miR-31 affects colorectal cancer cells by inhibiting autophagy in cancer-associated fibroblasts

Yang, Xiaodong; Xu, Xiaohui; Zhu, Jinming; Zhou, Gang; Wu, Yong; Wu, Yongyou; Zhao, Kui; Cao, Jianping; Zhu, Hong; Li, Ming; Ye, Zhenyu; Peng, Wei

doi:10.18632/oncotarget.12873

Cited by 71 publications

(35 citation statements)

References 29 publications

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“…Consistent with the report above, our results in this study also discovered that miR-31 was high-expressed in CRC tissues. What's more, miR-31 is proved to be correlated to the metastasis of CRC and can regulate autophagy, proliferation, and chemoresistance of CRC cells (16,29,30). Similarly, in this study, the results con rmed that miR-31 overexpression not only promoted the migration, invasion, and suppressed the apoptosis of CRC cells but also counteracted the effect of circ_0026344.…”

Section: Discussionsupporting

confidence: 74%

Circ_0026344 overexpression inhibits the migration, invasion, and enhances apoptosis of colorectal cancer cells by sponging miR-31

Jin

Zhang

et al. 2020

Preprint

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Background: Circ_0026344 was reported to be associated with the metastasis of colorectal cancer (CRC). This study aimed to investigate the expression of circ_0026344 in CRC and the effect mechanisms of circ_0026344 on CRC.Methods: The expressions of circ_0026344 and miR-31 in clinical CRC tissues or CRC cell lines were analyzed by qPCR. The target of circ_0026344 was predicted and verified by CircInteractome and dual-luciferase reporter assays. The correlation between circ_0026344 and miR-31 expression was analyzed using Pearson analysis. After the CRC cells were overexpressed circ_0026344 or miR-31 or silenced circ_0026344, the viability, apoptosis, migration, and invasion of CRC cells were evaluated by CCK-8, flow cytometry, wound healing, and transwell. Also, the expressions of miR-31, Bcl-2, Bax, E-cadherin, and N-cadherin in the cells were detected by qPCR or Western blot. Results: Circ_0026344 was low-expressed in CRC tissues and cell lines. Circ_0026344 sponged miR-31 which was high-expressed in CRC tissues. The expression of circ_0026344 was negatively correlated to the expression of miR-31. The miR-31 expression could be down-regulated by circ_0026344 overexpression. Circ_0026344 overexpression inhibited the cell viability, migration, and invasion; and enhanced the apoptosis of CRC cells. Circ_0026344 overexpression decreased the expressions of Bcl-2 and N-cadherin and increased the expressions of Bax and E-cadherin in CRC cells. Circ_0026344 silencing and miR-31 overexpression had an opposite effect on CRC cells as circ_0026344 overexpression. Furthermore, miR-31 overexpression counteracted the effect of circ_0026344 overexpression.Conclusion: Circ_0026344 overexpression inhibited the migration, invasion, and enhanced apoptosis of CRC cells by sponging miR-31.

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Section: Discussionsupporting

confidence: 74%

Circ_0026344 overexpression inhibits the migration, invasion, and enhances apoptosis of colorectal cancer cells by sponging miR-31

Jin

Zhang

et al. 2020

Preprint

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“…29 Moreover, Yang et al displayed miR-31 regulated colorectal cancer via autophagy. 30 At presently, there was wealth of researches on miR-31 in cancer. Studies had proved that miR-31 was highly expressed in colorectal cancer, lung cancer, and other cancers, but it was low in bladder cancer, gastric cancer, and other cancers.…”

Section: Discussionmentioning

confidence: 99%

Retracted: MicroRNA‐31 weakens cisplatin resistance of medulloblastoma cells via NF‐κB and PI3K/AKT pathways

Cao

Ding³

2020

BioFactors

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Background: Medulloblastoma (MB) is a malignant intracranial tumor. Cisplatin is a broad-spectrum antitumor drug. It is important to study the cisplatin resistance of MB cells for the treatment of MB. In this article, we preliminarily studied the cisplatin resistance of microRNA (miR)-31 and the possible mechanism in DAOY and UW228 cells, laying a theoretical foundation for clinical treatment of MB. Methods: Following anti-miR-31 and pre-miR-31 transfections, cell viability, BrdU, CyclinD1, and apoptosis levels of DAOY and UW228 cell were detected by CCK8, BrdU, and western blot. Meanwhile, migration, invasion, and western blot assay were respectively used to detect the functions of miR-31 migration and invasion. miR-31 levels were changed by cell transfection and detected by RT-qPCR. Furthermore, the related-proteins of pathways were also detected by western blot. Results: Anti-miR-31 increased DAOY and UW228 cells viability, BrdU + numbers, and expression of CyclinD1. The migration/invasion rate and expression levels of MMP-9 and vimentin after anti-miR-31 transfection were increased. Furthermore, anti-miR-31 enhanced cells' cisplatin resistance and triggered PI3K/AKT and NF-κB pathways. Pre-miR-31 played opposite roles and promoted the apoptosis. Conclusion: miR-31 regulated cell growth, migration, invasion and cisplatin resistance of MB cells via PI3K/AKT and NF-κB pathways.

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“…However, in CRC cells, LOC554202 has a tumor suppressor role in the activation of specific caspase cleavage cascades, and low expression of LOC554202 was associated with advanced pathologic stage and increased tumor size (32). miR-31 is a widely investigated miRNA and studies have reported controversial conclusions regarding its role in CRC cells (52)(53)(54)(55)(56). These studies suggest that a high expression of this miRNA in cancer does not always result in a decreased tumor growth, including that noted in cases of the oncosuppressors p53 and phosphatase and tensin homolog.…”

Section: Os ---------------------------------------------------------mentioning

confidence: 99%

High expression of microRNA‑31 and its host gene LOC554202 predict favorable outcomes in patients with colorectal cancer treated with oxaliplatin

Xin

et al. 2018

Oncol Rep

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The expression levels of microRNA‑31 (miR‑31) and LOC554202 have been previously investigated in colorectal cancer (CRC) and their oncogenic and/or tumor suppressive roles have been described. The aim of the present study was to examine the role of miR‑31 and its host gene LOC554202 in the prognosis of patients with CRC. Patients with CRC treated with oxaliplatin‑based chemotherapy between June 2005 and March 2010 were recruited to the First Affiliated Hospital of China Medical University. Tumor and adjacent mucosal tissues were collected. The detection of miR‑31 and/or LOC554202 was performed with probe hybridization targeting. Correlation analysis was performed among the expression levels of miR‑31, LOC554202, and their association with clinicopathological parameters and/or survival rates. miR‑31 and LOC554202 were expressed at high levels in CRC (P<0.01) compared with adjacent intestinal mucosa. A linear correlation was noted for the two markers in CRC tissues (P<0.01). The expression of miR‑31 was significantly higher in adenocarcinoma than in the adjacent intestinal mucosa (P<0.01), whereas the expression of LOC554202 was significantly higher in the adenocarcinoma and the rectal cancer tissue regions (P<0.01). The high expression levels of miR‑31 and LOC554202 were associated with high disease‑free survival (DFS) and overall survival (OS) rates (P<0.05). Associations between the increase in DFS and OS and the elevated expression levels of miR‑31 and LOC554202 were present in patients with colon cancer but not in patients with rectal cancer (P<0.05). These data indicated that miR‑31 and LOC554202 may be potential markers for evaluation of the prognosis of patients treated with oxaliplatin‑based chemotherapy.

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miR-31 affects colorectal cancer cells by inhibiting autophagy in cancer-associated fibroblasts

Cited by 71 publications

References 29 publications

Circ_0026344 overexpression inhibits the migration, invasion, and enhances apoptosis of colorectal cancer cells by sponging miR-31

Circ_0026344 overexpression inhibits the migration, invasion, and enhances apoptosis of colorectal cancer cells by sponging miR-31

Retracted: MicroRNA‐31 weakens cisplatin resistance of medulloblastoma cells via NF‐κB and PI3K/AKT pathways

High expression of microRNA‑31 and its host gene LOC554202 predict favorable outcomes in patients with colorectal cancer treated with oxaliplatin

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