miR390-tasiRNA3-ARF4 pathway is involved in regulating flowering time in woodland strawberry

et al. 2023

Preprint

Background: Juglans mandshurica Maxim. is a monoecious heteromorphic plant. The mechanism of miRNA-mediated sex differentiation and dioecious heterozygosity is unclear. Results：In this study, 310 known and 278 unknown miRNA were identified and 129 differentially expressed miRNA were screened using high-throughput sequencing technology for microRNA sequencing analysis of male and female flower buds of the protogyny and protandry of Juglans mandshurica. 638 miRNA target genes predicted using PSRobots and these target genes are significantly enriched in reproductive, floral development and phytohormone signaling pathways. Therefore, it is hypothesized that miR159c-3p, miR156d-3p, miR172d, miR171b, miR164a, miR167a, miR319a, miR399a, and miR396t et al. may be the key miRNA affecting sex differentiation in Juglans mandshurica, among which miR164a, miR166c, and miR171c-5p may be the key miRNA for heterosexualism differentiation in Juglans mandshurica. 9 randomly selected miRNAs with significant differences were subjected to real-time fluorescence quantitative PCR to verify the accuracy of high-throughput sequencing. Combined with transcriptome sequencing results for co-analysis, a differential miRNA-differential mRNA primary regulatory network was established. Conclusion：It was further confirmed that these miRNA play an important role in the sex differentiation of Juglans mandshurica. This study lays the research foundation for the eventual discovery of the molecular mechanism of sex differentiation and heterosexualism in Juglans mandshurica.

Section: Discussionsupporting

confidence: 90%

Identification and analysis of key miRNA for sex differentiation in hermaphroditic Juglans mandshurica Maxim.

et al. 2023

Preprint

“…For detecting the relative expression of mdm-miR390 and tasiRNA3, SuperReal PreMix (TIANGEN BIOTECH, FP206-02) was used. The previously reported methods were performed [ 31 ]. Apple 5SrRNA was selected as an internal reference.…”

Section: Methodsmentioning

confidence: 99%

“…The miR390-tasiRNA- ARF4 regulatory module contributes to auxin responses in LR developmental processes [ 30 ]. In flowering development, the miR390-tasiRNA3- ARF4 pathway delays woodland strawberry florescence by targeting two genes related to flowering ( FveFUL and FveAP1 ) [ 31 ]. In poplar, the miR390/ TAS3 / ARFs module acts as a key regulator of increased salt tolerance via modulating the auxin signaling [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

MIR390 Is Involved in Regulating Anthracnose Resistance in Apple

Shi

Jiang

et al. 2022

Plants

As an important cash crop in China, apple has a good flavor and is rich in nutrients. Fungal attacks have become a major obstacle in apple cultivation. Colletotrichum gloeosporioides is one of the most devastating fungal pathogens in apple. Thus, discovering resistance genes in response to C. gloeosporioides may aid in designing safer control strategies and facilitate the development of apple resistance breeding. A previous study reported that ‘Hanfu’ autotetraploid apple displayed higher C. gloeosporioides resistance than ‘Hanfu’ apple, and the expression level of mdm-MIR390b was significantly upregulated in autotetraploid plants compared to that in ‘Hanfu’ plants, as demonstrated by digital gene expression (DGE) analysis. It is still unclear, however, whether mdm-MIR390b regulates apple anthracnose resistance. Apple MIR390b was transformed into apple ‘GL-3′ plants to identify the functions of mdm-MIR390b in anthracnose resistance. C. gloeosporioides treatment analysis indicated that the overexpression of mdm-MIR390b reduced fungal damage to apple leaves and fruit. Physiology analysis showed that mdm-MIR390b increased C. gloeosporioides resistance by improving superoxide dismutase (SOD) and peroxidase (POD) activity to alleviate the damage caused by O2− and H2O2. Our results demonstrate that mdm-MIR390b can improve apple plants’ anthracnose resistance.

“…The significance of the differences between the means was analyzed using Duncan’s test in the DPS 7.05 software ( Dong et al., 2022 ). Mean values marked with * and ** indicate significant differences at the 5% and 1% levels, respectively.…”

Section: Methodsmentioning

confidence: 99%

FveARF2 negatively regulates fruit ripening and quality in strawberry

Mao

et al. 2022

Front. Plant Sci.

Self Cite

Auxin response factors (ARFs) are transcription factors that play important roles in plants. ARF2 is a member of the ARF family and participates in many plant growth and developmental processes. However, the role of ARF2 in strawberry fruit quality remains unclear. In this study, FveARF2 was isolated from the woodland strawberry ‘Ruegen’ using reverse transcription-polymerase chain reaction (RT-PCR), which showed that FveARF2 expression levels were higher in the stem than in other organs of the ‘Ruegen’ strawberry. Moreover, FaARF2 was higher in the white fruit stage of cultivated strawberry fruit than in other stage. Subcellular localization analysis showed that FveARF2 is located in the nucleus, while transcriptional activation assays showed that FveARF2 inhibited transcription in yeast. Silencing FveARF2 in cultivated strawberry fruit revealed earlier coloration and higher soluble solid, sugar, and anthocyanin content in the transgenic fruit than in the control fruit, overexpression of FveARF2 in strawberry fruit delayed ripening and lower soluble solid, sugar, and anthocyanin content compared to the control fruit. Gene expression analysis indicated that the transcription levels of the fruit ripening genes FaSUT1, FaOMT, and FaCHS increased in FveARF2-RNAi fruit and decreased in FveARF2-OE fruit, when compared with the control. Furthermore, yeast one-hybrid (Y1H) and GUS activity experiments showed that FveARF2 can directly bind to the AuxRE (TGTCTC) element in the FaSUT1, FaOMT, and FaCHS promoters in vitro and in vivo. Potassium ion supplementation improved the quality of strawberry fruit, while silencing FveARF2 increased potassium ion content in transgenic fruit. The Y1H and GUS activity experiments also confirmed that FveARF2 could directly bind to the promoter of FveKT12, a potassium transporter gene, and inhibited its expression. Taken together, we found that FveARF2 can negatively regulate strawberry fruit ripening and quality, which provides new insight for further study of the molecular mechanism of strawberry fruit ripening.