miR-28-3p Is a Cellular Restriction Factor That Inhibits Human T Cell Leukemia Virus, Type 1 (HTLV-1) Replication and Virus Infection

Bai, Xue; Nicot, Christophe

doi:10.1074/jbc.m114.626325

Cited by 53 publications

(58 citation statements)

References 52 publications

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“…Specifically, we found that levels of miR-28-3p were positively correlated with GRACE risk score and that miR-3135b was inversely correlated with GRACE risk score. MiR-28-3p, found in a variety of tissues, has been found to be associated with pulmonary embolism [39], colorectal cancer [40], and human T cell leukemia virus [41] but this is the first report to our knowledge showing a correlation of miR-28-3p with ACS risk. Further studies are needed to examine the role of miR-28-3p as an important biomarker in ACS and further mechanistic work to elucidate a potential therapeutic role for downregulating miR-28-3p levels in ACS.…”

Section: Discussionmentioning

confidence: 94%

Whole blood sequencing reveals circulating microRNA associations with high-risk traits in non-ST-segment elevation acute coronary syndrome

et al. 2017

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Section: Discussionmentioning

confidence: 94%

Whole blood sequencing reveals circulating microRNA associations with high-risk traits in non-ST-segment elevation acute coronary syndrome

et al. 2017

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“…Also, the location of a miRNA binding site in the 3’ UTR within 15 nucleotides from the stop codon can increase efficacy and repression of the target RNA [38]. However, not all naturally encoded viral miRNA interaction domains encode more than one miRNA binding site [17,18,41,42], and if there is more than one miRNA binding site, the sites may not be ideally located based on the aforementioned guidelines for cooperativity.…”

Section: Discussionmentioning

confidence: 99%

Cooperativity between the 3’ untranslated region microRNA binding sites is critical for the virulence of eastern equine encephalitis virus

Trobaugh

Sun

Bhalla

et al. 2019

Preprint

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28Eastern equine encephalitis virus (EEEV), a mosquito-borne RNA virus, is one of the 29 most acutely virulent viruses endemic to the Americas, causing between 30% and 70% 30 mortality in symptomatic human cases. A major factor in the virulence of EEEV is the 31 presence of four binding sites for the hematopoietic cell-specific microRNA, miR-142-32 3p, in the 3' untranslated region (3' UTR) of the virus. Three of the sites are "canonical" 33 with all 8 seed sequence residues complimentary to miR-142-3p while one is "non-34 canonical" and has a seed sequence mismatch. Interaction of the EEEV genome with 35 miR-142-3p limits virus replication in myeloid cells and suppresses the systemic innate 36 immune response, greatly exacerbating EEEV neurovirulence. The presence of the 37 miRNA binding sequences is also required for efficient EEEV replication in mosquitoes 38 and, therefore, essential for transmission of the virus. In the current studies, we have 39 examined the role of each binding site by point mutagenesis of seed sequences in all 40 combinations of sites followed by infection of mammalian myeloid cells, mosquito cells 41 and mice. The resulting data indicate that both canonical and non-canonical sites 42 contribute to cell infection and animal virulence, however, surprisingly, all sites are 43 rapidly deleted from EEEV genomes shortly after infection of myeloid cells or mice. 44Finally, we show that the virulence of a related encephalitis virus, western equine 45 encephalitis virus, is also dependent upon miR-142-3p binding sites. 46 47 Author Summary 48Eastern equine encephalitis virus (EEEV) is one of the most acutely virulent mosquito-49 borne viruses in the Americas. A major determinant of EEEV virulence is a mammalian 50 microRNA (miRNA) that is primarily expressed in myeloid cells, miR-142-3p. Like 51 miRNA suppression of host mRNA, miR-142-3p binds to the 3' untranslated region 52 (UTR) of the EEEV genome only in myeloid cells suppressing virus replication and the 53 induction of the innate immune response. In this study, we used point mutations in all 54 four miR-142-3p binding sites in the EEEV 3' UTR to understand the mechanism behind 55 this miRNA suppression. We observed that decreasing the number of miR-142-3p 56 binding sites leads to virus escape and ultimately attenuation in vivo. Furthermore, 57 another virus, western equine encephalitis virus, also encodes miR-142-3p binding sites 58 that contribute to virulence in vivo. These results provide insight into the mechanism of 59 how cell-specific miRNAs can mediate suppression of virus replication. 60 61 Eastern equine encephalitis virus (EEEV) is a mosquito-borne alphavirus that 63 causes severe manifestations of encephalitis in humans resulting in high mortality rates 64 and long-term neurological sequelae in symptomatic cases [1] and is one of, if not the 65 most acutely virulent virus circulating in North America. Even though both EEEV and 66 the closely related Venezuelan equine encephalitis virus (VEEV) cause encephalitic 67 disease, th...

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“…In vitro studies show miRNA target influenza; vesicular stomatitis virus, human T-cell leukemia virus 1; human papillomavirus; and enterovirus 71, and they inhibit viral replication ( Fig. 7.3), which facilitates the clearance or potentiating viral latency of the pathogen (Bai and Nicot, 2015;Hen et al, 2014;Zheng et al, 2013). MiRNAs were also essential to show malaria transcripts translocation into the parasite (150).…”

Section: Micrornas In Emerging Diseasementioning

confidence: 99%

“…AA, Poly A tail; m7G, 7-methylguanosine cap; miRNAs, microRNA; ORF, open reading frame; RNA, ribonucleic acid. (Bai and Nicot, 2015). Although a few scientists agree on the direct interaction between miRNAs and viral transcripts, Bogerd et al (2014) argue that cellular miRNAs do not target viruses as global downregulation of host cell miRNAs (via DICER knockout) does not lead to the enhancement of 11 viruses in human embryonic kidney cell line 293T.…”

Section: Micrornas In Emerging Diseasementioning

confidence: 99%

Scientific Advances in the Diagnosis of Emerging and Reemerging Viral Human Pathogens

Hammou

Benhassou

Bessi

et al. 2020

Emerging and Reemerging Viral Pathogens

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miR-28-3p Is a Cellular Restriction Factor That Inhibits Human T Cell Leukemia Virus, Type 1 (HTLV-1) Replication and Virus Infection

Cited by 53 publications

References 52 publications

Whole blood sequencing reveals circulating microRNA associations with high-risk traits in non-ST-segment elevation acute coronary syndrome

Whole blood sequencing reveals circulating microRNA associations with high-risk traits in non-ST-segment elevation acute coronary syndrome

Cooperativity between the 3’ untranslated region microRNA binding sites is critical for the virulence of eastern equine encephalitis virus

Scientific Advances in the Diagnosis of Emerging and Reemerging Viral Human Pathogens

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