miR-196a targeting LRIG3 promotes the proliferation and migration of cervical cancer cells

Qiu, Yunfen; Han, Qing; Lü, Hong; Shi, C

doi:10.14715/cmb/2020.66.7.27

Cited by 4 publications

(3 citation statements)

References 17 publications

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“…miR-196a plays an important regulatory role in organisms and has been found to be upregulated in a variety of malignant tumors. miR-196a affects the proliferation, invasion, and apoptosis of tumor cells and is expected to become a new molecular marker or target for tumor diagnosis, treatment, and prognosis, indicating its potential in clinical applications [ 38 , 39 , 40 , 41 ]. Several studies have also reported that miR-196b is highly expressed in multiple malignant tumors and has the characteristics of an oncogene to promote tumorigenesis [ 42 , 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Roles of miR-196a and miR-196b in Zebrafish Motor Function

et al. 2023

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Background: The exertion of motor function depends on various tissues, such as bones and muscles. miR-196 has been widely studied in cancer and other fields, but its effect on bone and skeletal muscle is rarely reported. In order to explore the role of miR-196 family in bone and skeletal muscle, we used the previously successfully constructed miR-196a-1 and miR-196b gene knockout zebrafish animal models for research. Methods: The behavioral trajectories of zebrafish from 4 days post-fertilization (dpf) to 7 dpf were detected to analyze the effect of miR-196a-1 and miR-196b on motor ability. Hematoxylin-eosin (HE) staining and transmission electron microscopy (TEM) were used to detect the dorsal muscle tissue of zebrafish. The bone tissue of zebrafish was detected by microcomputed tomography (micro-CT). Real-time PCR was used to detect the expression levels of related genes, including vcp, dpm1, acta1b, mylpfb, col1a1a, bmp8a, gdf6a, and fgfr3. Results: The behavioral test showed that the total behavioral trajectory, movement time, and movement speed of zebrafish larvae were decreased in the miR-196a-1 and miR-196b gene knockout lines. Muscle tissue analysis showed that the structure of muscle fibers in the zebrafish lacking miR-196a-1 and miR-196b was abnormal and was characterized by vacuolar degeneration of muscle fibers, intranuclear migration, melanin deposition, and inflammatory cell infiltration. Bone CT examination revealed decreased bone mineral density and trabecular bone number. The real-time PCR results showed that the expression levels of vcp, dpm1, gdf6a, fgfr3, and col1a1a were decreased in the miR-196b gene knockout group. The expression levels of dpm1, acta1b, mylpfb, gdf6a, and col1a1a were decreased, and the expression level of fgfr3 was increased in the miR-196b gene knockout group compared with the wild-type group. Conclusions: miR-196a-1 and miR-196b play an important role in muscle fiber structure, bone mineral density, and bone trabecular quantity by affecting the expression of vcp, dpm1, acta1b, mylpfb, gdf6a, fgfr3, and col1a1a and then affect the function of the motor system

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Section: Discussionmentioning

confidence: 99%

Roles of miR-196a and miR-196b in Zebrafish Motor Function

et al. 2023

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“…Notably, Lrig3 expression is directly modulated by miRNA-196a, a microRNA that is upregulated in gastric, colon, breast and pancreatic cancer and promotes tumorigenesis. In cervical cancer cells, miRNA-196a targets Lrig3 (19) and improves cell viability. Lrig3 is also regulated at the post-transcriptional level, as there are reports of a circular form of Lrig3 (circRNA Lrig3 ) that is highly expressed in human hepatocellular carcinoma (HCC).…”

Section: Discussionmentioning

confidence: 99%

Lrig3 restricts the size of the colon stem cell compartment

Stevenson

Sayegh

Pedicino

et al. 2022

Preprint

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The cellular census of the colonic crypt is tightly regulated, yet the molecular mechanisms that regulate this census are not fully understood. Lrig3, a transmembrane protein, is expressed in colonic crypt epithelial cells, including the stem, progenitor, and differentiated cell types. Mice missing Lrig3 have a disruption in their cellular census: using a novel Lrig3-/- mouse we demonstrate that Lrig3-/- mice have more cells per crypt, a greater mucosal area, and longer colons compared to wildtype mice, suggesting the expression of Lrig3 is required for both the total number of epithelial cells in the mouse colon, as well as colon length. In addition, we show Lrig3-/- mice have significantly more stem, progenitor, and deep crypt secretory cells, yet harbor a normal complement of enteroendocrine, Tuft, and absorptive cells. Lrig3-/- mice also have a concomitant decrease in phosphorylated Extracellular signal-related kinases, indicating the loss of Lrig3 leads to an expansion of the colonic stem cell compartment, in an Erk-dependent manner. Our study describes the expression of Lrig3 within the colon, defines perturbations in mice lacking Lrig3, and supports a role for Lrig3 in the establishment of both colonic crypt structure and cellular census, defined as the epithelial cell type and number in colon crypts.

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“…CC is the most common in Sub-Saharan Africa, accounting for more than 90% of all cases and 70% of cervical cancers occur in developing countries [6]. HIV (Human Immunodeficiency Virus) affected women must be aware of this cancer because these patients are significantly vulnerable to getting infected by cancer [7].…”

Section: Introductionmentioning

confidence: 99%

Early-Stage Cervical Cancerous Cell Detection from Cervix Images Using YOLOv5

Ontor¹,

Ali²,

Ahmed³

et al. 2023

Computers, Materials &Amp; Continua

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Cervical Cancer (CC) is a rapidly growing disease among women throughout the world, especially in developed and developing countries. For this many women have died. Fortunately, it is curable if it can be diagnosed and detected at an early stage and taken proper treatment. But the high cost, awareness, highly equipped diagnosis environment, and availability of screening tests is a major barrier to participating in screening or clinical test diagnoses to detect CC at an early stage. To solve this issue, the study focuses on building a deep learning-based automated system to diagnose CC in the early stage using cervix cell images. The system is designed using the YOLOv5 (You Only Look Once Version 5) model, which is a deep learning method. To build the model, cervical cancer pap-smear test image datasets were collected from an open-source repository and these were labeled and preprocessed. Then the YOLOv5 models were applied to the labeled dataset to train the model. Four versions of the YOLOv5 model were applied in this study to find the best fit model for building the automated system to diagnose CC at an early stage. All of the model's variations performed admirably. The model can effectively detect cervical cancerous cell, according to the findings of the experiments. In the medical field, our study will be quite useful. It can be a good option for radiologists and help them make the best selections possible.

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miR-196a targeting LRIG3 promotes the proliferation and migration of cervical cancer cells

Cited by 4 publications

References 17 publications

Roles of miR-196a and miR-196b in Zebrafish Motor Function

Roles of miR-196a and miR-196b in Zebrafish Motor Function

Lrig3 restricts the size of the colon stem cell compartment

Early-Stage Cervical Cancerous Cell Detection from Cervix Images Using YOLOv5

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