miR-194 is a marker of hepatic epithelial cells and suppresses metastasis of liver cancer cells in mice

Meng, Zhipeng; Fu, Xianghui; Chen, Xiaosong; Zeng, Samuel; Tian, Yan; Jove, Richard; Xu, Rongzhen; Huang, Wendong

doi:10.1002/hep.23915

Cited by 188 publications

(180 citation statements)

References 51 publications

(53 reference statements)

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“…In addition, in cancer of the gut (37,38) and kidney (39), miR-194 served a similar inhibitory role. By contrast, it was suggested that miR-194 served a role in the promotion and progression of breast cancer (9) and pancreatic ductal adenocarcinoma (40).…”

Section: Discussionmentioning

confidence: 94%

“…By contrast, it was suggested that miR-194 served a role in the promotion and progression of breast cancer (9) and pancreatic ductal adenocarcinoma (40). These studies indicated that miR-194 had various target genes (31)(32)(33)(34)(35)(36)(37)(38)(39)(40). Previous studies have demonstrated that miR-194 inhibited cell migration and invasion, which may have contributed to the anti-tumor activity of trastuzumab on HER2-overexpressing breast cancer cells (41,42).…”

Section: Discussionmentioning

confidence: 99%

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Promotional effect of microRNA-194 on breast cancer cells via targeting F-box/WD repeat-containing protein 7

et al. 2018

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Abstract. Breast cancer is the most common type of malignant cancer in females. An increasing number of studies have revealed that microRNAs (miR), which belong to a class of small non-coding RNAs, serve an important role in a number of human cancer subtypes. In the present study, the role of miR-194 in breast cancer cells and its underlying mechanisms were investigated. The results demonstrated that the serum levels of miR-194 were significantly higher in patients of the poorly differentiated and well-differentiated groups, compared with in healthy adults. Additionally, the serum level of miR-194 was significantly higher in the poorly differentiated group compared with in the well-differentiated group. In order to further investigate the role of miR-194 in breast cancer cells, the present study transfected two breast cancer cell lines, MCF-7 and MDA-MB-231, with an empty vector (control), miR-194 (overexpression), antagomiR-194 (inhibitor, functional knock down) or antagomiR-194 and miR-194. An MTT assay was performed in order to detect the proliferation of breast cancer cells in the various groups. The results revealed that the overexpression of miR-194 significantly accelerated cell proliferation, whereas the inhibition of miR-194 significantly decelerated the proliferation of MCF-7 and MDA-MB-231 cells. Furthermore, the expression levels of cyclin D and cyclin E were significantly upregulated in miR-194 overexpressing cells, and the expression levels of cyclin D and cyclin E were significantly downregulated in miR-194 inhibited cells, as compared with in control cells. No significant change was observed in the level of proliferation of cells co-transfected with miR-194 and antagomiR-194, compared with in the control cells. According to the hypothesis suggesting possible target genes of miR-194, the present study proposed that F-box/WD repeat-containing protein 7 (Fbxw-7) may be a direct target of miR-194, which was confirmed by a luciferase reporter assay. The present study suggested that miR-194 expression promoted the proliferation of breast cancer cells by targeting Fbxw-7, and may serve as a biomarker and a novel target for breast cancer therapy.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Promotional effect of microRNA-194 on breast cancer cells via targeting F-box/WD repeat-containing protein 7

et al. 2018

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“…Moreover, Jin et al (23) overexpression of miR-194 in highly metastatic pancreatic ductal adenocarcinoma, and overexpression of miR-215 in hepatocellular carcinoma by microarray and real-time PCR methods (7-9). However, miR-194 in mouse hepatocellular carcinoma and cancer cell lines was downregulated by the real-time PCR method (24). Therefore, the altered miRNA expression levels may cause tissue-specific differences.…”

Section: Discussionmentioning

confidence: 99%

microRNA-192, -194 and -215 are frequently downregulated in colorectal cancer

Chiang

Song

Wang

et al. 2011

Experimental and Therapeutic Medicine

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Abstract. microRNAs (miRNAs) are small, non-coding RNAs of endogenous origin. They have been increasingly shown to have aberrant expression in a number of tumor types. miR-192, -194 and -215 have not been comprehensively investigated using a large number of cases in colorectal cancer (CRC). We extracted total RNA from 107 CRC tissues and three CRC cell lines. Following polyadenylation and reverse transcription, the expression levels of miR-192, -194 and -215 were determined for evaluation of the association between expression levels and clinicopathological characteristics by a quantitative real-time polymerase chain reaction (real-time PCR) method. Finally, we studied the impact of miR-194 on cell proliferation in HCT-116 cells by MTT assay. miR-192, -194 and -215 were significantly downregulated in CRC tissues (all p<0.001, paired t-test) and cancer cell lines (all p<0.05) compared to non-tumor counterparts. Moreover, the expression levels of miR-192, -194 and -215 were demonstrated to be associated with increased tumor sizes (p=0.027, p=0.018, and p=0.027, respectively; Mann-Whitney U test). Also, there were marked correlations among these miRNAs in CRC tissues (all p<0.001, Pearson's regression analysis). Furthermore, we found that the overexpression of miR-194 could significantly inhibit cell proliferation in may be important biological markers as tumor suppressors in the carcinogenesis of CRC.

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“…The livers were prepared as either paraffin sections or frozen section as described previously. 13,14 The sections were stained with hematotxylin and eosin (H&E) or in situ cell death kit (Roche Applied Science, Indianapolis, IN, USA). To quantify the number of cell deaths, six fields were randomly chosen from each liver section.…”

Section: Stainingmentioning

confidence: 99%

The nuclear receptor CAR modulates alcohol-induced liver injury

Chen

Meng

Wang

et al. 2011

Laboratory Investigation

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The constitutive androstane receptor (CAR) is a member of the nuclear receptor superfamily and a sensor and detoxifier of both xenobiotics and endobiotics. Recent studies also show that CAR participates in metabolism of glucose and lipid, and has an important role in fatty liver disease and diabetes. In this study, we investigate the roles of CAR in chronic and acute alcohol-induced liver injuries. The results showed that absence of CAR in rodents led to significantly increased susceptibility to chronic alcohol-induced liver injury, which was accompanied with elevated hepatocyte apoptosis and fat accumulation. However, pre-activation of CAR by a CAR agonist, TCPOBOP, strongly enhanced the hepatic toxicity by both chronic and acute alcohol infusion in wild-type, but not in CAR À/À mice. Gene expression analyses indicated that CAR pre-activation and alcohol infusion synergistically decreased the expression of enzymes that metabolize the alcohol in liver. These results support a role of CAR in modulating alcoholic liver injury and imply a risk of synergistic liver toxicity induced by alcohol and CAR activation.

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miR-194 is a marker of hepatic epithelial cells and suppresses metastasis of liver cancer cells in mice

Cited by 188 publications

References 51 publications

Promotional effect of microRNA-194 on breast cancer cells via targeting F-box/WD repeat-containing protein 7

Promotional effect of microRNA-194 on breast cancer cells via targeting F-box/WD repeat-containing protein 7

microRNA-192, -194 and -215 are frequently downregulated in colorectal cancer

The nuclear receptor CAR modulates alcohol-induced liver injury

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