MiR-940 Inhibited Cell Growth and Migration in Triple-Negative Breast Cancer

Hou, Lingmi; Chen, Maoshan; Yang, Hongwei; Xing, Tianyong; Li, Jingdong; Li, Guangwu; Zhang, Lina; Deng, Shumin; Hu, Jiani; Zhao, Xiaobo; Jiang, Jun

doi:10.12659/msm.897731

Cited by 31 publications

(29 citation statements)

References 30 publications

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“…Moreover, our results also demonstrated that the capacities of proliferation, invasion, migration, and antiapoptosis, in addition to Bcl-2 expression were promoted in the miR-940 mimic, siRNA-SFRP1, and LiCl groups, while the expression of Fas and Bax was significantly reduced when compared with the NC and blank groups; these results could be reversed by miR-940 inhibitor. miR-940 has been reported to inhibit cellular proliferation and migration in triplenegative breast cancer, 13 to enhance progression of cervical cancer by repressing p27 and PTEN 39 and to facilitate invasion and proliferation of pancreatic cancer cells by inhibiting GSK3β and SFRP1. 16 Shih et al 40 confirmed that the SFRP1 expression functioning as an antagonist of the Wnt pathway provides a purported mechanism to suppress the abnormal activation of this pathway.…”

Section: Discussionmentioning

confidence: 99%

“…It is reported that miR‐490 can inhibit the growth of pancreatic tumors by targeting myD88, and can also inhibit the growth of nasopharyngeal carcinoma cells by targeting nestin . However, there are also relevant reports suggesting that microRNA‐940 (miR‐940) can promote the progression of cervical cancer via the inhibition of p27 and PTEN . In addition, even though miR‐940 can inhibit the growth of breast cancer cells by targeting ZNF24, it can promote the growth of gastric cancer cells.…”

Section: Introductionmentioning

confidence: 99%

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Retracted: Inhibition of microRNA‐940 suppresses the migration and invasion of human osteosarcoma cells through the secreted frizzled‐related protein 1–mediated Wnt/β‐catenin signaling pathway

Lin

Zhang

Jiang

et al. 2018

J of Cellular Biochemistry

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Osteosarcoma (OS) is the most common malignant tumor of bone with a high potential for metastasis. This study intends to explore whether microRNA‐940 (miR‐940) affects the development of OS cells and the underlying mechanism. OS and adjacent normal tissues were collected from OS patients; the OS cell line with the highest expression of miR‐940 was selected, which was then subjected to transfection of miR‐940 mimic, miR‐940 inhibitor, siRNA‐secreted frizzled‐related protein 1 (SFRP1) or LiCl (agonists of Wnt/β‐catenin pathway) to identify regulation of miR‐940 to OS cells through SFRP1. The targeting relationship between miR‐940 and SFRP1 was verified using dual‐luciferase reporter gene assay. Reverse‐transcription quantitative polymerase chain reaction and Western blot assay were performed to determine miR‐940, SFRP1, β‐catenin, and cyclinD1 and apoptosis‐related genes Fas, Bax, and Bcl‐2. MTT (3‐(4, 5‐dimethylthiazol‐2‐Yl)‐2, 5‐diphenyltetrazolium bromide) assay, scratch test, transwell assay, and flow cytometry were carried out to detect proliferation, migration, invasion, and apoptosis, respectively. Nude mice models were established to observe the tumor formation. Higher expression of miR‐940, β‐catenin, and cyclinD1 and lower SFRP1 expression were identified in OS tissues. miR‐940 targeted and negatively regulated SFRP1 expression. Furthermore, upregulated miR‐940 expression activated the Wnt/β‐catenin signaling pathway in OS. With the treatment of miR‐940 mimic, LiCL, or siRNA‐SFRP1, OS cells showed promoted proliferation, migration, invasion, tumor formation, and impeded apoptosis (further reflected by elevated Bcl‐2 expression and reduced Fas and Bax expression). The study demonstrates that miR‐940 can promote the proliferation, migration, and invasion but suppress the apoptosis of human OS cells by downregulating SFRP1 through activating Wnt/β‐catenin signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Retracted: Inhibition of microRNA‐940 suppresses the migration and invasion of human osteosarcoma cells through the secreted frizzled‐related protein 1–mediated Wnt/β‐catenin signaling pathway

Lin

Zhang

Jiang

et al. 2018

J of Cellular Biochemistry

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“…It was reported in many studies that miR-940 was highly expressed in normal tissues compared with tumors, and miR-940 was shown to inhibit the migratory and invasive potential of cancer cells by suppressing CXCR2 [59], MIEN1 [58], MyD88 [57], Nestin [56], and ZNF24 [54]. Moreover, the Estrogen-Related Receptor Gamma (ESRRG) was targeted by miR-940, and suppression of ESRRG inhibited hepatocellular carcinoma cell lines growth and induced cell apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Exosomal miR-940 maintains SRC-mediated oncogenic activity in cancer cells: a possible role for exosomal disposal of tumor suppressor miRNAs

Rashed

Kanlikilicer²,

Rodríguez-Aguayo³

et al. 2017

Oncotarget

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Exosomes have emerged as important mediators of diverse biological functions including tumor suppression, tumor progression, invasion, immune escape and cell-to-cell communication, through the release of molecules such as mRNAs, miRNAs, and proteins. Here, we identified differentially expressed exosomal miRNAs between normal epithelial ovarian cell line and both resistant and sensitive ovarian cancer (OC) cell lines. We found miR-940 as abundant in exosomes from SKOV3-IP1, HeyA8, and HeyA8-MDR cells. The high expression of miR-940 is associated with better survival in patients with ovarian serous cystadenocarcinoma. Ectopic expression of miR-940 inhibited proliferation, colony formation, invasion, and migration and triggered G0/G1 cell cycle arrest and apoptosis in OC cells. Overexpression of miR-940 also inhibited tumor cell growth in vivo. We showed that proto-oncogene tyrosine-protein kinase (SRC) is directly targeted by miR-940 and that miR-940 inhibited SRC expression at mRNA and protein levels. Following this inhibition, the expression of proteins downstream of SRC, such as FAK, paxillin and Akt was also reduced. Collectively, our results suggest that OC cells secrete the tumor-suppressive miR-940 into the extracellular environment via exosomes, to maintain their invasiveness and tumorigenic phenotype.

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“…MicroRNAs (miRNAs) comprise a series of endogenous, short single-stranded non-coding RNAs (19)(20)(21)(22)(23)(24)(25) molecules) that primarily serve as gene regulators (8). These molecules negatively regulate gene expression by directly binding to partial complimentary sites in the 3'-untranslated regions (3'-UTRs) of their target genes, resulting in mRNA degradation or inhibition of translation (9).…”

Section: Introductionmentioning

confidence: 99%

“…miR-940 has been well studied in the pathogenesis of a number of types of human cancer (18)(19)(20)(21)(22); however, the expression pattern, roles and molecular mechanisms of the regulatory actions of miR-940 in glioma remain unknown. The present study aimed to further understand the roles of miR-940 by…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑940 promotes cell proliferation and invasion of glioma by directly targeting Kruppel‑like factor�9

et al. 2018

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has been extensively studied in the pathogenesis of numerous types of human cancer; however, the expression pattern, roles and molecular mechanisms underlying the regulatory actions of miR-940 in glioma remain unknown. The present study aimed to further investigate miR-940 by studying its expression, roles and mechanisms of action in glioma. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect miR-940 expression in glioma tissues and cell lines. The regulatory effects of miR-940 in glioma cell proliferation and invasion were determined using MTT and cell invasion assays. Bioinformatics analyses was performed to identify the potential target of miR-940, which was further confirmed by luciferase reporter assay, RT-qPCR and western blot analysis. In the present study, significantly increased miR-940 expression levels were observed in glioma tissues and cell lines compared with normal brain tissues and normal human astrocytes, respectively. Decreased miR-940 expression levels attenuated glioma cell proliferation and invasion in vitro. Kruppel-like factor 9 (KLF9) was predicted as a potential target of miR-940. Further assays demonstrated that miR-940 negatively regulated KLF9 expression in glioma cells by directly targeting the 3'-untranslated regions of KLF9. Additionally, KLF9 expression was downregulated in glioma tissues and was inversely correlated with miR-940. Furthermore, KLF9 knockdown was able to rescue the effects of miR-940 on glioma cell proliferation and invasion. The results of the present study suggest that miR-940 may function as an oncogene in glioma by targeting KLF9 and may be a considered a therapeutic target for the treatment of gliomas.

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MiR-940 Inhibited Cell Growth and Migration in Triple-Negative Breast Cancer

Cited by 31 publications

References 30 publications

Retracted: Inhibition of microRNA‐940 suppresses the migration and invasion of human osteosarcoma cells through the secreted frizzled‐related protein 1–mediated Wnt/β‐catenin signaling pathway

Retracted: Inhibition of microRNA‐940 suppresses the migration and invasion of human osteosarcoma cells through the secreted frizzled‐related protein 1–mediated Wnt/β‐catenin signaling pathway

Exosomal miR-940 maintains SRC-mediated oncogenic activity in cancer cells: a possible role for exosomal disposal of tumor suppressor miRNAs

MicroRNA‑940 promotes cell proliferation and invasion of glioma by directly targeting Kruppel‑like factor�9

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