miRNA and mammalian male germ cells

McIver, Skye C; Roman, Shaun D.; Nixon, Brett; McLaughlin, Eileen A.

doi:10.1093/humupd/dmr041

Cited by 137 publications

(105 citation statements)

References 134 publications

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“…Many well-known germ cellspecific proteins were present in this cluster. For example, PIWIL1/MIWI, PIWIL2/MILI, and TDRD1 are germ cell-specific proteins that are involved in piRNA processing and are components of the chromatoid body, which is the RNA storage and processing site (43). SYCP3 and SYCE1 are components of the synaptonemal complex and are essential for the completion of meiosis (44).…”

Section: Resultsmentioning

confidence: 99%

Integrative Proteomic and Transcriptomic Analyses Reveal Multiple Post-transcriptional Regulatory Mechanisms of Mouse Spermatogenesis

Gan

Cai

Lin

et al. 2013

Molecular & Cellular Proteomics

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Mammalian spermatogenesis consists of many cell types and biological processes and serves as an excellent model for studying gene regulation at transcriptional and post-transcriptional levels. Many key proteins, miRNAs, and perhaps piRNAs have been shown to be involved in post-transcriptional regulation of spermatogenesis. However, a systematic method for assessing the relationship between protein and mRNA expression has not been available for studying mechanisms of post-transcriptional regulation. In the present study, we used the iTRAQbased quantitative proteomic approach to identify 2008 proteins in mouse type A spermatogonia, pachytene spermatocytes, round spermatids, and elongative spermatids with high confidence. Of these proteins, 1194 made up four dynamically changing clusters, which reflect the mitotic amplification, meiosis, and post-meiotic development of germ cells. We identified five major regulatory mechanisms termed "transcript only," "transcript degradation," "translation repression," "translation de-repression," and "protein degradation" based on changes in protein level relative to changes in mRNA level at the mitosis/meiosis transition and the meiosis/post-meiotic development transition. We found that post-transcriptional regulatory mechanisms are related to the generation of piRNAs and antisense transcripts. Our results provide a valuable inventory of proteins produced during mouse spermatogenesis and contribute to elucidating the mechanisms of the post-transcriptional regulation of gene expression in mammalian spermatogenesis. Molecular &

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Section: Resultsmentioning

confidence: 99%

Integrative Proteomic and Transcriptomic Analyses Reveal Multiple Post-transcriptional Regulatory Mechanisms of Mouse Spermatogenesis

Gan

Cai

Lin

et al. 2013

Molecular & Cellular Proteomics

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“…Post-transcriptional regulation of gene expression via small non-coding RNA molecules, including miRNA, is recognized as an important mechanism in fine-tuning translational regulation during cell differentiation which is highly active during spermatogenesis (37). Increased miRNA levels coincided with the activation of large-scale gene transcription, peaking at the pachytene spermatocyte stage and the post-meiotic stage during spermatid development (38,39).…”

Section: Small Non-coding Rnas Including Micro-rnas (Mirnas) In Maturmentioning

confidence: 99%

Human spermatozoal RNAs

Hamatani

2012

Fertility and Sterility

117

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“…As in other types of cancer, TGCTs present particular miRNA expression profiles but related with the developmental origin (McIver et al, 2012). The hsa-miR-302 and the hsa-miR-371~373 clusters are overexpressed in seminomas malignant tumors.…”

Section: Small Non-coding Rnas and Tgctsmentioning

confidence: 97%

Endocrine disruptors, gene deregulation and male germ cell tumors

Mazo¹,

Brieño‐Enríquez²,

García-López³

et al. 2013

Int. J. Dev. Biol.

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Endocrine disruptors (EDs) belong to a large group of compounds, usually present as environmental pollutants, which can alter the homeostasis of living organisms by modifying hormonal balance and changing the normal patterns of gene regulation during development and cell differentiation. Hence, the development of male gonads and their functionality may be affected by exposure to specific EDs or their mixtures. The molecular mechanisms of action of these reprotoxicants leading to pathologies of the reproductive system such as testicular cancer, are complex and not well characterized. It is likely, however, that these compounds alter the interaction between the mechanisms of gene regulation and functional gene networks in windows of risk, mainly during embryonic development. Moreover, such changes could be transmitted through generations by epigenetic mechanisms. There are examples of the action of EDs on the expression of mRNAs, small non-coding RNAs and epigenetic marks in the developing testis associated with cellular and molecular alterations found in germ cell tumors. In the present review, we will discuss various aspects of genetic, transcriptomic and epigenetic changes related to testicular development, exposure to EDs and the occurrence of germ cell tumors.

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miRNA and mammalian male germ cells

Cited by 137 publications

References 134 publications

Integrative Proteomic and Transcriptomic Analyses Reveal Multiple Post-transcriptional Regulatory Mechanisms of Mouse Spermatogenesis

Integrative Proteomic and Transcriptomic Analyses Reveal Multiple Post-transcriptional Regulatory Mechanisms of Mouse Spermatogenesis

Human spermatozoal RNAs

Endocrine disruptors, gene deregulation and male germ cell tumors

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