogenic potential. Five miRNAs were found significantly overexpressed in SMZL, including miR-21, miR-155 and miR-146a, whereas seven miRNAs showed significantly reduced expression, including miR-139, miR-345, miR-125a and miR-126. Furthermore, we identified miR-26b, a miRNA known to have tumor suppressive properties, as significantly downregulated in SMZL arising in HCV-positive patients (P ¼ 0.0016). In conclusion, there is a characteristic dysregulation of miRNA expression in SMZL with a possible implication in its molecular tumorigenesis.

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“…When comparing relative expression levels between pro-B and naive B cells (Monticelli et al, 2005), expression of the following miRNAs was enriched at a specific stage: miR-24, miR-93, miR-101, miR-107, miR-324 in pro-B cells; miR-26, miR-29a, miR-142-3p, miR-142-5p, miR-150 in naive B cells. When comparing relative expression levels between naive, GC and memory B cells (Malumbres et al, 2009;Tan et al, 2009), expression of the following miRNAs was enriched at a specific stage: let7a, and others in memory cells.…”

Section: Microrna Expression In B Cell Differentiation and Maturationmentioning

confidence: 99%

Modulation of Cancer Progression by Tumor Microenvironmental Leukocyte-Expressed microRNAs

Sempere¹,

Conejo-García²

2012

Tumor Microenvironment and Myelomonocytic Cells

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miRNA profiling of B-cell subsets: specific miRNA profile for germinal center B cells with variation between centroblasts and centrocytes

Cited by 99 publications

References 50 publications

Splenic marginal zone lymphoma: comprehensive analysis of gene expression and miRNA profiling

Splenic marginal zone lymphoma: comprehensive analysis of gene expression and miRNA profiling

Dysregulation of global microRNA expression in splenic marginal zone lymphoma and influence of chronic hepatitis C virus infection

Modulation of Cancer Progression by Tumor Microenvironmental Leukocyte-Expressed microRNAs

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