miR‑190b promotes colorectal cancer progression through targeting forkhead box protein P2

Zhao, Qiang; Liu, Chengkui; Cui, Qing; Luan, Xuerong; Wang, Qingfeng; Zhou, Chixing

doi:10.3892/etm.2019.8175

Cited by 9 publications

(7 citation statements)

References 15 publications

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“…In the present study, we identified 126 miRNAs downregulated by DDX17 suppression in SW620 cells, including miR-190b, miR-542-5p and miR-301a-3p. MiR-190b exhibited an oncogenic function in CRC and bladder cancer [ 32 , 33 ]. Increased expression of miR-542-5p was observed in the plasma of CRC patients compared with non-CRC subjects, and was associated with abnormal purine metabolism [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

DDX17 induces epithelial-mesenchymal transition and metastasis through the miR-149-3p/CYBRD1 pathway in colorectal cancer

et al. 2023

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DEAD box helicase 17 (DDX17) has been reported to be involved in the initiation and development of several cancers. However, the functional role and mechanisms of DDX17 in colorectal cancer (CRC) malignant progression and metastasis remain unclear. Here, we reported that DDX17 expression was increased in CRC tissues compared with noncancerous mucosa tissues and further upregulated in CRC liver metastasis compared with patient-paired primary tumors. High levels of DDX17 were significantly correlated with aggressive phenotypes and worse clinical outcomes in CRC patients. Ectopic expression of DDX17 promoted cell migration and invasion in vitro and in vivo, while the opposite results were obtained in DDX17-deficient CRC cells. We identified miR-149-3p as a potential downstream miRNA of DDX17 through RNA sequencing analysis, and miR-149-3p displayed a suppressive effect on the metastatic potential of CRC cells. We demonstrated that CYBRD1 (a ferric reductase that contributes to dietary iron absorption) was a direct target of miR-149-3p and that miR-149-3p was required for DDX17-mediated regulation of CYBRD1 expression. Moreover, DDX17 contributed to the metastasis and epithelial to mesenchymal transition (EMT) of CRC cells via downregulation of miR-149-3p, which resulted in increased CYBRD1 expression. In conclusion, our findings not only highlight the significance of DDX17 in the aggressive development and prognosis of CRC patients, but also reveal a novel mechanism underlying DDX17-mediated CRC cell metastasis and EMT progression through manipulation of the miR-149-3p/CYBRD1 pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

DDX17 induces epithelial-mesenchymal transition and metastasis through the miR-149-3p/CYBRD1 pathway in colorectal cancer

et al. 2023

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show abstract

“…In the present study, we identi ed 127 miRNAs downregulated by DDX17 suppression in SW620 cells, including miR-190b, miR-542-5p and miR-301a-3p. MiR-190b exhibited an oncogenic function in CRC and bladder cancer 32,33 . Increased expression of miR-542-5p was observed in the plasma of CRC patients compared with non-CRC subjects, and was associated with abnormal purine metabolism 34 .…”

Section: Discussionmentioning

confidence: 99%

DDX17 induces epithelial-mesenchymal transition and metastasis through the miR-149-3p/CYBRD1 pathway in colorectal cancer

Zhao

Zhang²,

et al. 2022

Preprint

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DEAD box helicase 17 (DDX17) has been reported to be involved in the initiation and development of several cancers. However, the functional role and mechanisms of DDX17 in colorectal cancer (CRC) malignant progression and metastasis remain unclear. Here, we reported that DDX17 expression was increased in CRC tissues compared with noncancerous mucosa tissues and further upregulated in CRC liver metastasis compared with patient-paired primary tumors. High levels of DDX17 were significantly correlated with aggressive phenotypes and worse clinical outcomes in CRC patients. Ectopic expression of DDX17 promoted cell migration and invasion in vitro and in vivo, while the opposite results were obtained in DDX17-deficient CRC cells. We identified miR-149-3p as a potential downstream miRNA of DDX17 through RNA sequencing analysis, and miR-149-3p displayed a suppressive effect on the metastatic potential of CRC cells. We demonstrated that CYBRD1 (a ferric reductase that contributes to dietary iron absorption) was a direct target of miR-149-3p and that miR-149-3p was required for DDX17-mediated regulation of CYBRD1 expression. Moreover, DDX17 contributed to the metastasis and epithelial to mesenchymal transition (EMT) of CRC cells via downregulation of miR-149-3p, which resulted in increased CYBRD1 expression. In conclusion, our ﬁndings not only highlight the significance of DDX17 in the aggressive development and prognosis of CRC patients, but also reveal a novel mechanism underlying DDX17-mediated CRC cell metastasis and EMT progression through manipulation of the miR-149-3p/CYBRD1 pathway.

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“…Generally, miR-375 acts as tumor suppressor, repressing many critical oncogenes such as PDK1, JAK2, IGF1R, and AEG-1 [ 34 ]. Less known, is miR-190b, although it is also engaged in promoting many cancers such as bladder carcinoma [ 35 ] or colorectal cancer [ 36 ] and found, as well, as a novel tumor suppressor in lung cancer during in vivo miRNA knockout screening [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Low Expression of miR-375 and miR-190b Differentiates Grade 3 Patients with Endometrial Cancer

et al. 2021

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Endometrial cancer (EC) is treated according to the stage and prognostic risk factors. Most EC patients are in the early stages and they are treated surgically. However, some of them, including those with high grade (grade 3) are in the intermediate and high intermediate prognostic risk groups and may require adjuvant therapy. The goal of the study was to find differences between grades based on an miRNA gene expression profile. Tumor samples from 24 patients with grade 1 (n = 10), 2 (n = 7), and 3 (n = 7) EC were subjected to miRNA profiling using next generation sequencing. The results obtained were validated using the miRNA profile of 407 EC tumors from the external Cancer Genome Atlas (TCGA) cohort. We obtained sets of differentially expressed (DE) miRNAs with the largest amount between G2 to G1 (50 transcripts) and G3 to G1 (40 transcripts) patients. Validation of our results with external data (TCGA) gave us a reasonable gene overlap of which we selected two miRNAs (miR-375 and miR190b) that distinguish the high grade best from the low grade EC. Unsupervised clustering showed a high degree of heterogeneity within grade 2 samples. MiR-375 as well as 190b might be useful to create grading verification test for high grade EC. One of the possible mechanisms that is responsible for the high grade is modulation by virus of host morphology or physiology.

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miR‑190b promotes colorectal cancer progression through targeting forkhead box protein P2

Cited by 9 publications

References 15 publications

DDX17 induces epithelial-mesenchymal transition and metastasis through the miR-149-3p/CYBRD1 pathway in colorectal cancer

DDX17 induces epithelial-mesenchymal transition and metastasis through the miR-149-3p/CYBRD1 pathway in colorectal cancer

DDX17 induces epithelial-mesenchymal transition and metastasis through the miR-149-3p/CYBRD1 pathway in colorectal cancer

Low Expression of miR-375 and miR-190b Differentiates Grade 3 Patients with Endometrial Cancer

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