miR‐330‐5p inhibits proliferation and migration of keratinocytes by targeting Pdia3 expression

Abstract

The microRNAs (miRNAs) are a class of small non-coding RNA molecules that play important roles in cellular processes by regulating gene expression at the post-transcriptional level. In skin biology, several miRNAs have been shown to be associated with differentiation, migration and apoptosis of keratinocytes, and regulation of the hair cycle. Although the biological role of miR-330-5p has been reported in several cancers and cells, the function and molecular mechanism of miR-330-5p in skin keratinocytes have not been identified. In this study, we found that miR-330-5p inhibited the proliferation and migration of mouse keratinocytes. Among the candidate target genes of miR-330-5p searched using microarray analysis, we found that the expression of Pdia3 was directly regulated by miR-330-5p in the mouse keratinocyte. Moreover, inhibition of Pdia3 expression caused decreased proliferation and migration ability of mouse keratinocytes. Additionally, expressions of miR-330-5p and Pdia3 displayed an inverse correlation with respect to the hair cycle stage. These results indicated that regulation of Pdia3 expression by miR-330-5p is important in maintaining the hair cycle through regulation of the proliferation and migration capability of keratinocytes.

Long non-coding RNA EWSAT1 promotes human nasopharyngeal carcinoma cell growth in vitro by targeting miR-326/-330-5p

“…MiR-326/330-5p can regulate numerous of target genes. Recent study indicated that miR-330-5p inhibits proliferation and migration of keratinocytes by targeting Pdia3 expression [52], and regulates tyrosinase and PDIA3 expression and suppresses cell proliferation and invasion in cutaneous malignant melanoma [48]. MiR-326 reverses chemoresistance in human lung adeno-carcinoma cells by targeting specificity protein 1 [53], and regulates cell proliferation and migration in lung cancer by targeting phox2a and is regulated by HOTAIR [54].…”

Myalgic encephalomyelitis or chronic fatigue syndrome: how could the illness develop?

“…In addition, miR-126 regulates cytosolic TLR signalling and modulates the duration and intensity of such signalling in DCs (Rogers and Herzog 2014). Finally, miR-330 also regulates the activity of the PI3K/Akt signalling pathway and also exerts an inhibitory effect on T cell proliferation and NK cell activation (Grigoryev et al 2011; Kim et al 2015a; Petty et al 2016). …”

Differential roles of exogenous protein disulfide isomerase A3 on proliferating cell and neuroblast numbers in the normal and ischemic gerbils

“…PDIA3 also contributes to neurite outgrowth by modulating ER calcium release and cytoplasmic dynamics (LeBlanc & Nemere, 2014). Overexpression of PDIA3 in mice facilitates the regeneration of sciatic nerve after mechanical injury (Castillo et al, 2015), while PDIA3 inhibition in mouse keratinocytes decreases their ability of proliferation and migration (Kim, Yoo, Choi, & Yoon, 2015). However, in central nervous system, PDIA3 would not cross the blood-brain barrier to act directly on hippocampus.…”